ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death

Berit Rahbek Høj; Peter Jonas Marstrand la Cour; Jens Mollerup; Martin Werner Berchtold

doi:10.1016/j.bbrc.2008.11.021

ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death

Berit Rahbek Høj, Peter Jonas Marstrand la Cour, Jens Mollerup, Martin Werner Berchtold

Cell Biology and Physiology

31 Citations (Scopus)

Abstract

ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by facilitating the passage through checkpoints in the G2/M cell cycle phase.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	378
Issue number	1
Pages (from-to)	145-8
Number of pages	3
ISSN	0006-291X
DOIs	https://doi.org/10.1016/j.bbrc.2008.11.021
Publication status	Published - 2009

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title = "ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death",

abstract = "ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by facilitating the passage through checkpoints in the G2/M cell cycle phase.",

author = "H{\o}j, {Berit Rahbek} and {la Cour}, {Peter Jonas Marstrand} and Jens Mollerup and Berchtold, {Martin Werner}",

note = "KEYWORDS: Calcium-binding proteins; Apoptosis-linked gene-2 (ALG-2); siRNA; Apoptosis; Cell cycle; Caspase",

year = "2009",

doi = "10.1016/j.bbrc.2008.11.021",

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T1 - ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death

AU - Høj, Berit Rahbek

AU - la Cour, Peter Jonas Marstrand

AU - Mollerup, Jens

AU - Berchtold, Martin Werner

N1 - KEYWORDS: Calcium-binding proteins; Apoptosis-linked gene-2 (ALG-2); siRNA; Apoptosis; Cell cycle; Caspase

PY - 2009

Y1 - 2009

N2 - ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by facilitating the passage through checkpoints in the G2/M cell cycle phase.

AB - ALG-2 (apoptosis-linked gene-2 encoded protein) has been shown to be upregulated in a variety of human tumors questioning its previously assumed pro-apoptotic function. The aim of the present study was to obtain insights into the role of ALG-2 in human cancer cells. We show that ALG-2 downregulation induces accumulation of HeLa cells in the G2/M cell cycle phase and increases the amount of early apoptotic and dead cells. Caspase inhibition by the pan-caspase inhibitor zVAD-fmk attenuated the increase in the amount of dead cells following ALG-2 downregulation. Thus, our results indicate that ALG-2 has an anti-apoptotic function in HeLa cells by facilitating the passage through checkpoints in the G2/M cell cycle phase.

U2 - 10.1016/j.bbrc.2008.11.021

DO - 10.1016/j.bbrc.2008.11.021

M3 - Journal article

C2 - 19013425

SN - 0006-291X

VL - 378

SP - 145

EP - 148

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

ALG-2 knockdown in HeLa cells results in G2/M cell cycle phase accumulation and cell death

Abstract

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