Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase from Bacillus subtilis

C L Pearson; C A Loshon; P Setlow; Lotte Bang Pedersen; Barbara Setlow

Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase from Bacillus subtilis

C L Pearson, C A Loshon, P Setlow, Lotte Bang Pedersen, Barbara Setlow

9 Citations (Scopus)

Abstract

A Bacillus subtilis gene termed yhfR encodes the only B. subtilis protein with significant sequence similarity to 2, 3-diphosphoglycerate-dependent phosphoglycerate mutases (dPGM). This gene is expressed at a low level during growth and sporulation, but deletion of yhfR had no effect on growth, sporulation, or spore germination and outgrowth. YhfR was expressed in and partially purified from Escherichia coli but had little if any PGM activity and gave no detectable PGM activity in B. subtilis. These data indicate that B. subtilis does not require YhfR and most likely does not require a dPGM.

Original language	English
Journal	Journal of Bacteriology
Volume	182
Issue number	14
Pages (from-to)	4121-3
Number of pages	2
ISSN	0021-9193
Publication status	Published - 2000
Externally published	Yes

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Pearson, C. L., Loshon, C. A., Setlow, P., Pedersen, L. B., & Setlow, B. (2000). Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase from Bacillus subtilis. Journal of Bacteriology, 182(14), 4121-3. http://jb.asm.org/cgi/content/full/182/14/4121?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=lacz&searchid=1&FIRSTINDEX=940&resourcetype=HWFIG#FN150

Pearson, CL, Loshon, CA, Setlow, P, Pedersen, LB & Setlow, B 2000, 'Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase from Bacillus subtilis', Journal of Bacteriology, vol. 182, no. 14, pp. 4121-3. <http://jb.asm.org/cgi/content/full/182/14/4121?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=lacz&searchid=1&FIRSTINDEX=940&resourcetype=HWFIG#FN150>

@article{8de4c630e07811dcbee902004c4f4f50,

title = "Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase from Bacillus subtilis",

abstract = "A Bacillus subtilis gene termed yhfR encodes the only B. subtilis protein with significant sequence similarity to 2, 3-diphosphoglycerate-dependent phosphoglycerate mutases (dPGM). This gene is expressed at a low level during growth and sporulation, but deletion of yhfR had no effect on growth, sporulation, or spore germination and outgrowth. YhfR was expressed in and partially purified from Escherichia coli but had little if any PGM activity and gave no detectable PGM activity in B. subtilis. These data indicate that B. subtilis does not require YhfR and most likely does not require a dPGM.",

author = "Pearson, {C L} and Loshon, {C A} and P Setlow and Pedersen, {Lotte Bang} and Barbara Setlow",

note = "Keywords: 2,3-Diphosphoglycerate; Bacillus subtilis; Phosphoglycerate Mutase; Recombinant Fusion Proteins; Spores, Bacterial",

year = "2000",

language = "English",

volume = "182",

pages = "4121--3",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "14",

}

TY - JOUR

T1 - Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase from Bacillus subtilis

AU - Pearson, C L

AU - Loshon, C A

AU - Setlow, P

AU - Pedersen, Lotte Bang

AU - Setlow, Barbara

N1 - Keywords: 2,3-Diphosphoglycerate; Bacillus subtilis; Phosphoglycerate Mutase; Recombinant Fusion Proteins; Spores, Bacterial

PY - 2000

Y1 - 2000

N2 - A Bacillus subtilis gene termed yhfR encodes the only B. subtilis protein with significant sequence similarity to 2, 3-diphosphoglycerate-dependent phosphoglycerate mutases (dPGM). This gene is expressed at a low level during growth and sporulation, but deletion of yhfR had no effect on growth, sporulation, or spore germination and outgrowth. YhfR was expressed in and partially purified from Escherichia coli but had little if any PGM activity and gave no detectable PGM activity in B. subtilis. These data indicate that B. subtilis does not require YhfR and most likely does not require a dPGM.

AB - A Bacillus subtilis gene termed yhfR encodes the only B. subtilis protein with significant sequence similarity to 2, 3-diphosphoglycerate-dependent phosphoglycerate mutases (dPGM). This gene is expressed at a low level during growth and sporulation, but deletion of yhfR had no effect on growth, sporulation, or spore germination and outgrowth. YhfR was expressed in and partially purified from Escherichia coli but had little if any PGM activity and gave no detectable PGM activity in B. subtilis. These data indicate that B. subtilis does not require YhfR and most likely does not require a dPGM.

M3 - Journal article

C2 - 10869096

SN - 0021-9193

VL - 182

SP - 4121

EP - 4123

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 14

ER -

Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase from Bacillus subtilis

Abstract

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