Amylopectin chain length dynamics and activity signatures of key carbon metabolic enzymes highlight early maturation as culprit for yield reduction of barley endosperm starch after heat stress

Jose Antonio Cuesta-Seijo; Alice Jara De Porcellinis; Angela Hørdum Valente; Alexander Striebeck; Cynthia Voss; Lucia Marri; Andreas Hansson; Anita M Jansson; Malene Hessellund Dinesen; Jonatan Ulrik Fangel; Jesper Harholt; Milan Popovic; Mercedes Thieme; Anton Hochmuth; Samuel C Zeeman; Teis Nørgaard Mikkelsen; Rikke Bagger Jørgensen; Thomas Georg Roitsch; Birger Lindberg Møller; Ilka Braumann

doi:10.1093/pcp/pcz155

Amylopectin chain length dynamics and activity signatures of key carbon metabolic enzymes highlight early maturation as culprit for yield reduction of barley endosperm starch after heat stress

Jose Antonio Cuesta-Seijo, Alice Jara De Porcellinis, Angela Hørdum Valente, Alexander Striebeck, Cynthia Voss, Lucia Marri, Andreas Hansson, Anita M Jansson, Malene Hessellund Dinesen, Jonatan Ulrik Fangel, Jesper Harholt, Milan Popovic, Mercedes Thieme, Anton Hochmuth, Samuel C Zeeman, Teis Nørgaard Mikkelsen, Rikke Bagger Jørgensen, Thomas Georg Roitsch, Birger Lindberg Møller, Ilka Braumann

3 Citations (Scopus)

Abstract

Abiotic environmental stresses have a negative impact on the yield and quality of crops. Understanding these stresses is an essential enabler for mitigating breeding strategies and it becomes more important as the frequency of extreme weather conditions increases due to climate change. This study analyses the response of barley (Hordeum vulgare L.) to a heat wave during grain filling in three distinct stages: the heat wave itself, the return to a normal temperature regime, and the process of maturation and desiccation. The properties and structure of the starch produced were followed throughout the maturational stages. Furthermore, the key enzymes involved in the carbohydrate supply to the grain were monitored. We observed differences in starch structure with well-separated effects because of heat stress and during senescence. Heat stress produced marked effects on sucrolytic enzymes in source and sink tissues. Early cessation of plant development as an indirect consequence of the heat wave was identified as the major contributor to final yield loss from the stress, highlighting the importance for functional stay-green traits for the development of heat-resistant cereals.

Original language	English
Journal	Plant and Cell Physiology
ISSN	0032-0781
DOIs	https://doi.org/10.1093/pcp/pcz155
Publication status	Published - 1 Dec 2019

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10.1093/pcp/pcz155Licence: CC BY

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Cuesta-Seijo, J. A., De Porcellinis, A. J., Valente, A. H., Striebeck, A., Voss, C., Marri, L., Hansson, A., Jansson, A. M., Dinesen, M. H., Fangel, J. U., Harholt, J., Popovic, M., Thieme, M., Hochmuth, A., Zeeman, S. C., Mikkelsen, T. N., Jørgensen, R. B., Roitsch, T. G., Møller, B. L., & Braumann, I. (2019). Amylopectin chain length dynamics and activity signatures of key carbon metabolic enzymes highlight early maturation as culprit for yield reduction of barley endosperm starch after heat stress. Plant and Cell Physiology. https://doi.org/10.1093/pcp/pcz155

Amylopectin chain length dynamics and activity signatures of key carbon metabolic enzymes highlight early maturation as culprit for yield reduction of barley endosperm starch after heat stress. / Cuesta-Seijo, Jose Antonio; De Porcellinis, Alice Jara; Valente, Angela Hørdum et al.

In: Plant and Cell Physiology, 01.12.2019.

Research output: Contribution to journal › Journal article › Research › peer-review

Cuesta-Seijo, JA, De Porcellinis, AJ, Valente, AH, Striebeck, A, Voss, C, Marri, L, Hansson, A, Jansson, AM, Dinesen, MH, Fangel, JU, Harholt, J, Popovic, M, Thieme, M, Hochmuth, A, Zeeman, SC, Mikkelsen, TN, Jørgensen, RB, Roitsch, TG , Møller, BL & Braumann, I 2019, 'Amylopectin chain length dynamics and activity signatures of key carbon metabolic enzymes highlight early maturation as culprit for yield reduction of barley endosperm starch after heat stress', Plant and Cell Physiology. https://doi.org/10.1093/pcp/pcz155

