An online resource for GPCR structure determination and analysis

Christian Munk; Eshita Mutt; Vignir Isberg; Louise F Nikolajsen; Janne M Bibbe; Tilman Flock; Michael A Hanson; Raymond C Stevens; Xavier Deupi; David E Gloriam

doi:10.1038/s41592-018-0302-x

An online resource for GPCR structure determination and analysis

Christian Munk, Eshita Mutt, Vignir Isberg, Louise F Nikolajsen, Janne M Bibbe, Tilman Flock, Michael A Hanson, Raymond C Stevens, Xavier Deupi, David E Gloriam

53 Citations (Scopus)

Abstract

G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated in GPCRdb ( http://www.gpcrdb.org ) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies.

Original language	English
Journal	Nature Methods
Volume	16
Issue number	2
Pages (from-to)	151-162
Number of pages	12
ISSN	1548-7091
DOIs	https://doi.org/10.1038/s41592-018-0302-x
Publication status	Published - 1 Feb 2019

Keywords

Allosteric Site
Animals
Cattle
Computational Biology/methods
Cryoelectron Microscopy
Crystallography, X-Ray
Databases, Protein
Drug Design
Glycosylation
HEK293 Cells
Humans
Internet
Mutation
Phosphorylation
Protein Domains
Protein Engineering
Receptors, G-Protein-Coupled/genetics
Rhodopsin/chemistry
Signal Transduction
Software

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10.1038/s41592-018-0302-x

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abstract = "G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated in GPCRdb ( http://www.gpcrdb.org ) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies.",

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AU - Munk, Christian

AU - Mutt, Eshita

AU - Isberg, Vignir

AU - Nikolajsen, Louise F

AU - Bibbe, Janne M

AU - Flock, Tilman

AU - Hanson, Michael A

AU - Stevens, Raymond C

AU - Deupi, Xavier

AU - Gloriam, David E

PY - 2019/2/1

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N2 - G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated in GPCRdb ( http://www.gpcrdb.org ) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies.

AB - G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated in GPCRdb ( http://www.gpcrdb.org ) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies.

KW - Allosteric Site

KW - Animals

KW - Cattle

KW - Computational Biology/methods

KW - Cryoelectron Microscopy

KW - Crystallography, X-Ray

KW - Databases, Protein

KW - Drug Design

KW - Glycosylation

KW - HEK293 Cells

KW - Humans

KW - Internet

KW - Mutation

KW - Phosphorylation

KW - Protein Domains

KW - Protein Engineering

KW - Receptors, G-Protein-Coupled/genetics

KW - Rhodopsin/chemistry

KW - Signal Transduction

KW - Software

U2 - 10.1038/s41592-018-0302-x

DO - 10.1038/s41592-018-0302-x

M3 - Review

C2 - 30664776

SN - 1548-7091

VL - 16

SP - 151

EP - 162

JO - Nature Methods

JF - Nature Methods

IS - 2

ER -

An online resource for GPCR structure determination and analysis

Abstract

Keywords

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