TY - JOUR
T1 - Age-related and depot-specific changes in white adipose tissue of growth hormone receptor-null mice
AU - Sackmann-Sala, Lucila
AU - Berryman, Darlene E.
AU - Lubbers, Ellen R.
AU - Zhang, Han
AU - Vesel, Clare B.
AU - Troike, Katie M.
AU - Gosney, Elahu S.
AU - List, Edward O.
AU - Kopchick, John J.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Growth hormone receptor-null (GHR-/-) mice are dwarf, insulin sensitive, and long-lived in spite of increased adiposity. However, their adiposity is not uniform, with select white adipose tissue (WAT) depots enlarged. To study WAT depot-specific effects on insulin sensitivity and life span, we analyzed individual WAT depots of 12- and 24-month-old GHR -/- and wild-type (WT) mice, as well as their plasma levels of selected hormones. Adipocyte sizes and plasma insulin, leptin, and adiponectin levels decreased with age in both GHR-/- and WT mice. Two-dimensional gel electrophoresis proteomes of WAT depots were similar among groups, but several proteins involved in endocytosis and/or cytoskeletal organization (Ehd2, S100A10, actin), anticoagulation (S100A10, annexin A5), and age-related conditions (alpha2-macroglobulin, apolipoprotein A-I, transthyretin) showed significant differences between genotypes. Because Ehd2 may regulate endocytosis of Glut4, we measured Glut4 levels in the WAT depots of GHR-/- and WT mice. Inguinal WAT of 12-month-old GHR-/- mice displayed lower levels of Glut4 than WT. Overall, the protein changes detected in this study offer new insights into possible mechanisms contributing to enhanced insulin sensitivity and extended life span in GHR-/- mice.
AB - Growth hormone receptor-null (GHR-/-) mice are dwarf, insulin sensitive, and long-lived in spite of increased adiposity. However, their adiposity is not uniform, with select white adipose tissue (WAT) depots enlarged. To study WAT depot-specific effects on insulin sensitivity and life span, we analyzed individual WAT depots of 12- and 24-month-old GHR -/- and wild-type (WT) mice, as well as their plasma levels of selected hormones. Adipocyte sizes and plasma insulin, leptin, and adiponectin levels decreased with age in both GHR-/- and WT mice. Two-dimensional gel electrophoresis proteomes of WAT depots were similar among groups, but several proteins involved in endocytosis and/or cytoskeletal organization (Ehd2, S100A10, actin), anticoagulation (S100A10, annexin A5), and age-related conditions (alpha2-macroglobulin, apolipoprotein A-I, transthyretin) showed significant differences between genotypes. Because Ehd2 may regulate endocytosis of Glut4, we measured Glut4 levels in the WAT depots of GHR-/- and WT mice. Inguinal WAT of 12-month-old GHR-/- mice displayed lower levels of Glut4 than WT. Overall, the protein changes detected in this study offer new insights into possible mechanisms contributing to enhanced insulin sensitivity and extended life span in GHR-/- mice.
KW - Adipose tissue depots
KW - Aging
KW - Endocytosis
KW - Glut4
KW - Growth hormone receptor
UR - http://www.scopus.com/inward/record.url?scp=84890545592&partnerID=8YFLogxK
U2 - 10.1093/gerona/glt110
DO - 10.1093/gerona/glt110
M3 - Journal article
C2 - 23873966
AN - SCOPUS:84890545592
SN - 1079-5006
VL - 69
SP - 34
EP - 43
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
IS - 1
ER -