Antagonistic actions of ecdysone and insulins determine final size in Drosophila

Julien Colombani; Laurence Bianchini; Sophie Layalle; Emilie Pondeville; Chantal Dauphin-Villemant; Christophe Antoniewski; Clément Carré; Stéphane Noselli; Pierre Léopold

doi:10.1126/science.1119432

Antagonistic actions of ecdysone and insulins determine final size in Drosophila

Julien Colombani, Laurence Bianchini, Sophie Layalle, Emilie Pondeville, Chantal Dauphin-Villemant, Christophe Antoniewski, Clément Carré, Stéphane Noselli, Pierre Léopold

426 Citations (Scopus)

Abstract

All animals coordinate growth and maturation to reach their final size and shape. In insects, insulin family molecules control growth and metabolism, whereas pulses of the steroid 20-hydroxyecdysone (20E) initiate major developmental transitions. We show that 20E signaling also negatively controls animal growth rates by impeding general insulin signaling involving localization of the transcription factor dFOXO and transcription of the translation inhibitor 4E-BP. We also demonstrate that the larval fat body, equivalent to the vertebrate liver, is a key relay element for ecdysone-dependent growth inhibition. Hence, ecdysone counteracts the growth-promoting action of insulins, thus forming a humoral regulatory loop that determines organismal size.

Original language	English
Journal	Science (New York, N.Y.)
Volume	310
Issue number	5748
Pages (from-to)	667-70
Number of pages	4
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.1119432
Publication status	Published - 28 Oct 2005
Externally published	Yes

Keywords

Animals
Body Size
Crosses, Genetic
DNA-Binding Proteins/genetics
Drosophila melanogaster/growth & development
Ecdysterone/physiology
Fat Body/physiology
Insect Proteins/physiology
Insulin/physiology
Insulin Antagonists
Larva/growth & development
Phosphatidylinositol 3-Kinases/genetics
Signal Transduction
Transcription Factors/genetics

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10.1126/science.1119432

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title = "Antagonistic actions of ecdysone and insulins determine final size in Drosophila",

abstract = "All animals coordinate growth and maturation to reach their final size and shape. In insects, insulin family molecules control growth and metabolism, whereas pulses of the steroid 20-hydroxyecdysone (20E) initiate major developmental transitions. We show that 20E signaling also negatively controls animal growth rates by impeding general insulin signaling involving localization of the transcription factor dFOXO and transcription of the translation inhibitor 4E-BP. We also demonstrate that the larval fat body, equivalent to the vertebrate liver, is a key relay element for ecdysone-dependent growth inhibition. Hence, ecdysone counteracts the growth-promoting action of insulins, thus forming a humoral regulatory loop that determines organismal size.",

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author = "Julien Colombani and Laurence Bianchini and Sophie Layalle and Emilie Pondeville and Chantal Dauphin-Villemant and Christophe Antoniewski and Cl{\'e}ment Carr{\'e} and St{\'e}phane Noselli and Pierre L{\'e}opold",

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doi = "10.1126/science.1119432",

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T1 - Antagonistic actions of ecdysone and insulins determine final size in Drosophila

AU - Colombani, Julien

AU - Bianchini, Laurence

AU - Layalle, Sophie

AU - Pondeville, Emilie

AU - Dauphin-Villemant, Chantal

AU - Antoniewski, Christophe

AU - Carré, Clément

AU - Noselli, Stéphane

AU - Léopold, Pierre

PY - 2005/10/28

Y1 - 2005/10/28

N2 - All animals coordinate growth and maturation to reach their final size and shape. In insects, insulin family molecules control growth and metabolism, whereas pulses of the steroid 20-hydroxyecdysone (20E) initiate major developmental transitions. We show that 20E signaling also negatively controls animal growth rates by impeding general insulin signaling involving localization of the transcription factor dFOXO and transcription of the translation inhibitor 4E-BP. We also demonstrate that the larval fat body, equivalent to the vertebrate liver, is a key relay element for ecdysone-dependent growth inhibition. Hence, ecdysone counteracts the growth-promoting action of insulins, thus forming a humoral regulatory loop that determines organismal size.

AB - All animals coordinate growth and maturation to reach their final size and shape. In insects, insulin family molecules control growth and metabolism, whereas pulses of the steroid 20-hydroxyecdysone (20E) initiate major developmental transitions. We show that 20E signaling also negatively controls animal growth rates by impeding general insulin signaling involving localization of the transcription factor dFOXO and transcription of the translation inhibitor 4E-BP. We also demonstrate that the larval fat body, equivalent to the vertebrate liver, is a key relay element for ecdysone-dependent growth inhibition. Hence, ecdysone counteracts the growth-promoting action of insulins, thus forming a humoral regulatory loop that determines organismal size.

KW - Animals

KW - Body Size

KW - Crosses, Genetic

KW - DNA-Binding Proteins/genetics

KW - Drosophila melanogaster/growth & development

KW - Ecdysterone/physiology

KW - Fat Body/physiology

KW - Insect Proteins/physiology

KW - Insulin/physiology

KW - Insulin Antagonists

KW - Larva/growth & development

KW - Phosphatidylinositol 3-Kinases/genetics

KW - Signal Transduction

KW - Transcription Factors/genetics

U2 - 10.1126/science.1119432

DO - 10.1126/science.1119432

M3 - Journal article

C2 - 16179433

SN - 0036-8075

VL - 310

SP - 667

EP - 670

JO - Science

JF - Science

IS - 5748

ER -

Antagonistic actions of ecdysone and insulins determine final size in Drosophila

Abstract

Keywords

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