An intron in a ribosomal protein gene from Tetrahymena

Henrik Nielsen; Per Hove Andreasen; Hanne Dreisig; Karsten Kristiansen; Jan Engberg

An intron in a ribosomal protein gene from Tetrahymena

Henrik Nielsen, Per Hove Andreasen, Hanne Dreisig, Karsten Kristiansen, Jan Engberg

Abstract

We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.

Original language	English
Journal	EMBO Journal
Volume	5
Issue number	10
Pages (from-to)	2711-2717
Number of pages	6
ISSN	0261-4189
Publication status	Published - 1986
Externally published	Yes

Access to Document

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1167173

Cite this

@article{2f79576013c511de8478000ea68e967b,

title = "An intron in a ribosomal protein gene from Tetrahymena",

abstract = "We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.",

author = "Henrik Nielsen and Andreasen, {Per Hove} and Hanne Dreisig and Karsten Kristiansen and Jan Engberg",

year = "1986",

language = "English",

volume = "5",

pages = "2711--2717",

journal = "E M B O Journal",

issn = "0261-4189",

publisher = "Wiley-Blackwell",

number = "10",

}

TY - JOUR

T1 - An intron in a ribosomal protein gene from Tetrahymena

AU - Nielsen, Henrik

AU - Andreasen, Per Hove

AU - Dreisig, Hanne

AU - Kristiansen, Karsten

AU - Engberg, Jan

PY - 1986

Y1 - 1986

N2 - We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.

AB - We have cloned and sequenced a single copy gene encoding a ribosomal protein from the ciliate Tetrahymena thermophila. The gene product was identified as ribosomal protein S25 by comparison of the migration in two-dimensional polyacrylamide gels of the protein synthesized by translation in vitro of hybrid-selected mRNA and authentic ribosomal proteins. The proteins show strong homology to ribosomal protein S12 from Escherichia coli. The coding region of the gene is interrupted by a 979-bp intron 68 bp downstream of the translation start. This is the first intron in a protein encoding gene of a ciliate to be described at the nucleotide sequence level. The intron obeys the GT/AG rule for splice junctions of nuclear mRNA introns from higher eukaryotes but lacks the pyrimidine stretch usually found in the immediate vicinity of the 3' splice junction. The structure of the intron and the fact that it is found together with the well described self-splicing rRNA intron is discussed in relation to the evolution of RNA splicing.

M3 - Journal article

C2 - 16453715

SN - 0261-4189

VL - 5

SP - 2711

EP - 2717

JO - E M B O Journal

JF - E M B O Journal

IS - 10

ER -

An intron in a ribosomal protein gene from Tetrahymena

Abstract

Access to Document

Fingerprint

Cite this