TY - JOUR
T1 - Antibacterial peptides generated by Alcalase hydrolysis of goat whey
AU - Osman, Ali
AU - Goda, Hanan A
AU - Abdel-Hamid, Mahmoud
AU - Badran, Sanaa M.
AU - Otte, Jeanette
PY - 2016
Y1 - 2016
N2 - Goat whey hydrolysates were produced from goat whey using Alcalase® from Bacillus licheniformis. The goat whey hydrolysate (GWH) with the highest degree of hydrolysis was fractionated by size exclusion chromatography (SEC), and the antibacterial activity of the GWH and the obtained SEC-fractions were evaluated using the disc diffusion method. The results showed that the GWH exhibited significantly higher antibacterial activity than the unhydrolysed goat whey. Among all SEC fractions obtained, only SEC-F2 and SEC-F3 showed antibacterial activity against the tested bacteria. SEC-F3 exhibited the highest antibacterial effectiveness against Escherichia coli and Bacillus cereus with MIC values of 0.09 and 0.03 mg/mL respectively, whereas, SEC-F3 and SEC-F2 had comparable MIC values against Staphylococcus aureus (~0.02 mg/mL). Transmission electron microscopy showed that the GWH caused changes in the bacterial cells affected. Additionally, LC-MS analysis was used to characterize the peptides of GWH and SEC-F3. Two major peptides were detected in GWH and SEC-F3, with higher concentration in the latter, having masses of 730 and 1183 Da, respectively. These results suggest that hydrolysis of goat whey by Alcalase is an easy tool to enhance its antibacterial activities. GWH and its effective antibacterial fractions may be used to control the undesirable bacteria in food and improve its safety.
AB - Goat whey hydrolysates were produced from goat whey using Alcalase® from Bacillus licheniformis. The goat whey hydrolysate (GWH) with the highest degree of hydrolysis was fractionated by size exclusion chromatography (SEC), and the antibacterial activity of the GWH and the obtained SEC-fractions were evaluated using the disc diffusion method. The results showed that the GWH exhibited significantly higher antibacterial activity than the unhydrolysed goat whey. Among all SEC fractions obtained, only SEC-F2 and SEC-F3 showed antibacterial activity against the tested bacteria. SEC-F3 exhibited the highest antibacterial effectiveness against Escherichia coli and Bacillus cereus with MIC values of 0.09 and 0.03 mg/mL respectively, whereas, SEC-F3 and SEC-F2 had comparable MIC values against Staphylococcus aureus (~0.02 mg/mL). Transmission electron microscopy showed that the GWH caused changes in the bacterial cells affected. Additionally, LC-MS analysis was used to characterize the peptides of GWH and SEC-F3. Two major peptides were detected in GWH and SEC-F3, with higher concentration in the latter, having masses of 730 and 1183 Da, respectively. These results suggest that hydrolysis of goat whey by Alcalase is an easy tool to enhance its antibacterial activities. GWH and its effective antibacterial fractions may be used to control the undesirable bacteria in food and improve its safety.
U2 - 10.1016/j.lwt.2015.08.043
DO - 10.1016/j.lwt.2015.08.043
M3 - Journal article
SN - 0023-6438
VL - 65
SP - 480
EP - 486
JO - L W T- Food Science and Technology
JF - L W T- Food Science and Technology
ER -