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title = "Amylopectin chain length dynamics and activity signatures of key carbon metabolic enzymes highlight early maturation as culprit for yield reduction of barley endosperm starch after heat stress",

abstract = "Abiotic environmental stresses have a negative impact on the yield and quality of crops. Understanding these stresses is an essential enabler for mitigating breeding strategies and it becomes more important as the frequency of extreme weather conditions increases due to climate change. This study analyses the response of barley (Hordeum vulgare L.) to a heat wave during grain filling in three distinct stages: the heat wave itself, the return to a normal temperature regime, and the process of maturation and desiccation. The properties and structure of the starch produced were followed throughout the maturational stages. Furthermore, the key enzymes involved in the carbohydrate supply to the grain were monitored. We observed differences in starch structure with well-separated effects because of heat stress and during senescence. Heat stress produced marked effects on sucrolytic enzymes in source and sink tissues. Early cessation of plant development as an indirect consequence of the heat wave was identified as the major contributor to final yield loss from the stress, highlighting the importance for functional stay-green traits for the development of heat-resistant cereals.",

author = "Cuesta-Seijo, {Jose Antonio} and {De Porcellinis}, {Alice Jara} and Valente, {Angela H{\o}rdum} and Alexander Striebeck and Cynthia Voss and Lucia Marri and Andreas Hansson and Jansson, {Anita M} and Dinesen, {Malene Hessellund} and Fangel, {Jonatan Ulrik} and Jesper Harholt and Milan Popovic and Mercedes Thieme and Anton Hochmuth and Zeeman, {Samuel C} and Mikkelsen, {Teis N{\o}rgaard} and J{\o}rgensen, {Rikke Bagger} and Roitsch, {Thomas Georg} and M{\o}ller, {Birger Lindberg} and Ilka Braumann",

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AU - Cuesta-Seijo, Jose Antonio

AU - De Porcellinis, Alice Jara

AU - Valente, Angela Hørdum

AU - Striebeck, Alexander

AU - Voss, Cynthia

AU - Marri, Lucia

AU - Hansson, Andreas

AU - Jansson, Anita M

AU - Dinesen, Malene Hessellund

AU - Fangel, Jonatan Ulrik

AU - Harholt, Jesper

AU - Popovic, Milan

AU - Thieme, Mercedes

AU - Hochmuth, Anton

AU - Zeeman, Samuel C

AU - Mikkelsen, Teis Nørgaard

AU - Jørgensen, Rikke Bagger

AU - Roitsch, Thomas Georg

AU - Møller, Birger Lindberg

AU - Braumann, Ilka

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Abiotic environmental stresses have a negative impact on the yield and quality of crops. Understanding these stresses is an essential enabler for mitigating breeding strategies and it becomes more important as the frequency of extreme weather conditions increases due to climate change. This study analyses the response of barley (Hordeum vulgare L.) to a heat wave during grain filling in three distinct stages: the heat wave itself, the return to a normal temperature regime, and the process of maturation and desiccation. The properties and structure of the starch produced were followed throughout the maturational stages. Furthermore, the key enzymes involved in the carbohydrate supply to the grain were monitored. We observed differences in starch structure with well-separated effects because of heat stress and during senescence. Heat stress produced marked effects on sucrolytic enzymes in source and sink tissues. Early cessation of plant development as an indirect consequence of the heat wave was identified as the major contributor to final yield loss from the stress, highlighting the importance for functional stay-green traits for the development of heat-resistant cereals.

AB - Abiotic environmental stresses have a negative impact on the yield and quality of crops. Understanding these stresses is an essential enabler for mitigating breeding strategies and it becomes more important as the frequency of extreme weather conditions increases due to climate change. This study analyses the response of barley (Hordeum vulgare L.) to a heat wave during grain filling in three distinct stages: the heat wave itself, the return to a normal temperature regime, and the process of maturation and desiccation. The properties and structure of the starch produced were followed throughout the maturational stages. Furthermore, the key enzymes involved in the carbohydrate supply to the grain were monitored. We observed differences in starch structure with well-separated effects because of heat stress and during senescence. Heat stress produced marked effects on sucrolytic enzymes in source and sink tissues. Early cessation of plant development as an indirect consequence of the heat wave was identified as the major contributor to final yield loss from the stress, highlighting the importance for functional stay-green traits for the development of heat-resistant cereals.

U2 - 10.1093/pcp/pcz155

DO - 10.1093/pcp/pcz155

M3 - Journal article

C2 - 31397873

SN - 0032-0781

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

ER -

Amylopectin chain length dynamics and activity signatures of key carbon metabolic enzymes highlight early maturation as culprit for yield reduction of barley endosperm starch after heat stress

Abstract

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