An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium

Birger Brodin; K A Rytved; R Nielsen

An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium

18 Citations (Scopus)

Abstract

The aim of this study was to investigate the mechanisms by which increases in free cytosolic calcium ([Ca2+]i) cause a decrease in macroscopic sodium absorption across principal cells of the frog skin epithelium. [Ca2+]i was measured with fura-2 in an epifluorescence microscope set-up, sodium absorption was measured by the voltage-clamp technique and cellular potential was measured using microelectrodes. The endoplasmic reticulum calcium-ATPase inhibitor thapsigargin (0.4 microM) increased [Ca2+]i from 66 +/- 9 to 137 +/- 19 nM (n = 13, P = 0.002). Thapsigargin caused the amiloride-sensitive short circuit current (Isc) to drop from 26.4 to 10.6 microA cm-2 (n = 19, P

Original language	English
Journal	Pflügers Archiv - European Journal of Physiology
Volume	433
Issue number	1-2
Pages (from-to)	16-25
Number of pages	10
ISSN	0031-6768
Publication status	Published - 1996

Keywords

Animals
Biological Transport, Active
Calcium
Cell Membrane
Chloride Channels
Chlorides
Cytosol
Electrophysiology
Epithelium
Female
Intracellular Membranes
Male
Osmolar Concentration
Permeability
Potassium
Rana temporaria
Skin
Sodium
Sodium Channel Blockers
Thapsigargin

Cite this

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title = "An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium",

abstract = "The aim of this study was to investigate the mechanisms by which increases in free cytosolic calcium ([Ca2+]i) cause a decrease in macroscopic sodium absorption across principal cells of the frog skin epithelium. [Ca2+]i was measured with fura-2 in an epifluorescence microscope set-up, sodium absorption was measured by the voltage-clamp technique and cellular potential was measured using microelectrodes. The endoplasmic reticulum calcium-ATPase inhibitor thapsigargin (0.4 microM) increased [Ca2+]i from 66 +/- 9 to 137 +/- 19 nM (n = 13, P = 0.002). Thapsigargin caused the amiloride-sensitive short circuit current (Isc) to drop from 26.4 to 10.6 microA cm-2 (n = 19, P",

keywords = "Animals, Biological Transport, Active, Calcium, Cell Membrane, Chloride Channels, Chlorides, Cytosol, Electrophysiology, Epithelium, Female, Intracellular Membranes, Male, Osmolar Concentration, Permeability, Potassium, Rana temporaria, Skin, Sodium, Sodium Channel Blockers, Thapsigargin",

author = "Birger Brodin and Rytved, {K A} and R Nielsen",

year = "1996",

language = "English",

volume = "433",

pages = "16--25",

journal = "Pfl{\"u}gers Archiv - European Journal of Physiology",

issn = "0031-6768",

publisher = "Springer",

number = "1-2",

}

TY - JOUR

T1 - An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium

AU - Brodin, Birger

AU - Rytved, K A

AU - Nielsen, R

PY - 1996

Y1 - 1996

N2 - The aim of this study was to investigate the mechanisms by which increases in free cytosolic calcium ([Ca2+]i) cause a decrease in macroscopic sodium absorption across principal cells of the frog skin epithelium. [Ca2+]i was measured with fura-2 in an epifluorescence microscope set-up, sodium absorption was measured by the voltage-clamp technique and cellular potential was measured using microelectrodes. The endoplasmic reticulum calcium-ATPase inhibitor thapsigargin (0.4 microM) increased [Ca2+]i from 66 +/- 9 to 137 +/- 19 nM (n = 13, P = 0.002). Thapsigargin caused the amiloride-sensitive short circuit current (Isc) to drop from 26.4 to 10.6 microA cm-2 (n = 19, P

AB - The aim of this study was to investigate the mechanisms by which increases in free cytosolic calcium ([Ca2+]i) cause a decrease in macroscopic sodium absorption across principal cells of the frog skin epithelium. [Ca2+]i was measured with fura-2 in an epifluorescence microscope set-up, sodium absorption was measured by the voltage-clamp technique and cellular potential was measured using microelectrodes. The endoplasmic reticulum calcium-ATPase inhibitor thapsigargin (0.4 microM) increased [Ca2+]i from 66 +/- 9 to 137 +/- 19 nM (n = 13, P = 0.002). Thapsigargin caused the amiloride-sensitive short circuit current (Isc) to drop from 26.4 to 10.6 microA cm-2 (n = 19, P

KW - Animals

KW - Biological Transport, Active

KW - Calcium

KW - Cell Membrane

KW - Chloride Channels

KW - Chlorides

KW - Cytosol

KW - Electrophysiology

KW - Epithelium

KW - Female

KW - Intracellular Membranes

KW - Male

KW - Osmolar Concentration

KW - Permeability

KW - Potassium

KW - Rana temporaria

KW - Skin

KW - Sodium

KW - Sodium Channel Blockers

KW - Thapsigargin

M3 - Journal article

C2 - 9019717

SN - 0031-6768

VL - 433

SP - 16

EP - 25

JO - Pflügers Archiv - European Journal of Physiology

JF - Pflügers Archiv - European Journal of Physiology

IS - 1-2

ER -

An increase in [Ca2+]i activates basolateral chloride channels and inhibits apical sodium channels in frog skin epithelium

Abstract

Keywords

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