An original phylogenetic approach identified mitochondrial haplogroup T1a1 as inversely associated with breast cancer risk in BRCA2 mutation carriers

Sophie Blein; Claire Bardel; Vincent Danjean; Lesley McGuffog; Sue Healey; Daniel Barrowdale; Andrew Lee; Joe Dennis; Karoline B Kuchenbaecker; Penny Soucy; Mary Beth Terry; Wendy K Chung; David E Goldgar; Saundra S Buys; Ramunas Janavicius; Laima Tihomirova; Nadine Tung; Cecilia M Dorfling; Elizabeth J van Rensburg; Susan L Neuhausen; Yuan Chun Ding; Anne-Marie Gerdes; Bent Ejlertsen; Finn C Nielsen; Thomas VO Hansen; Ana Osorio; Javier Benitez; Raquel Andrés Conejero; Ena Segota; Jeffrey N Weitzel; Margo Thelander; Paolo Peterlongo; Paolo Radice; Valeria Pensotti; Riccardo Dolcetti; Bernardo Bonanni; Bernard Peissel; Daniela Zaffaroni; Giulietta Scuvera; Siranoush Manoukian; Liliana Varesco; Gabriele L Capone; Laura Papi; Laura Ottini; Drakoulis Yannoukakos; Irene Konstantopoulou; Judy Garber; Ute Hamann; Alan Donaldson; Angela Brady; Breast Cancer Family Registry

doi:10.1186/s13058-015-0567-2

An original phylogenetic approach identified mitochondrial haplogroup T1a1 as inversely associated with breast cancer risk in BRCA2 mutation carriers

Sophie Blein, Claire Bardel, Vincent Danjean, Lesley McGuffog, Sue Healey, Daniel Barrowdale, Andrew Lee, Joe Dennis, Karoline B Kuchenbaecker, Penny Soucy, Mary Beth Terry, Wendy K Chung, David E Goldgar, Saundra S Buys, Ramunas Janavicius, Laima Tihomirova, Nadine Tung, Cecilia M Dorfling, Elizabeth J van Rensburg, Susan L NeuhausenYuan Chun Ding, Anne-Marie Gerdes, Bent Ejlertsen, Finn C Nielsen, Thomas VO Hansen, Ana Osorio, Javier Benitez, Raquel Andrés Conejero, Ena Segota, Jeffrey N Weitzel, Margo Thelander, Paolo Peterlongo, Paolo Radice, Valeria Pensotti, Riccardo Dolcetti, Bernardo Bonanni, Bernard Peissel, Daniela Zaffaroni, Giulietta Scuvera, Siranoush Manoukian, Liliana Varesco, Gabriele L Capone, Laura Papi, Laura Ottini, Drakoulis Yannoukakos, Irene Konstantopoulou, Judy Garber, Ute Hamann, Alan Donaldson, Angela Brady, Breast Cancer Family Registry

Department of Clinical Medicine

20 Citations (Scopus)

Abstract

INTRODUCTION: Individuals carrying pathogenic mutations in the BRCA1 and BRCA2 genes have a high lifetime risk of breast cancer. BRCA1 and BRCA2 are involved in DNA double-strand break repair, DNA alterations that can be caused by exposure to reactive oxygen species, a main source of which are mitochondria. Mitochondrial genome variations affect electron transport chain efficiency and reactive oxygen species production. Individuals with different mitochondrial haplogroups differ in their metabolism and sensitivity to oxidative stress. Variability in mitochondrial genetic background can alter reactive oxygen species production, leading to cancer risk. In the present study, we tested the hypothesis that mitochondrial haplogroups modify breast cancer risk in BRCA1/2 mutation carriers.

METHODS: We genotyped 22,214 (11,421 affected, 10,793 unaffected) mutation carriers belonging to the Consortium of Investigators of Modifiers of BRCA1/2 for 129 mitochondrial polymorphisms using the iCOGS array. Haplogroup inference and association detection were performed using a phylogenetic approach. ALTree was applied to explore the reference mitochondrial evolutionary tree and detect subclades enriched in affected or unaffected individuals.

