An assessment of the biotechnological use of hemoglobin modulation in cereals

Kim Hebelstrup; Jay K. Shah; Catherine Simpson; Jan Kofod Schjørring; Julien Mandon; Simona M. Cristescu; Frans J.M. Harren; Michael Wagner; Luis A J Mur; Abir U. Igamberdiev

doi:10.1111/ppl.12115

An assessment of the biotechnological use of hemoglobin modulation in cereals

Kim Hebelstrup, Jay K. Shah, Catherine Simpson, Jan Kofod Schjørring, Julien Mandon, Simona M. Cristescu, Frans J.M. Harren, Michael Wagner, Luis A J Mur, Abir U. Igamberdiev

Section for Plant and Soil Sciences

22 Citations (Scopus)

Abstract

Non-symbiotic hemoglobin (nsHb) genes are ubiquitous in plants, but their biological functions have mostly been studied in model plant species rather than in crops. nsHb influences cell signaling and metabolism by modulating the levels of nitric oxide (NO). Class 1 nsHb is upregulated under hypoxia and is involved in various biotic and abiotic stress responses. Ectopic overexpression of nsHb in Arabidopsis thaliana accelerates development, whilst targeted overexpression in seeds can increase seed yield. Such observations suggest that manipulating nsHb could be a valid biotechnological target. We studied the effects of overexpression of class 1 nsHb in the monocotyledonous crop plant barley (Hordeum vulgare cv. Golden Promise). nsHb was shown to be involved in NO metabolism in barley, as ectopic overexpression reduced the amount of NO released during hypoxia. Further, as in Arabidopsis, nsHb overexpression compromised basal resistance toward pathogens in barley. However, unlike Arabidopsis, nsHb ectopic overexpression delayed growth and development in barley, and seed specific overexpression reduced seed yield. Thus, nsHb overexpression in barley does not seem to be an efficient strategy for increasing yield in cereal crops. These findings highlight the necessity for using actual crop plants rather than laboratory model plants when assessing the effects of biotechnological approaches to crop improvement.

Original language	English
Journal	Physiologia Plantarum
Volume	150
Issue number	4
Pages (from-to)	593-603
Number of pages	11
ISSN	0031-9317
DOIs	https://doi.org/10.1111/ppl.12115
Publication status	Published - 2014

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title = "An assessment of the biotechnological use of hemoglobin modulation in cereals",

abstract = "Non-symbiotic hemoglobin (nsHb) genes are ubiquitous in plants, but their biological functions have mostly been studied in model plant species rather than in crops. nsHb influences cell signaling and metabolism by modulating the levels of nitric oxide (NO). Class 1 nsHb is upregulated under hypoxia and is involved in various biotic and abiotic stress responses. Ectopic overexpression of nsHb in Arabidopsis thaliana accelerates development, whilst targeted overexpression in seeds can increase seed yield. Such observations suggest that manipulating nsHb could be a valid biotechnological target. We studied the effects of overexpression of class 1 nsHb in the monocotyledonous crop plant barley (Hordeum vulgare cv. Golden Promise). nsHb was shown to be involved in NO metabolism in barley, as ectopic overexpression reduced the amount of NO released during hypoxia. Further, as in Arabidopsis, nsHb overexpression compromised basal resistance toward pathogens in barley. However, unlike Arabidopsis, nsHb ectopic overexpression delayed growth and development in barley, and seed specific overexpression reduced seed yield. Thus, nsHb overexpression in barley does not seem to be an efficient strategy for increasing yield in cereal crops. These findings highlight the necessity for using actual crop plants rather than laboratory model plants when assessing the effects of biotechnological approaches to crop improvement.",

author = "Kim Hebelstrup and Shah, {Jay K.} and Catherine Simpson and Schj{\o}rring, {Jan Kofod} and Julien Mandon and Cristescu, {Simona M.} and Harren, {Frans J.M.} and Michael Wagner and Mur, {Luis A J} and Igamberdiev, {Abir U.}",

year = "2014",

doi = "10.1111/ppl.12115",

language = "English",

volume = "150",

pages = "593--603",

journal = "Physiologia Plantarum",

issn = "0031-9317",

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TY - JOUR

T1 - An assessment of the biotechnological use of hemoglobin modulation in cereals

AU - Hebelstrup, Kim

AU - Shah, Jay K.

AU - Simpson, Catherine

AU - Schjørring, Jan Kofod

AU - Mandon, Julien

AU - Cristescu, Simona M.

AU - Harren, Frans J.M.

AU - Wagner, Michael

AU - Mur, Luis A J

AU - Igamberdiev, Abir U.

PY - 2014

Y1 - 2014

N2 - Non-symbiotic hemoglobin (nsHb) genes are ubiquitous in plants, but their biological functions have mostly been studied in model plant species rather than in crops. nsHb influences cell signaling and metabolism by modulating the levels of nitric oxide (NO). Class 1 nsHb is upregulated under hypoxia and is involved in various biotic and abiotic stress responses. Ectopic overexpression of nsHb in Arabidopsis thaliana accelerates development, whilst targeted overexpression in seeds can increase seed yield. Such observations suggest that manipulating nsHb could be a valid biotechnological target. We studied the effects of overexpression of class 1 nsHb in the monocotyledonous crop plant barley (Hordeum vulgare cv. Golden Promise). nsHb was shown to be involved in NO metabolism in barley, as ectopic overexpression reduced the amount of NO released during hypoxia. Further, as in Arabidopsis, nsHb overexpression compromised basal resistance toward pathogens in barley. However, unlike Arabidopsis, nsHb ectopic overexpression delayed growth and development in barley, and seed specific overexpression reduced seed yield. Thus, nsHb overexpression in barley does not seem to be an efficient strategy for increasing yield in cereal crops. These findings highlight the necessity for using actual crop plants rather than laboratory model plants when assessing the effects of biotechnological approaches to crop improvement.

AB - Non-symbiotic hemoglobin (nsHb) genes are ubiquitous in plants, but their biological functions have mostly been studied in model plant species rather than in crops. nsHb influences cell signaling and metabolism by modulating the levels of nitric oxide (NO). Class 1 nsHb is upregulated under hypoxia and is involved in various biotic and abiotic stress responses. Ectopic overexpression of nsHb in Arabidopsis thaliana accelerates development, whilst targeted overexpression in seeds can increase seed yield. Such observations suggest that manipulating nsHb could be a valid biotechnological target. We studied the effects of overexpression of class 1 nsHb in the monocotyledonous crop plant barley (Hordeum vulgare cv. Golden Promise). nsHb was shown to be involved in NO metabolism in barley, as ectopic overexpression reduced the amount of NO released during hypoxia. Further, as in Arabidopsis, nsHb overexpression compromised basal resistance toward pathogens in barley. However, unlike Arabidopsis, nsHb ectopic overexpression delayed growth and development in barley, and seed specific overexpression reduced seed yield. Thus, nsHb overexpression in barley does not seem to be an efficient strategy for increasing yield in cereal crops. These findings highlight the necessity for using actual crop plants rather than laboratory model plants when assessing the effects of biotechnological approaches to crop improvement.

U2 - 10.1111/ppl.12115

DO - 10.1111/ppl.12115

M3 - Journal article

AN - SCOPUS:84895927372

SN - 0031-9317

VL - 150

SP - 593

EP - 603

JO - Physiologia Plantarum

JF - Physiologia Plantarum

IS - 4

ER -

An assessment of the biotechnological use of hemoglobin modulation in cereals

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