Ancient conservation of trinucleotide microsatellite loci in polistine wasps

TY - JOUR

T1 - Ancient conservation of trinucleotide microsatellite loci in polistine wasps

AU - Ezenwa, V O

AU - Peters, J M

AU - Zhu, Y

AU - Arévalo, E

AU - Hastings, M D

AU - Seppä, P

AU - Pedersen, Jes Søe

AU - Zacchi, F

AU - Queller, D C

AU - Strassmann, J E

N1 - Copyright 1998 Academic Press.

PY - 1998/10

Y1 - 1998/10

N2 - Microsatellites have proven to be very useful genetic markers for studies of kinship, parentage, and gene mapping. If microsatellites are conserved among species, then those developed for one species can be used on related species, which would save the time and effort of developing new loci. We evaluated conservation of 27 trinucleotide loci that were derived from 2 species of Polistes wasps in cross-species applications on 27 species chosen from the major lineages of the Vespidae, which diverged as much as 144 million years ago. We further investigated cross-species polymorphism levels for 18 of the loci. There was a clear relationship between cladistic distance and both conservation of the priming sites and heterozygosity. However the loci derived from P. bellicosus were much more widely conserved and polymorphic than were those derived from P. annularis. The disparity in cross-species utility between these sets of loci means that caution should be used in generalizing from conservation rates derived from single species. We found no relationship between locus conservation or heterozygosity and GC content of flanks, repeat motif, repeat length, or heterozygosity in the original species, which suggests that generalizations from other studies reporting such patterns are premature.

AB - Microsatellites have proven to be very useful genetic markers for studies of kinship, parentage, and gene mapping. If microsatellites are conserved among species, then those developed for one species can be used on related species, which would save the time and effort of developing new loci. We evaluated conservation of 27 trinucleotide loci that were derived from 2 species of Polistes wasps in cross-species applications on 27 species chosen from the major lineages of the Vespidae, which diverged as much as 144 million years ago. We further investigated cross-species polymorphism levels for 18 of the loci. There was a clear relationship between cladistic distance and both conservation of the priming sites and heterozygosity. However the loci derived from P. bellicosus were much more widely conserved and polymorphic than were those derived from P. annularis. The disparity in cross-species utility between these sets of loci means that caution should be used in generalizing from conservation rates derived from single species. We found no relationship between locus conservation or heterozygosity and GC content of flanks, repeat motif, repeat length, or heterozygosity in the original species, which suggests that generalizations from other studies reporting such patterns are premature.

KW - Animals

KW - Cold Temperature

KW - DNA

KW - Polymerase Chain Reaction

KW - Polymorphism, Genetic

KW - Species Specificity

KW - Trinucleotide Repeats

KW - Wasps

U2 - 10.1006/mpev.1998.0528

DO - 10.1006/mpev.1998.0528

M3 - Journal article

C2 - 9878228

SN - 1055-7903

VL - 10

SP - 168

EP - 177

JO - Molecular Phylogenetics and Evolution

JF - Molecular Phylogenetics and Evolution

IS - 2

ER -