Advanced microtechnologies for detection of chromosome abnormalities by fluorescent in situ hybridization

Dorota Kwasny; Indumathi Vedarethinam; Pranjul Jaykumar Shah; Maria Dimaki; Asli Silahtaroglu; Zeynep Tumer; Winnie Edith Svendsen

doi:10.1007/s10544-011-9622-7

Advanced microtechnologies for detection of chromosome abnormalities by fluorescent in situ hybridization

Dorota Kwasny, Indumathi Vedarethinam, Pranjul Jaykumar Shah, Maria Dimaki, Asli Silahtaroglu, Zeynep Tumer, Winnie Edith Svendsen

9 Citations (Scopus)

Abstract

Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time. The recent focus on the development of automated systems for performing FISH on chip is summarized in this review.

Original language	English
Journal	Biomedical Microdevices
Volume	14
Issue number	3
Pages (from-to)	453-60
Number of pages	8
ISSN	1387-2176
DOIs	https://doi.org/10.1007/s10544-011-9622-7
Publication status	Published - Jun 2012

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title = "Advanced microtechnologies for detection of chromosome abnormalities by fluorescent in situ hybridization",

abstract = "Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time. The recent focus on the development of automated systems for performing FISH on chip is summarized in this review.",

author = "Dorota Kwasny and Indumathi Vedarethinam and Shah, {Pranjul Jaykumar} and Maria Dimaki and Asli Silahtaroglu and Zeynep Tumer and Svendsen, {Winnie Edith}",

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T1 - Advanced microtechnologies for detection of chromosome abnormalities by fluorescent in situ hybridization

AU - Kwasny, Dorota

AU - Vedarethinam, Indumathi

AU - Shah, Pranjul Jaykumar

AU - Dimaki, Maria

AU - Silahtaroglu, Asli

AU - Tumer, Zeynep

AU - Svendsen, Winnie Edith

PY - 2012/6

Y1 - 2012/6

N2 - Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time. The recent focus on the development of automated systems for performing FISH on chip is summarized in this review.

AB - Cytogenetic and molecular cytogenetic analyses, which aim to detect chromosome abnormalities, are routinely performed in cytogenetic laboratories all over the world. Traditional cytogenetic studies are performed by analyzing the banding pattern of chromosomes, and are complemented by molecular cytogenetic techniques such as fluorescent in situ hybridization (FISH). To improve FISH application in cytogenetic analysis the issues with long experimental time, high volumes of expensive reagents and requirement for trained technicians need to be addressed. The protocol has recently evolved towards on chip detection of chromosome abnormalities with the development of microsystems for FISH analysis. The challenges addressed by the developed microsystems are mainly the automation of the assay performance, reduction in probe volume, as well as reduction of assay time. The recent focus on the development of automated systems for performing FISH on chip is summarized in this review.

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DO - 10.1007/s10544-011-9622-7

M3 - Journal article

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SN - 1387-2176

VL - 14

SP - 453

EP - 460

JO - Biomedical Microdevices

JF - Biomedical Microdevices

IS - 3

ER -

Advanced microtechnologies for detection of chromosome abnormalities by fluorescent in situ hybridization

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