TY - JOUR
T1 - Analyses of Aloe polysaccharides using carbohydrate microarray profiling
AU - Isager Ahl, Louise
AU - Grace, Olwen M.
AU - Pedersen, Henriette Lodberg
AU - Willats, William George Tycho
AU - Jørgensen, Bodil
AU - Rønsted, Nina
PY - 2018/11/1
Y1 - 2018/11/1
N2 -
Background: As the popularity of
Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing. Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of
A. vera, and they have been widely studied in the past decades. However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.
Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.
Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.
Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue. Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.
Conclusions: These preliminary data suggest that the polysaccharides vary between species and that true species of Aloe may differ from segregate genera.
AB -
Background: As the popularity of
Aloe vera extracts continues to rise, a desire to fully understand the individual polymer components of the leaf mesophyll, their relation to one another, and the effects they have on the human body are increasing. Polysaccharides present in the leaf mesophyll have been identified as the components responsible for the biological activities of
A. vera, and they have been widely studied in the past decades. However, the commonly used methods do not provide the desired platform to conduct large comparative studies of polysaccharide compositions, as most of them require a complete or near-complete fractionation of the polymers.
Objective: The objective for this study was to assess whether carbohydrate microarrays could be used for the high-throughput analysis of cell wall polysaccharides in aloe leaf mesophyll.
Methods: The method we chose is known as comprehensive microarray polymer profiling (CoMPP) and combines the high-throughput capacity of microarray technology with the specificity of molecular probes.
Results: Preliminary findings showed that CoMPP can successfully be used for high-throughput screening of aloe leaf mesophyll tissue. Seventeen species of Aloe and closely related genera were analyzed, and a clear difference in the polysaccharide compositions of the mesophyll tissues was seen.
Conclusions: These preliminary data suggest that the polysaccharides vary between species and that true species of Aloe may differ from segregate genera.
UR - http://www.scopus.com/inward/record.url?scp=85055618632&partnerID=8YFLogxK
U2 - 10.5740/jaoacint.18-0120
DO - 10.5740/jaoacint.18-0120
M3 - Journal article
C2 - 29895348
SN - 1060-3271
VL - 101
SP - 1720
EP - 1728
JO - Journal of AOAC International
JF - Journal of AOAC International
IS - 6
ER -