TY - JOUR
T1 - Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth
AU - Pratt-Riccio, Lilian Rose
AU - Bianco, Cesare
AU - Totino, Paulo Renato Rivas
AU - Perce-Da-Silva, Daiana De Souza
AU - Silva, Luciene Aquino
AU - Riccio, Evelyn Kety Pratt
AU - Ennes-Vidal, Vítor
AU - Neves-Ferreira, Ana Gisele Costa
AU - Perales, Jonas
AU - Da Rocha, Surza Lucia Gonçalves
AU - Dias-Da-Silva, Fabrício
AU - Ferreira-da-Cruz, Maria de Fátima
AU - Daniel-Ribeiro, Cláudio Tadeu
AU - De Oliveira-Ferreira, Joseli
AU - Theisen, Michael
AU - Carvalho, Leonardo José De Moura
AU - Banic, Dalma Maria
PY - 2011/8
Y1 - 2011/8
N2 - The glutamate-rich protein (GLURP) is an exoantigen expressed in all stages of the Plasmodium falciparum life cycle in humans. Anti-GLURP antibodies can inhibit parasite growth in the presence of monocytes via antibody-dependent cellular inhibition (ADCI), and a major parasite-inhibitory region has been found in the N-terminal R0 region of the protein. Herein, we describe the antiplasmodial activity of anti-GLURP antibodies present in the sera from individuals naturally exposed to malaria in a Brazilian malaria-endemic area. The anti-R0 antibodies showed a potent inhibitory effect on the growth of P. falciparum in vitro, both in the presence (ADCI) and absence (GI) of monocytes. The inhibitory effect on parasite growth was comparable to the effect of IgGs purified from pooled sera from hyperimmune African individuals. Interestingly, in the ADCI test, higher levels of tumour necrosis factor alpha (TNF-α) were observed in the supernatant from cultures with higher parasitemias. Our data suggest that the antibody response induced by GLURP-R0 in naturally exposed individuals may have an important role in controlling parasitemia because these antibodies are able to inhibit the in vitro growth of P. falciparum with or without the cooperation from monocytes. Our results also indicate that TNF-α may not be relevant for the inhibitory effect on P. falciparum in vitro growth.
AB - The glutamate-rich protein (GLURP) is an exoantigen expressed in all stages of the Plasmodium falciparum life cycle in humans. Anti-GLURP antibodies can inhibit parasite growth in the presence of monocytes via antibody-dependent cellular inhibition (ADCI), and a major parasite-inhibitory region has been found in the N-terminal R0 region of the protein. Herein, we describe the antiplasmodial activity of anti-GLURP antibodies present in the sera from individuals naturally exposed to malaria in a Brazilian malaria-endemic area. The anti-R0 antibodies showed a potent inhibitory effect on the growth of P. falciparum in vitro, both in the presence (ADCI) and absence (GI) of monocytes. The inhibitory effect on parasite growth was comparable to the effect of IgGs purified from pooled sera from hyperimmune African individuals. Interestingly, in the ADCI test, higher levels of tumour necrosis factor alpha (TNF-α) were observed in the supernatant from cultures with higher parasitemias. Our data suggest that the antibody response induced by GLURP-R0 in naturally exposed individuals may have an important role in controlling parasitemia because these antibodies are able to inhibit the in vitro growth of P. falciparum with or without the cooperation from monocytes. Our results also indicate that TNF-α may not be relevant for the inhibitory effect on P. falciparum in vitro growth.
KW - Adolescent
KW - Adult
KW - Aged
KW - Antibodies, Protozoan
KW - Endemic Diseases
KW - Enzyme-Linked Immunosorbent Assay
KW - Fluorescent Antibody Technique, Indirect
KW - Humans
KW - Immunoglobulin G
KW - Malaria, Falciparum
KW - Middle Aged
KW - Parasitemia
KW - Plasmodium falciparum
KW - Protozoan Proteins
KW - Tumor Necrosis Factor-alpha
KW - Young Adult
M3 - Journal article
C2 - 21881755
SN - 0074-0276
VL - 106 Suppl 1
SP - 34
EP - 43
JO - Memórias do Instituto Oswaldo Cruz
JF - Memórias do Instituto Oswaldo Cruz
ER -
