An NPF transporter exports a central monoterpene indole alkaloid intermediate from the vacuole

Richard Payne; Deyang Xu; Emilien Foureau; Marta  Ines Soares Teto Carqueijeiro; Audrey Oudin; Thomas Dugé de Bernonville; Vlastimil Novák; Meike Burow; Carl Erik Olsen; D. Marc  Jones; Evangelos C. Tatsis; Ali Pendleton; Barbara Ann Halkier; Fernando Geu-Flores; Vincent Courdavault; Hussam Hassan Nour-Eldin; Sarah E O'Connor

doi:10.1038/nplants.2016.208

An NPF transporter exports a central monoterpene indole alkaloid intermediate from the vacuole

Richard Payne, Deyang Xu, Emilien Foureau, Marta Ines Soares Teto Carqueijeiro, Audrey Oudin, Thomas Dugé de Bernonville, Vlastimil Novák, Meike Burow, Carl Erik Olsen, D. Marc Jones, Evangelos C. Tatsis, Ali Pendleton, Barbara Ann Halkier, Fernando Geu-Flores, Vincent Courdavault, Hussam Hassan Nour-Eldin, Sarah E O'Connor

48 Citations (Scopus)

Abstract

Plants sequester intermediates of metabolic pathways into different cellular compartments, but the mechanisms by which these molecules are transported remain poorly understood. Monoterpene indole alkaloids, a class of specialized metabolites that includes the anticancer agent vincristine, antimalarial quinine and neurotoxin strychnine, are synthesized in several different cellular locations. However, the transporters that control the movement of these biosynthetic intermediates within cellular compartments have not been discovered. Here we present the discovery of a tonoplast localized nitrate/peptide family (NPF) transporter from Catharanthus roseus, CrNPF2.9, that exports strictosidine, the central intermediate of this pathway, into the cytosol from the vacuole. This discovery highlights the role that intracellular localization plays in specialized metabolism, and sets the stage for understanding and controlling the central branch point of this pharmacologically important group of compounds.

Original language	English
Article number	16208
Journal	Nature Plants
Volume	3
Number of pages	9
ISSN	2055-0278
DOIs	https://doi.org/10.1038/nplants.2016.208
Publication status	Published - 13 Jan 2017

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Payne, R., Xu, D., Foureau, E., Ines Soares Teto Carqueijeiro, M., Oudin, A., Dugé de Bernonville, T., Novák, V., Burow, M., Olsen, C. E., Jones, D. M., C. Tatsis, E., Pendleton, A., Halkier, B. A., Geu-Flores, F., Courdavault, V., Nour-Eldin, H. H., & O'Connor, S. E. (2017). An NPF transporter exports a central monoterpene indole alkaloid intermediate from the vacuole. Nature Plants, 3, Article 16208. https://doi.org/10.1038/nplants.2016.208

Payne, R, Xu, D, Foureau, E, Ines Soares Teto Carqueijeiro, M, Oudin, A, Dugé de Bernonville, T, Novák, V, Burow, M, Olsen, CE, Jones, DM, C. Tatsis, E, Pendleton, A, Halkier, BA , Geu-Flores, F, Courdavault, V, Nour-Eldin, HH & O'Connor, SE 2017, 'An NPF transporter exports a central monoterpene indole alkaloid intermediate from the vacuole', Nature Plants, vol. 3, 16208. https://doi.org/10.1038/nplants.2016.208

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title = "An NPF transporter exports a central monoterpene indole alkaloid intermediate from the vacuole",

abstract = "Plants sequester intermediates of metabolic pathways into different cellular compartments, but the mechanisms by which these molecules are transported remain poorly understood. Monoterpene indole alkaloids, a class of specialized metabolites that includes the anticancer agent vincristine, antimalarial quinine and neurotoxin strychnine, are synthesized in several different cellular locations. However, the transporters that control the movement of these biosynthetic intermediates within cellular compartments have not been discovered. Here we present the discovery of a tonoplast localized nitrate/peptide family (NPF) transporter from Catharanthus roseus, CrNPF2.9, that exports strictosidine, the central intermediate of this pathway, into the cytosol from the vacuole. This discovery highlights the role that intracellular localization plays in specialized metabolism, and sets the stage for understanding and controlling the central branch point of this pharmacologically important group of compounds.",

author = "Richard Payne and Deyang Xu and Emilien Foureau and {Ines Soares Teto Carqueijeiro}, Marta and Audrey Oudin and {Dug{\'e} de Bernonville}, Thomas and Vlastimil Nov{\'a}k and Meike Burow and Olsen, {Carl Erik} and Jones, {D. Marc} and {C. Tatsis}, Evangelos and Ali Pendleton and Halkier, {Barbara Ann} and Fernando Geu-Flores and Vincent Courdavault and Nour-Eldin, {Hussam Hassan} and O'Connor, {Sarah E}",

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T1 - An NPF transporter exports a central monoterpene indole alkaloid intermediate from the vacuole

AU - Payne, Richard

AU - Xu, Deyang

AU - Foureau, Emilien

AU - Ines Soares Teto Carqueijeiro, Marta

AU - Oudin, Audrey

AU - Dugé de Bernonville, Thomas

AU - Novák, Vlastimil

AU - Burow, Meike

AU - Olsen, Carl Erik

AU - Jones, D. Marc

AU - C. Tatsis, Evangelos

AU - Pendleton, Ali

AU - Halkier, Barbara Ann

AU - Geu-Flores, Fernando

AU - Courdavault, Vincent

AU - Nour-Eldin, Hussam Hassan

AU - O'Connor, Sarah E

PY - 2017/1/13

Y1 - 2017/1/13

N2 - Plants sequester intermediates of metabolic pathways into different cellular compartments, but the mechanisms by which these molecules are transported remain poorly understood. Monoterpene indole alkaloids, a class of specialized metabolites that includes the anticancer agent vincristine, antimalarial quinine and neurotoxin strychnine, are synthesized in several different cellular locations. However, the transporters that control the movement of these biosynthetic intermediates within cellular compartments have not been discovered. Here we present the discovery of a tonoplast localized nitrate/peptide family (NPF) transporter from Catharanthus roseus, CrNPF2.9, that exports strictosidine, the central intermediate of this pathway, into the cytosol from the vacuole. This discovery highlights the role that intracellular localization plays in specialized metabolism, and sets the stage for understanding and controlling the central branch point of this pharmacologically important group of compounds.

AB - Plants sequester intermediates of metabolic pathways into different cellular compartments, but the mechanisms by which these molecules are transported remain poorly understood. Monoterpene indole alkaloids, a class of specialized metabolites that includes the anticancer agent vincristine, antimalarial quinine and neurotoxin strychnine, are synthesized in several different cellular locations. However, the transporters that control the movement of these biosynthetic intermediates within cellular compartments have not been discovered. Here we present the discovery of a tonoplast localized nitrate/peptide family (NPF) transporter from Catharanthus roseus, CrNPF2.9, that exports strictosidine, the central intermediate of this pathway, into the cytosol from the vacuole. This discovery highlights the role that intracellular localization plays in specialized metabolism, and sets the stage for understanding and controlling the central branch point of this pharmacologically important group of compounds.

U2 - 10.1038/nplants.2016.208

DO - 10.1038/nplants.2016.208

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SN - 2055-0278

VL - 3

JO - Nature Plants

JF - Nature Plants

M1 - 16208

ER -

An NPF transporter exports a central monoterpene indole alkaloid intermediate from the vacuole

Abstract

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