Anion conductance of the human red cell is carried by a maxi-anion channel

Edyta Glogowska; Agnieszka Dyrda; Anne Cueff; Guillaume Bouyer; Stéphane Egée; Poul Bennekou; Serge L. Y. Thomas

doi:10.1016/j.bcmd.2010.02.014

Anion conductance of the human red cell is carried by a maxi-anion channel

Edyta Glogowska, Agnieszka Dyrda, Anne Cueff, Guillaume Bouyer, Stéphane Egée, Poul Bennekou, Serge L. Y. Thomas

Molecular Integrative Physiology

12 Citations (Scopus)

Abstract

Historically, the anion transport through the human red cell membrane has been perceived to be mediated by Band 3, in the two-component concept with the large electroneutral anion exchange accompanied by the conductance proper, which dominated the total membrane conductance. The status of anion channels proper has never been clarified, and the informations obtained by different groups of electrophysiologists are rather badly matched. This study, using the cell-attached configuration of the patch-clamp technique, rationalizes and explains earlier confusing results by demonstrating that the diversity of anionic channel activities recorded in human erythrocytes corresponds to different kinetic modalities of a unique type of maxi-anion channel with multiple conductance levels and probably multiple gating properties and pharmacology, depending on conditions. It demonstrates the role of activator played by serum in the recruitment of multiple new conductance levels showing very complex kinetics and gating properties upon serum addition. These channels, which seem to be dormant under normal physiological conditions, are potentially activable and could confer a far higher anion conductance to the red cell than the ground leak mediated by Band 3.

Original language	English
Journal	Blood Cells, Molecules and Diseases
Volume	44
Issue number	4
Pages (from-to)	243-251
Number of pages	8
ISSN	1079-9796
DOIs	https://doi.org/10.1016/j.bcmd.2010.02.014
Publication status	Published - Apr 2010

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title = "Anion conductance of the human red cell is carried by a maxi-anion channel",

abstract = "Historically, the anion transport through the human red cell membrane has been perceived to be mediated by Band 3, in the two-component concept with the large electroneutral anion exchange accompanied by the conductance proper, which dominated the total membrane conductance. The status of anion channels proper has never been clarified, and the informations obtained by different groups of electrophysiologists are rather badly matched. This study, using the cell-attached configuration of the patch-clamp technique, rationalizes and explains earlier confusing results by demonstrating that the diversity of anionic channel activities recorded in human erythrocytes corresponds to different kinetic modalities of a unique type of maxi-anion channel with multiple conductance levels and probably multiple gating properties and pharmacology, depending on conditions. It demonstrates the role of activator played by serum in the recruitment of multiple new conductance levels showing very complex kinetics and gating properties upon serum addition. These channels, which seem to be dormant under normal physiological conditions, are potentially activable and could confer a far higher anion conductance to the red cell than the ground leak mediated by Band 3.",

author = "Edyta Glogowska and Agnieszka Dyrda and Anne Cueff and Guillaume Bouyer and St{\'e}phane Eg{\'e}e and Poul Bennekou and Thomas, {Serge L. Y.}",

note = "Keywords: Human red blood cell; Membrane; Maxi-anion channels; Serum",

year = "2010",

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doi = "10.1016/j.bcmd.2010.02.014",

language = "English",

volume = "44",

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journal = "Blood Cells, Molecules and Diseases",

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T1 - Anion conductance of the human red cell is carried by a maxi-anion channel

AU - Glogowska, Edyta

AU - Dyrda, Agnieszka

AU - Cueff, Anne

AU - Bouyer, Guillaume

AU - Egée, Stéphane

AU - Bennekou, Poul

AU - Thomas, Serge L. Y.

N1 - Keywords: Human red blood cell; Membrane; Maxi-anion channels; Serum

PY - 2010/4

Y1 - 2010/4

N2 - Historically, the anion transport through the human red cell membrane has been perceived to be mediated by Band 3, in the two-component concept with the large electroneutral anion exchange accompanied by the conductance proper, which dominated the total membrane conductance. The status of anion channels proper has never been clarified, and the informations obtained by different groups of electrophysiologists are rather badly matched. This study, using the cell-attached configuration of the patch-clamp technique, rationalizes and explains earlier confusing results by demonstrating that the diversity of anionic channel activities recorded in human erythrocytes corresponds to different kinetic modalities of a unique type of maxi-anion channel with multiple conductance levels and probably multiple gating properties and pharmacology, depending on conditions. It demonstrates the role of activator played by serum in the recruitment of multiple new conductance levels showing very complex kinetics and gating properties upon serum addition. These channels, which seem to be dormant under normal physiological conditions, are potentially activable and could confer a far higher anion conductance to the red cell than the ground leak mediated by Band 3.

AB - Historically, the anion transport through the human red cell membrane has been perceived to be mediated by Band 3, in the two-component concept with the large electroneutral anion exchange accompanied by the conductance proper, which dominated the total membrane conductance. The status of anion channels proper has never been clarified, and the informations obtained by different groups of electrophysiologists are rather badly matched. This study, using the cell-attached configuration of the patch-clamp technique, rationalizes and explains earlier confusing results by demonstrating that the diversity of anionic channel activities recorded in human erythrocytes corresponds to different kinetic modalities of a unique type of maxi-anion channel with multiple conductance levels and probably multiple gating properties and pharmacology, depending on conditions. It demonstrates the role of activator played by serum in the recruitment of multiple new conductance levels showing very complex kinetics and gating properties upon serum addition. These channels, which seem to be dormant under normal physiological conditions, are potentially activable and could confer a far higher anion conductance to the red cell than the ground leak mediated by Band 3.

U2 - 10.1016/j.bcmd.2010.02.014

DO - 10.1016/j.bcmd.2010.02.014

M3 - Journal article

C2 - 20226698

SN - 1079-9796

VL - 44

SP - 243

EP - 251

JO - Blood Cells, Molecules and Diseases

JF - Blood Cells, Molecules and Diseases

IS - 4

ER -

Anion conductance of the human red cell is carried by a maxi-anion channel

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