TY - JOUR
T1 - Analysis of acid-soluble glucogenin pork extracts of two PRKAG3 genotypes by 1H liquid-state NMR spectroscopy and biochemical methods
AU - Larsen, Flemming Hofmann
AU - Essén-Gustavsson, Birgitta
AU - Jensen-Waern, Marianne
AU - Lametsch, Rene
AU - Karlsson, Anders H
AU - Lindahl, Gunilla Karin
PY - 2011/11/23
Y1 - 2011/11/23
N2 - Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN- and rn+ genotype) were analyzed by both 1H liquid-state NMR spectroscopy and a biochemical method. The 1H NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of α-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different post-mortem glycogen degradation mechanisms in the two genotypes.
AB - Meat extracts with acid-soluble glycogen (macroglycogen) from M. longissmus dorsi of carriers and noncarriers of the PRKAG3 mutation (RN- and rn+ genotype) were analyzed by both 1H liquid-state NMR spectroscopy and a biochemical method. The 1H NMR analysis revealed that shorter polymers (dimers, trimers, etc.) of α-1,4-linked glucose were generated 24-48 h post-mortem. This is not possible to elucidate with the biochemical method, by which only the total amount of hydrolyzed glucose residues is determined. The shorter polymers were primarily formed in carriers of the PRKAG3 mutation, suggesting different post-mortem glycogen degradation mechanisms in the two genotypes.
U2 - 10.1021/jf201822p
DO - 10.1021/jf201822p
M3 - Journal article
SN - 0021-8561
VL - 59
SP - 11895
EP - 11902
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 22
ER -