RESULTS: We discovered that subclade T1a1 was depleted in affected BRCA2 mutation carriers compared with the rest of clade T (hazard ratio (HR) = 0.55; 95% confidence interval (CI), 0.34 to 0.88; P = 0.01). Compared with the most frequent haplogroup in the general population (that is, H and T clades), the T1a1 haplogroup has a HR of 0.62 (95% CI, 0.40 to 0.95; P = 0.03). We also identified three potential susceptibility loci, including G13708A/rs28359178, which has demonstrated an inverse association with familial breast cancer risk.

CONCLUSIONS: This study illustrates how original approaches such as the phylogeny-based method we used can empower classical molecular epidemiological studies aimed at identifying association or risk modification effects.

Original language	English
Article number	61
Journal	Breast cancer research : BCR
Volume	17
Issue number	1
Pages (from-to)	1-15
Number of pages	15
ISSN	1465-5411
DOIs	https://doi.org/10.1186/s13058-015-0567-2
Publication status	Published - 25 Apr 2015

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Blein, S., Bardel, C., Danjean, V., McGuffog, L., Healey, S., Barrowdale, D., Lee, A., Dennis, J., Kuchenbaecker, K. B., Soucy, P., Terry, M. B., Chung, W. K., Goldgar, D. E., Buys, S. S., Janavicius, R., Tihomirova, L., Tung, N., Dorfling, C. M., van Rensburg, E. J., ... Breast Cancer Family Registry (2015). An original phylogenetic approach identified mitochondrial haplogroup T1a1 as inversely associated with breast cancer risk in BRCA2 mutation carriers. Breast cancer research : BCR, 17(1), 1-15. Article 61. https://doi.org/10.1186/s13058-015-0567-2

An original phylogenetic approach identified mitochondrial haplogroup T1a1 as inversely associated with breast cancer risk in BRCA2 mutation carriers. / Blein, Sophie; Bardel, Claire; Danjean, Vincent et al.
In: Breast cancer research : BCR, Vol. 17, No. 1, 61, 25.04.2015, p. 1-15.

Research output: Contribution to journal › Journal article › Research › peer-review

Blein, S, Bardel, C, Danjean, V, McGuffog, L, Healey, S, Barrowdale, D, Lee, A, Dennis, J, Kuchenbaecker, KB, Soucy, P, Terry, MB, Chung, WK, Goldgar, DE, Buys, SS, Janavicius, R, Tihomirova, L, Tung, N, Dorfling, CM, van Rensburg, EJ, Neuhausen, SL, Ding, YC, Gerdes, A-M, Ejlertsen, B, Nielsen, FC, Hansen, TVO, Osorio, A, Benitez, J, Conejero, RA, Segota, E, Weitzel, JN, Thelander, M, Peterlongo, P, Radice, P, Pensotti, V, Dolcetti, R, Bonanni, B, Peissel, B, Zaffaroni, D, Scuvera, G, Manoukian, S, Varesco, L, Capone, GL, Papi, L, Ottini, L, Yannoukakos, D, Konstantopoulou, I, Garber, J, Hamann, U, Donaldson, A, Brady, A & Breast Cancer Family Registry 2015, 'An original phylogenetic approach identified mitochondrial haplogroup T1a1 as inversely associated with breast cancer risk in BRCA2 mutation carriers', Breast cancer research : BCR, vol. 17, no. 1, 61, pp. 1-15. https://doi.org/10.1186/s13058-015-0567-2

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title = "An original phylogenetic approach identified mitochondrial haplogroup T1a1 as inversely associated with breast cancer risk in BRCA2 mutation carriers",

abstract = "INTRODUCTION: Individuals carrying pathogenic mutations in the BRCA1 and BRCA2 genes have a high lifetime risk of breast cancer. BRCA1 and BRCA2 are involved in DNA double-strand break repair, DNA alterations that can be caused by exposure to reactive oxygen species, a main source of which are mitochondria. Mitochondrial genome variations affect electron transport chain efficiency and reactive oxygen species production. Individuals with different mitochondrial haplogroups differ in their metabolism and sensitivity to oxidative stress. Variability in mitochondrial genetic background can alter reactive oxygen species production, leading to cancer risk. In the present study, we tested the hypothesis that mitochondrial haplogroups modify breast cancer risk in BRCA1/2 mutation carriers.METHODS: We genotyped 22,214 (11,421 affected, 10,793 unaffected) mutation carriers belonging to the Consortium of Investigators of Modifiers of BRCA1/2 for 129 mitochondrial polymorphisms using the iCOGS array. Haplogroup inference and association detection were performed using a phylogenetic approach. ALTree was applied to explore the reference mitochondrial evolutionary tree and detect subclades enriched in affected or unaffected individuals.RESULTS: We discovered that subclade T1a1 was depleted in affected BRCA2 mutation carriers compared with the rest of clade T (hazard ratio (HR) = 0.55; 95% confidence interval (CI), 0.34 to 0.88; P = 0.01). Compared with the most frequent haplogroup in the general population (that is, H and T clades), the T1a1 haplogroup has a HR of 0.62 (95% CI, 0.40 to 0.95; P = 0.03). We also identified three potential susceptibility loci, including G13708A/rs28359178, which has demonstrated an inverse association with familial breast cancer risk.CONCLUSIONS: This study illustrates how original approaches such as the phylogeny-based method we used can empower classical molecular epidemiological studies aimed at identifying association or risk modification effects.",

