TY - JOUR
T1 - Arabidopsis PLDs with C2‐domain function distinctively in hypoxia
AU - Premkumar, Albert
AU - Lindberg, Sylvia
AU - Lager, Ida
AU - Rasmussen, Ulla
AU - Schulz, Alexander
PY - 2019/9
Y1 - 2019/9
N2 - Hypoxia (oxygen deprivation) causes metabolic disturbances at physiological, biochemical and genetic levels and results in decreased plant growth and development. Phospholipase D (PLD)-mediated signaling was reported for abiotic and biotic stress signaling events in plants. To investigate the participatory role of PLDs also in hypoxia signaling, we used wild type of Arabidopsis thaliana and 10 pld isoform mutants containing C2-domain. Hypoxia-induced changes in three major signaling players, namely, cytosolic free calcium (Ca2+
cyt), reactive oxygen species (ROS) and phosphatidic acid (PA), were determined in mesophyll protoplasts. The Ca2+
cyt and ROS levels were monitored by fluorescence microscopy and confocal imaging, while PA levels were quantified by an enzymatic method. Our findings reveal that the elevations of cytosolic calcium and PA are reduced in all the 10 mutants dysfunctional in PLD isoforms. The hypoxia-related changes in both calcium and ROS show different kinetic patterns depending on the type of PLD studied. Pharmacological experiments confirm that both external and internal sources contribute to calcium and ROS accumulation under hypoxia. PLDα1-3, PLDβ1 and PLDγ1-3 are likely involved in calcium signaling under hypoxia as well as in PA production, while all investigated PLDs, except for PLDγ3, take part in ROS elevation.
AB - Hypoxia (oxygen deprivation) causes metabolic disturbances at physiological, biochemical and genetic levels and results in decreased plant growth and development. Phospholipase D (PLD)-mediated signaling was reported for abiotic and biotic stress signaling events in plants. To investigate the participatory role of PLDs also in hypoxia signaling, we used wild type of Arabidopsis thaliana and 10 pld isoform mutants containing C2-domain. Hypoxia-induced changes in three major signaling players, namely, cytosolic free calcium (Ca2+
cyt), reactive oxygen species (ROS) and phosphatidic acid (PA), were determined in mesophyll protoplasts. The Ca2+
cyt and ROS levels were monitored by fluorescence microscopy and confocal imaging, while PA levels were quantified by an enzymatic method. Our findings reveal that the elevations of cytosolic calcium and PA are reduced in all the 10 mutants dysfunctional in PLD isoforms. The hypoxia-related changes in both calcium and ROS show different kinetic patterns depending on the type of PLD studied. Pharmacological experiments confirm that both external and internal sources contribute to calcium and ROS accumulation under hypoxia. PLDα1-3, PLDβ1 and PLDγ1-3 are likely involved in calcium signaling under hypoxia as well as in PA production, while all investigated PLDs, except for PLDγ3, take part in ROS elevation.
UR - http://www.scopus.com/inward/record.url?scp=85058012023&partnerID=8YFLogxK
U2 - 10.1111/ppl.12874
DO - 10.1111/ppl.12874
M3 - Journal article
C2 - 30417386
AN - SCOPUS:85058012023
SN - 0031-9317
VL - 167
SP - 90
EP - 110
JO - Physiologia Plantarum
JF - Physiologia Plantarum
IS - 1
ER -