Antibodies against PfEMP1, RIFIN, MSP3 and GLURP are acquired during controlled Plasmodium falciparum malaria infections in naïve volunteers

TY - JOUR

T1 - Antibodies against PfEMP1, RIFIN, MSP3 and GLURP are acquired during controlled Plasmodium falciparum malaria infections in naïve volunteers

AU - Turner, Louise

AU - Wang, Christian W

AU - Lavstsen, Thomas

AU - Mwakalinga, Steven B

AU - Sauerwein, Robert W

AU - Hermsen, Cornelus C

AU - Theander, Thor G

PY - 2011

Y1 - 2011

N2 - Antibodies to polymorphic antigens expressed during the parasites erythrocytic stages are important mediators of protective immunity against P. falciparum malaria. Therefore, polymorphic blood stage antigens like MSP3, EBA-175 and GLURP and variant surface antigens PfEMP1 and RIFIN are considered vaccine candidates. However, to what extent these antibodies to blood stage antigens are acquired during naive individuals' first infections has not been studied in depth. Using plasma samples collected from controlled experimental P. falciparum infections we show that antibodies against variant surface antigens, PfEMP1 and RIFIN as well as MSP3 and GLURP, are acquired during a single short low density P. falciparum infection in non-immune individuals including strain transcendent PfEMP1 immune responses. These data indicate that the immunogenicity of the variant surface antigens is similar to the less diverse merozoite antigens. The acquisition of a broad and strain transcendent repertoire of PfEMP1 antibodies may reflect a parasite strategy of expressing most or all PfEMP1 variants at liver release optimizing the likelihood of survival and establishment of chronic infections in the new host.

AB - Antibodies to polymorphic antigens expressed during the parasites erythrocytic stages are important mediators of protective immunity against P. falciparum malaria. Therefore, polymorphic blood stage antigens like MSP3, EBA-175 and GLURP and variant surface antigens PfEMP1 and RIFIN are considered vaccine candidates. However, to what extent these antibodies to blood stage antigens are acquired during naive individuals' first infections has not been studied in depth. Using plasma samples collected from controlled experimental P. falciparum infections we show that antibodies against variant surface antigens, PfEMP1 and RIFIN as well as MSP3 and GLURP, are acquired during a single short low density P. falciparum infection in non-immune individuals including strain transcendent PfEMP1 immune responses. These data indicate that the immunogenicity of the variant surface antigens is similar to the less diverse merozoite antigens. The acquisition of a broad and strain transcendent repertoire of PfEMP1 antibodies may reflect a parasite strategy of expressing most or all PfEMP1 variants at liver release optimizing the likelihood of survival and establishment of chronic infections in the new host.

KW - Animals

KW - Antibodies, Protozoan

KW - Antigens, Protozoan

KW - Human Experimentation

KW - Humans

KW - Immunization

KW - Immunoglobulin G

KW - Life Cycle Stages

KW - Liver

KW - Malaria, Falciparum

KW - Membrane Proteins

KW - Plasmodium falciparum

KW - Protein Structure, Tertiary

KW - Protozoan Proteins

U2 - 10.1371/journal.pone.0029025

DO - 10.1371/journal.pone.0029025

M3 - Journal article

C2 - 22174947

SN - 1932-6203

VL - 6

SP - e29025

JO - PLoS Computational Biology

JF - PLoS Computational Biology

IS - 12

ER -