author = "Sophie Blein and Claire Bardel and Vincent Danjean and Lesley McGuffog and Sue Healey and Daniel Barrowdale and Andrew Lee and Joe Dennis and Kuchenbaecker, {Karoline B} and Penny Soucy and Terry, {Mary Beth} and Chung, {Wendy K} and Goldgar, {David E} and Buys, {Saundra S} and Ramunas Janavicius and Laima Tihomirova and Nadine Tung and Dorfling, {Cecilia M} and {van Rensburg}, {Elizabeth J} and Neuhausen, {Susan L} and Ding, {Yuan Chun} and Anne-Marie Gerdes and Bent Ejlertsen and Nielsen, {Finn C} and Hansen, {Thomas VO} and Ana Osorio and Javier Benitez and Conejero, {Raquel Andr{\'e}s} and Ena Segota and Weitzel, {Jeffrey N} and Margo Thelander and Paolo Peterlongo and Paolo Radice and Valeria Pensotti and Riccardo Dolcetti and Bernardo Bonanni and Bernard Peissel and Daniela Zaffaroni and Giulietta Scuvera and Siranoush Manoukian and Liliana Varesco and Capone, {Gabriele L} and Laura Papi and Laura Ottini and Drakoulis Yannoukakos and Irene Konstantopoulou and Judy Garber and Ute Hamann and Alan Donaldson and Angela Brady and {Breast Cancer Family Registry}",

year = "2015",

month = apr,

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doi = "10.1186/s13058-015-0567-2",

language = "English",

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journal = "Breast cancer research : BCR",

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T1 - An original phylogenetic approach identified mitochondrial haplogroup T1a1 as inversely associated with breast cancer risk in BRCA2 mutation carriers

