Altered mitochondrial regulation in quadriceps muscles of patients with COPD

Ashley I Naimi; Jean Bourbeau; Helene Perrault; Jacinthe Baril; Cynthia Wright-Paradis; Amanda Rossi; Tanja Taivassalo; A William Sheel; Rasmus Rabøl; Flemming Dela; Robert Christopher Boushel

doi:10.1111/j.1475-097x.2010.00988.x

Altered mitochondrial regulation in quadriceps muscles of patients with COPD

Ashley I Naimi, Jean Bourbeau, Helene Perrault, Jacinthe Baril, Cynthia Wright-Paradis, Amanda Rossi, Tanja Taivassalo, A William Sheel, Rasmus Rabøl, Flemming Dela, Robert Christopher Boushel

33 Citations (Scopus)

Abstract

Evidence exists for locomotor muscle impairment in patients with chronic obstructive pulmonary disease (COPD), including fiber type alterations and reduced mitochondrial oxidative capacity. In this study high-resolution respirometry was used to quantify oxygen flux in permeabilized fibres from biopsies of the vastus lateralis muscle in patients with COPD and compared to healthy control subjects. The main findings of this study were that (i) routine state 2 respiration was higher in COPD; (ii) state 3 respiration in the presence of ADP was similar in both groups with substrate supply of electrons to complex I (COPD 38·28 ± 3·58 versus control 42·85 ± 3·10 pmol s^-1 mg tissue^-1), but O₂ flux with addition of succinate was lower in COPD patients (COPD 63·72 ± 6·33 versus control 95·73 ± 6·53 pmol s^-1 mg tissue^-1); (iii) excess capacity of cytochrome c oxidase in COPD patients was only ~50% that of control subjects. These results indicate that quadriceps muscle mitochondrial function is altered in patients with COPD. The regulatory mechanisms underlying these functional abnormalities remain to be uncovered.

Original language	English
Journal	Clinical Physiology and Functional Imaging
Volume	31
Issue number	2
Pages (from-to)	124-31
Number of pages	8
ISSN	1475-0961
DOIs	https://doi.org/10.1111/j.1475-097x.2010.00988.x
Publication status	Published - Mar 2011

Keywords

Adenosine Diphosphate
Aged
Biopsy
Case-Control Studies
Cell Respiration
Denmark
Electron Transport Chain Complex Proteins
Electron Transport Complex IV
Energy Metabolism
Humans
Lung
Male
Middle Aged
Mitochondria, Muscle
Oxygen Consumption
Pulmonary Disease, Chronic Obstructive
Quadriceps Muscle
Respiratory Function Tests
Succinic Acid

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title = "Altered mitochondrial regulation in quadriceps muscles of patients with COPD",

abstract = "Evidence exists for locomotor muscle impairment in patients with chronic obstructive pulmonary disease (COPD), including fiber type alterations and reduced mitochondrial oxidative capacity. In this study high-resolution respirometry was used to quantify oxygen flux in permeabilized fibres from biopsies of the vastus lateralis muscle in patients with COPD and compared to healthy control subjects. The main findings of this study were that (i) routine state 2 respiration was higher in COPD; (ii) state 3 respiration in the presence of ADP was similar in both groups with substrate supply of electrons to complex I (COPD 38·28 ± 3·58 versus control 42·85 ± 3·10 pmol s-1 mg tissue-1), but O2 flux with addition of succinate was lower in COPD patients (COPD 63·72 ± 6·33 versus control 95·73 ± 6·53 pmol s-1 mg tissue-1); (iii) excess capacity of cytochrome c oxidase in COPD patients was only ~50% that of control subjects. These results indicate that quadriceps muscle mitochondrial function is altered in patients with COPD. The regulatory mechanisms underlying these functional abnormalities remain to be uncovered.",

keywords = "Adenosine Diphosphate, Aged, Biopsy, Case-Control Studies, Cell Respiration, Denmark, Electron Transport Chain Complex Proteins, Electron Transport Complex IV, Energy Metabolism, Humans, Lung, Male, Middle Aged, Mitochondria, Muscle, Oxygen Consumption, Pulmonary Disease, Chronic Obstructive, Quadriceps Muscle, Respiratory Function Tests, Succinic Acid",

