Acute and perinatal programming effects of a fat-rich diet on rat muscle mitochondrial function and hepatic lipid accumulation

TY - JOUR

T1 - Acute and perinatal programming effects of a fat-rich diet on rat muscle mitochondrial function and hepatic lipid accumulation

AU - Hellgren, Lars I

AU - Jensen, Runa I

AU - Waterstradt, Michelle S Gundel

AU - Quistorff, Bjørn

AU - Lauritzen, Lotte

N1 - CURIS 2014 NEXS 213

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Conclusion Intake of a fat-rich diet during pregnancy and lactation reduced the age-induced increases in un-coupled fat oxidation.Results At 20 days, maternal high-fat diet caused increased Vo2max with pyruvate as substrate (p = 0.047), at 70 days, pups born by C-dams, but not those born by high-fat-dams, showed increased oxidation of palmitoylcarnitine in the absence of ADP (p = 0.018). Rates of ADP-stimulated oxygen consumption, maximal respiratory capacity and mitochondrial respiratory control ratio with pyruvate, increased post weaning (p < 0.001), whereas respiratory control ratio with palmitoylcarnitine decreased (p = 0.013). The increase in respiratory control ratio was most pronounced in pups from C-dams (p = 0.05). The high-fat-diet caused pronounced hepatic steatosis in pups at weaning (p < 0.001), without concomitant ceramide accumulation, while high-fat-feeding after weaning induced triacylglycerol and ceramide accumulation (p < 0.01), regardless of maternal diet.Design Sprague-Dawley rats were fed a high-fat (20% w/w) or a control diet (chow, C) from 10 days before pregnancy and throughout lactation. At weaning the litters were split into two groups; one was continued on the maternal diet and the other was fed low-fat chow. Sample Skeletal muscle mitochondria and liver lipids.Methods Mitochondrial respiration and hepatic lipid content were determined during and after weaning, on days 20 and 70 postpartum. Main outcome measures Mitochondrial function and hepatic lipids.Objective Maternal high-fat intake during pregnancy may have long-term consequences in the offspring. Since this might relate to the capacity of mitochondrial metabolic adaptation and hepatic lipid metabolism, we investigated how maternal high-fat intake affected mitochondrial function and hepatic steatosis in the offspring.

AB - Conclusion Intake of a fat-rich diet during pregnancy and lactation reduced the age-induced increases in un-coupled fat oxidation.Results At 20 days, maternal high-fat diet caused increased Vo2max with pyruvate as substrate (p = 0.047), at 70 days, pups born by C-dams, but not those born by high-fat-dams, showed increased oxidation of palmitoylcarnitine in the absence of ADP (p = 0.018). Rates of ADP-stimulated oxygen consumption, maximal respiratory capacity and mitochondrial respiratory control ratio with pyruvate, increased post weaning (p < 0.001), whereas respiratory control ratio with palmitoylcarnitine decreased (p = 0.013). The increase in respiratory control ratio was most pronounced in pups from C-dams (p = 0.05). The high-fat-diet caused pronounced hepatic steatosis in pups at weaning (p < 0.001), without concomitant ceramide accumulation, while high-fat-feeding after weaning induced triacylglycerol and ceramide accumulation (p < 0.01), regardless of maternal diet.Design Sprague-Dawley rats were fed a high-fat (20% w/w) or a control diet (chow, C) from 10 days before pregnancy and throughout lactation. At weaning the litters were split into two groups; one was continued on the maternal diet and the other was fed low-fat chow. Sample Skeletal muscle mitochondria and liver lipids.Methods Mitochondrial respiration and hepatic lipid content were determined during and after weaning, on days 20 and 70 postpartum. Main outcome measures Mitochondrial function and hepatic lipids.Objective Maternal high-fat intake during pregnancy may have long-term consequences in the offspring. Since this might relate to the capacity of mitochondrial metabolic adaptation and hepatic lipid metabolism, we investigated how maternal high-fat intake affected mitochondrial function and hepatic steatosis in the offspring.

U2 - 10.1111/aogs.12458

DO - 10.1111/aogs.12458

M3 - Journal article

C2 - 25052904

SN - 0001-6349

VL - 93

SP - 1170

EP - 1180

JO - Acta Obstetricia et Gynecologica Scandinavica

JF - Acta Obstetricia et Gynecologica Scandinavica

IS - 11

ER -