AU - Blein, Sophie

AU - Bardel, Claire

AU - Danjean, Vincent

AU - McGuffog, Lesley

AU - Healey, Sue

AU - Barrowdale, Daniel

AU - Lee, Andrew

AU - Dennis, Joe

AU - Kuchenbaecker, Karoline B

AU - Soucy, Penny

AU - Terry, Mary Beth

AU - Chung, Wendy K

AU - Goldgar, David E

AU - Buys, Saundra S

AU - Janavicius, Ramunas

AU - Tihomirova, Laima

AU - Tung, Nadine

AU - Dorfling, Cecilia M

AU - van Rensburg, Elizabeth J

AU - Neuhausen, Susan L

AU - Ding, Yuan Chun

AU - Gerdes, Anne-Marie

AU - Ejlertsen, Bent

AU - Nielsen, Finn C

AU - Hansen, Thomas VO

AU - Osorio, Ana

AU - Benitez, Javier

AU - Conejero, Raquel Andrés

AU - Segota, Ena

AU - Weitzel, Jeffrey N

AU - Thelander, Margo

AU - Peterlongo, Paolo

AU - Radice, Paolo

AU - Pensotti, Valeria

AU - Dolcetti, Riccardo

AU - Bonanni, Bernardo

AU - Peissel, Bernard

AU - Zaffaroni, Daniela

AU - Scuvera, Giulietta

AU - Manoukian, Siranoush

AU - Varesco, Liliana

AU - Capone, Gabriele L

AU - Papi, Laura

AU - Ottini, Laura

AU - Yannoukakos, Drakoulis

AU - Konstantopoulou, Irene

AU - Garber, Judy

AU - Hamann, Ute

AU - Donaldson, Alan

AU - Brady, Angela

AU - Breast Cancer Family Registry

PY - 2015/4/25

Y1 - 2015/4/25

N2 - INTRODUCTION: Individuals carrying pathogenic mutations in the BRCA1 and BRCA2 genes have a high lifetime risk of breast cancer. BRCA1 and BRCA2 are involved in DNA double-strand break repair, DNA alterations that can be caused by exposure to reactive oxygen species, a main source of which are mitochondria. Mitochondrial genome variations affect electron transport chain efficiency and reactive oxygen species production. Individuals with different mitochondrial haplogroups differ in their metabolism and sensitivity to oxidative stress. Variability in mitochondrial genetic background can alter reactive oxygen species production, leading to cancer risk. In the present study, we tested the hypothesis that mitochondrial haplogroups modify breast cancer risk in BRCA1/2 mutation carriers.METHODS: We genotyped 22,214 (11,421 affected, 10,793 unaffected) mutation carriers belonging to the Consortium of Investigators of Modifiers of BRCA1/2 for 129 mitochondrial polymorphisms using the iCOGS array. Haplogroup inference and association detection were performed using a phylogenetic approach. ALTree was applied to explore the reference mitochondrial evolutionary tree and detect subclades enriched in affected or unaffected individuals.RESULTS: We discovered that subclade T1a1 was depleted in affected BRCA2 mutation carriers compared with the rest of clade T (hazard ratio (HR) = 0.55; 95% confidence interval (CI), 0.34 to 0.88; P = 0.01). Compared with the most frequent haplogroup in the general population (that is, H and T clades), the T1a1 haplogroup has a HR of 0.62 (95% CI, 0.40 to 0.95; P = 0.03). We also identified three potential susceptibility loci, including G13708A/rs28359178, which has demonstrated an inverse association with familial breast cancer risk.CONCLUSIONS: This study illustrates how original approaches such as the phylogeny-based method we used can empower classical molecular epidemiological studies aimed at identifying association or risk modification effects.

AB - INTRODUCTION: Individuals carrying pathogenic mutations in the BRCA1 and BRCA2 genes have a high lifetime risk of breast cancer. BRCA1 and BRCA2 are involved in DNA double-strand break repair, DNA alterations that can be caused by exposure to reactive oxygen species, a main source of which are mitochondria. Mitochondrial genome variations affect electron transport chain efficiency and reactive oxygen species production. Individuals with different mitochondrial haplogroups differ in their metabolism and sensitivity to oxidative stress. Variability in mitochondrial genetic background can alter reactive oxygen species production, leading to cancer risk. In the present study, we tested the hypothesis that mitochondrial haplogroups modify breast cancer risk in BRCA1/2 mutation carriers.METHODS: We genotyped 22,214 (11,421 affected, 10,793 unaffected) mutation carriers belonging to the Consortium of Investigators of Modifiers of BRCA1/2 for 129 mitochondrial polymorphisms using the iCOGS array. Haplogroup inference and association detection were performed using a phylogenetic approach. ALTree was applied to explore the reference mitochondrial evolutionary tree and detect subclades enriched in affected or unaffected individuals.RESULTS: We discovered that subclade T1a1 was depleted in affected BRCA2 mutation carriers compared with the rest of clade T (hazard ratio (HR) = 0.55; 95% confidence interval (CI), 0.34 to 0.88; P = 0.01). Compared with the most frequent haplogroup in the general population (that is, H and T clades), the T1a1 haplogroup has a HR of 0.62 (95% CI, 0.40 to 0.95; P = 0.03). We also identified three potential susceptibility loci, including G13708A/rs28359178, which has demonstrated an inverse association with familial breast cancer risk.CONCLUSIONS: This study illustrates how original approaches such as the phylogeny-based method we used can empower classical molecular epidemiological studies aimed at identifying association or risk modification effects.

U2 - 10.1186/s13058-015-0567-2

DO - 10.1186/s13058-015-0567-2

M3 - Journal article

C2 - 25925750

SN - 1465-5411

VL - 17

SP - 1

EP - 15

JO - Breast cancer research : BCR

JF - Breast cancer research : BCR

IS - 1

M1 - 61

ER -

An original phylogenetic approach identified mitochondrial haplogroup T1a1 as inversely associated with breast cancer risk in BRCA2 mutation carriers

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