Antenatal bestemmelse af føtal RhD-blodtype baseret på føtalt DNA i plasma fra den RhD-negative mor - Sekundærpublikation

Frederik Banch Clausen; Grethe Risum Krog; Klaus Rieneck; Leif Kofoed Nielsen; Rasmus Lundquist; Kirstin Finning; Ebbe Dickmeiss; Morten Hedegaard; Morten Hanefeld Dziegiel

Antenatal bestemmelse af føtal RhD-blodtype baseret på føtalt DNA i plasma fra den RhD-negative mor - Sekundærpublikation

Translated title of the contribution: Antenatal determination of fetal RhD-blood type based on fetal DNA in plasma from the RhD-negative mother

Frederik Banch Clausen^*, Grethe Risum Krog, Klaus Rieneck, Leif Kofoed Nielsen, Rasmus Lundquist, Kirstin Finning, Ebbe Dickmeiss, Morten Hedegaard, Morten Hanefeld Dziegiel

^*Corresponding author for this work

2 Citations (Scopus)

Abstract

We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD- women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15^th -36 ^th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.

Translated title of the contribution	Antenatal determination of fetal RhD-blood type based on fetal DNA in plasma from the RhD-negative mother
Original language	Danish
Journal	Ugeskrift for Laeger
Volume	168
Issue number	26-32
Pages (from-to)	2568-2570
Number of pages	3
ISSN	0041-5782
Publication status	Published - 26 Jun 2006

Cite this

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title = "Antenatal bestemmelse af f{\o}tal RhD-blodtype baseret p{\aa} f{\o}talt DNA i plasma fra den RhD-negative mor - Sekund{\ae}rpublikation",

abstract = "We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD- women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th -36 th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.",

author = "Clausen, {Frederik Banch} and Krog, {Grethe Risum} and Klaus Rieneck and Nielsen, {Leif Kofoed} and Rasmus Lundquist and Kirstin Finning and Ebbe Dickmeiss and Morten Hedegaard and Dziegiel, {Morten Hanefeld}",

year = "2006",

month = jun,

day = "26",

language = "Dansk",

volume = "168",

pages = "2568--2570",

journal = "Ugeskrift for Laeger",

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publisher = "Almindelige Danske Laegeforening",

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TY - JOUR

T1 - Antenatal bestemmelse af føtal RhD-blodtype baseret på føtalt DNA i plasma fra den RhD-negative mor - Sekundærpublikation

AU - Clausen, Frederik Banch

AU - Krog, Grethe Risum

AU - Rieneck, Klaus

AU - Nielsen, Leif Kofoed

AU - Lundquist, Rasmus

AU - Finning, Kirstin

AU - Dickmeiss, Ebbe

AU - Hedegaard, Morten

AU - Dziegiel, Morten Hanefeld

PY - 2006/6/26

Y1 - 2006/6/26

N2 - We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD- women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th -36 th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.

AB - We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD- women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th -36 th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.

UR - http://www.scopus.com/inward/record.url?scp=33746581408&partnerID=8YFLogxK

M3 - Tidsskriftartikel

C2 - 16824414

AN - SCOPUS:33746581408

SN - 0041-5782

VL - 168

SP - 2568

EP - 2570

JO - Ugeskrift for Laeger

JF - Ugeskrift for Laeger

IS - 26-32

ER -

Antenatal bestemmelse af føtal RhD-blodtype baseret på føtalt DNA i plasma fra den RhD-negative mor - Sekundærpublikation

Abstract

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