author = "Naimi, {Ashley I} and Jean Bourbeau and Helene Perrault and Jacinthe Baril and Cynthia Wright-Paradis and Amanda Rossi and Tanja Taivassalo and Sheel, {A William} and Rasmus Rab{\o}l and Flemming Dela and Boushel, {Robert Christopher}",

note = "{\textcopyright} 2010 The Authors. Clinical Physiology and Functional Imaging {\textcopyright} 2010 Scandinavian Society of Clinical Physiology and Nuclear Medicine.",

year = "2011",

month = mar,

doi = "10.1111/j.1475-097x.2010.00988.x",

language = "English",

volume = "31",

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journal = "Clinical Physiology and Functional Imaging",

issn = "1475-0961",

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T1 - Altered mitochondrial regulation in quadriceps muscles of patients with COPD

AU - Naimi, Ashley I

AU - Bourbeau, Jean

AU - Perrault, Helene

AU - Baril, Jacinthe

AU - Wright-Paradis, Cynthia

AU - Rossi, Amanda

AU - Taivassalo, Tanja

AU - Sheel, A William

AU - Rabøl, Rasmus

AU - Dela, Flemming

AU - Boushel, Robert Christopher

PY - 2011/3

Y1 - 2011/3

N2 - Evidence exists for locomotor muscle impairment in patients with chronic obstructive pulmonary disease (COPD), including fiber type alterations and reduced mitochondrial oxidative capacity. In this study high-resolution respirometry was used to quantify oxygen flux in permeabilized fibres from biopsies of the vastus lateralis muscle in patients with COPD and compared to healthy control subjects. The main findings of this study were that (i) routine state 2 respiration was higher in COPD; (ii) state 3 respiration in the presence of ADP was similar in both groups with substrate supply of electrons to complex I (COPD 38·28 ± 3·58 versus control 42·85 ± 3·10 pmol s-1 mg tissue-1), but O2 flux with addition of succinate was lower in COPD patients (COPD 63·72 ± 6·33 versus control 95·73 ± 6·53 pmol s-1 mg tissue-1); (iii) excess capacity of cytochrome c oxidase in COPD patients was only ~50% that of control subjects. These results indicate that quadriceps muscle mitochondrial function is altered in patients with COPD. The regulatory mechanisms underlying these functional abnormalities remain to be uncovered.

AB - Evidence exists for locomotor muscle impairment in patients with chronic obstructive pulmonary disease (COPD), including fiber type alterations and reduced mitochondrial oxidative capacity. In this study high-resolution respirometry was used to quantify oxygen flux in permeabilized fibres from biopsies of the vastus lateralis muscle in patients with COPD and compared to healthy control subjects. The main findings of this study were that (i) routine state 2 respiration was higher in COPD; (ii) state 3 respiration in the presence of ADP was similar in both groups with substrate supply of electrons to complex I (COPD 38·28 ± 3·58 versus control 42·85 ± 3·10 pmol s-1 mg tissue-1), but O2 flux with addition of succinate was lower in COPD patients (COPD 63·72 ± 6·33 versus control 95·73 ± 6·53 pmol s-1 mg tissue-1); (iii) excess capacity of cytochrome c oxidase in COPD patients was only ~50% that of control subjects. These results indicate that quadriceps muscle mitochondrial function is altered in patients with COPD. The regulatory mechanisms underlying these functional abnormalities remain to be uncovered.

KW - Adenosine Diphosphate

KW - Aged

KW - Biopsy

KW - Case-Control Studies

KW - Cell Respiration

KW - Denmark

KW - Electron Transport Chain Complex Proteins

KW - Electron Transport Complex IV

KW - Energy Metabolism

KW - Humans

KW - Lung

KW - Male

KW - Middle Aged

KW - Mitochondria, Muscle

KW - Oxygen Consumption

KW - Pulmonary Disease, Chronic Obstructive

KW - Quadriceps Muscle

KW - Respiratory Function Tests

KW - Succinic Acid

U2 - 10.1111/j.1475-097x.2010.00988.x

DO - 10.1111/j.1475-097x.2010.00988.x

M3 - Journal article

C2 - 21091605

SN - 1475-0961

VL - 31

SP - 124

EP - 131

JO - Clinical Physiology and Functional Imaging

JF - Clinical Physiology and Functional Imaging

IS - 2

ER -

Altered mitochondrial regulation in quadriceps muscles of patients with COPD

Abstract

Keywords

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