Analysis of gene expression in normal and neoplastic human testis: new roles of RNA

TY - JOUR

T1 - Analysis of gene expression in normal and neoplastic human testis: new roles of RNA

AU - Novotny, G W

AU - Nielsen, J E

AU - Sonne, Si Brask

AU - Skakkebaek, N E

AU - Rajpert-De Meyts, E

AU - Leffers, H

AU - Sonne, Si Brask

N1 - Keywords: Carcinoma in Situ; Cloning, Molecular; DNA, Neoplasm; Gene Expression Profiling; Gene Expression Regulation; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; In Situ Hybridization; Male; MicroRNAs; Oligonucleotide Array Sequence Analysis; RNA; RNA, Neoplasm; Testicular Neoplasms; Testis

PY - 2007

Y1 - 2007

N2 - Large-scale methods for analysing gene expression, such as microarrays, have yielded a wealth of information about gene expression at the mRNA level. However, expression of alternative transcripts, together with the presence of a wide range of largely undescribed RNA transcripts combined with regulation from the RNA interference pathway, may cause misinterpretations when trying to base conclusions from expression data derived from studies at the mRNA level. With HLXB9, PRM1, DICER and E2F1 as examples, we here show a range of situations that can occur when investigating gene expression, and give recommendations for the complementary methods that can verify gene expression data from large-scale studies, as well as give new information regarding the regulation of specific genes. Especially, we show that the absence of a protein despite high expression of the corresponding mRNA can be caused by expression of miRNAs targeting the mRNA. Additionally, we show through cloning the presence of both known and new miRNAs in the testis emphasizing the necessity for following up mRNA expression data by investigating expression at the protein level.

AB - Large-scale methods for analysing gene expression, such as microarrays, have yielded a wealth of information about gene expression at the mRNA level. However, expression of alternative transcripts, together with the presence of a wide range of largely undescribed RNA transcripts combined with regulation from the RNA interference pathway, may cause misinterpretations when trying to base conclusions from expression data derived from studies at the mRNA level. With HLXB9, PRM1, DICER and E2F1 as examples, we here show a range of situations that can occur when investigating gene expression, and give recommendations for the complementary methods that can verify gene expression data from large-scale studies, as well as give new information regarding the regulation of specific genes. Especially, we show that the absence of a protein despite high expression of the corresponding mRNA can be caused by expression of miRNAs targeting the mRNA. Additionally, we show through cloning the presence of both known and new miRNAs in the testis emphasizing the necessity for following up mRNA expression data by investigating expression at the protein level.

U2 - 10.1111/j.1365-2605.2007.00773.x

DO - 10.1111/j.1365-2605.2007.00773.x

M3 - Journal article

C2 - 17573847

SN - 0105-6263

VL - 30

SP - 316

EP - 327

JO - International Journal of Andrology

JF - International Journal of Andrology

IS - 4

ER -