Advances and perspectives in tissue clearing using CLARITY

TY - JOUR

T1 - Advances and perspectives in tissue clearing using CLARITY

AU - Reveles Jensen, Kristian

AU - Berg, Rune W.

PY - 2017/12

Y1 - 2017/12

N2 - CLARITY is a tissue clearing method, which enables immunostaining and imaging of large volumes for 3D-reconstruction. The method was initially time-consuming, expensive and relied on electrophoresis to remove lipids to make the tissue transparent. Since then several improvements and simplifications have emerged, such as passive clearing (PACT) and methods to improve tissue staining. Here, we review advances and compare current applications with the aim of highlighting needed improvements as well as aiding selection of the specific protocol for use in future investigations.

AB - CLARITY is a tissue clearing method, which enables immunostaining and imaging of large volumes for 3D-reconstruction. The method was initially time-consuming, expensive and relied on electrophoresis to remove lipids to make the tissue transparent. Since then several improvements and simplifications have emerged, such as passive clearing (PACT) and methods to improve tissue staining. Here, we review advances and compare current applications with the aim of highlighting needed improvements as well as aiding selection of the specific protocol for use in future investigations.

U2 - 10.1016/j.jchemneu.2017.07.005

DO - 10.1016/j.jchemneu.2017.07.005

M3 - Review

C2 - 28728966

SN - 0891-0618

VL - 86

SP - 19

EP - 34

JO - Journal of Chemical Neuroanatomy

JF - Journal of Chemical Neuroanatomy

ER -