Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis

Charlotte Bremer Christensen; Jens Soelberg; Christen R Stensvold; Anna Jäger

doi:10.1016/j.jep.2015.03.006

Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis

Charlotte Bremer Christensen, Jens Soelberg, Christen R Stensvold, Anna Jäger

13 Citations (Scopus)

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE: The plants tested in this study were examples of plants historically used to treat or alleviate several types of stomach disorders manifested by e.g. stomachache, diarrhoea or dysentery. These plants have been consumed typically as a decoction, sometimes mixed with other flavourings. The aim of this study was to evaluate the anti-Blastocystis activity of 24 plant parts from 21 medicinal plants from Ghana.

MATERIALS AND METHODS: The medicinal plants were collected in the Greater Accra region of Ghana. Every plant part was tested in three different extracts; an ethanolic, a warm, and a cold water extract, at a final concentration of 1mg/mL for the initial screening, and in a range from 0.0156 to 1mg/mL for determination of inhibitory concentrations. The obligate anaerobic parasitic gut protist Blastocystis (subtype 4) was used as a 48h old subcultivated isolate in the final concentration of 10(6) cells/mL. Plant extracts inoculated with Blastocystis were incubated at 37°C for 24h and 48h. Both MIC minimum inhibitory concentration (MIC90) assays and minimal lethal concentration (MLC) assays were performed after 24h and 48h. The half maximal inhibitory concentration (IC50) was derived after 24h and 48h. Antimicrobial activity was tested against two Gram-positive and two Gram-negative bacteria for all 24 plant parts at a final concentration of 1mg/mL.

RESULTS: Screening of the 24 different plant parts showed significant anti-Blastocystis activity of six of the ethanolic extracts: Mallotus oppositifolius, IC50, 24h 27.8µg/mL; Vemonia colorata, IC50, 24h 117.9µg/mL; Zanthoxylum zanthoxyloides, cortex IC50, 24h 255.6µg/mL; Clausena anisata, IC50, 24h 314.0µg/mL; Z. zanthoxyloides, radix IC50, 24h 335.7µg/mL and Eythrina senegalensis, IC50, 24h 527.6µg/mL. The reference anti-protozoal agent metronidazole (MTZ) had an IC50, 24h of 7.6µg/mL. Only C. anisata showed antimicrobial activity at a concentration of 800µg/mL.

CONCLUSION: Six ethanolic plant extracts showed significant anti-parasitic activity against Blastocystis. M. oppositifolius showed nearly as good activity as the reference anti-protozoal drug MTZ. Historically, the active plants found in this study have been used against dysentery, diarrhoea or other stomach disorders. Nowadays they are not used specifically for dysentery, but they are being used as medicinal plants against various stomach disorders.

Original language	English
Journal	Journal of Ethnopharmacology
Volume	174
Issue number	4
Pages (from-to)	569–575
Number of pages	7
ISSN	0378-8741
DOIs	https://doi.org/10.1016/j.jep.2015.03.006
Publication status	Published - 4 Nov 2015

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@article{a2b5716b544748229e5ca9395c3b4b59,

title = "Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis",

abstract = "ETHNOPHARMACOLOGICAL RELEVANCE: The plants tested in this study were examples of plants historically used to treat or alleviate several types of stomach disorders manifested by e.g. stomachache, diarrhoea or dysentery. These plants have been consumed typically as a decoction, sometimes mixed with other flavourings. The aim of this study was to evaluate the anti-Blastocystis activity of 24 plant parts from 21 medicinal plants from Ghana.MATERIALS AND METHODS: The medicinal plants were collected in the Greater Accra region of Ghana. Every plant part was tested in three different extracts; an ethanolic, a warm, and a cold water extract, at a final concentration of 1mg/mL for the initial screening, and in a range from 0.0156 to 1mg/mL for determination of inhibitory concentrations. The obligate anaerobic parasitic gut protist Blastocystis (subtype 4) was used as a 48h old subcultivated isolate in the final concentration of 10(6) cells/mL. Plant extracts inoculated with Blastocystis were incubated at 37°C for 24h and 48h. Both MIC minimum inhibitory concentration (MIC90) assays and minimal lethal concentration (MLC) assays were performed after 24h and 48h. The half maximal inhibitory concentration (IC50) was derived after 24h and 48h. Antimicrobial activity was tested against two Gram-positive and two Gram-negative bacteria for all 24 plant parts at a final concentration of 1mg/mL.RESULTS: Screening of the 24 different plant parts showed significant anti-Blastocystis activity of six of the ethanolic extracts: Mallotus oppositifolius, IC50, 24h 27.8µg/mL; Vemonia colorata, IC50, 24h 117.9µg/mL; Zanthoxylum zanthoxyloides, cortex IC50, 24h 255.6µg/mL; Clausena anisata, IC50, 24h 314.0µg/mL; Z. zanthoxyloides, radix IC50, 24h 335.7µg/mL and Eythrina senegalensis, IC50, 24h 527.6µg/mL. The reference anti-protozoal agent metronidazole (MTZ) had an IC50, 24h of 7.6µg/mL. Only C. anisata showed antimicrobial activity at a concentration of 800µg/mL.CONCLUSION: Six ethanolic plant extracts showed significant anti-parasitic activity against Blastocystis. M. oppositifolius showed nearly as good activity as the reference anti-protozoal drug MTZ. Historically, the active plants found in this study have been used against dysentery, diarrhoea or other stomach disorders. Nowadays they are not used specifically for dysentery, but they are being used as medicinal plants against various stomach disorders.",

author = "{Bremer Christensen}, Charlotte and Jens Soelberg and Stensvold, {Christen R} and Anna J{\"a}ger",

year = "2015",

month = nov,

day = "4",

doi = "10.1016/j.jep.2015.03.006",

language = "English",

volume = "174",

pages = "569–575",

journal = "Journal of Ethnopharmacology",

issn = "0378-8741",

publisher = "Elsevier Ireland Ltd",

number = "4",

}

TY - JOUR

T1 - Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis

AU - Bremer Christensen, Charlotte

AU - Soelberg, Jens

AU - Stensvold, Christen R

AU - Jäger, Anna

PY - 2015/11/4

Y1 - 2015/11/4

N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The plants tested in this study were examples of plants historically used to treat or alleviate several types of stomach disorders manifested by e.g. stomachache, diarrhoea or dysentery. These plants have been consumed typically as a decoction, sometimes mixed with other flavourings. The aim of this study was to evaluate the anti-Blastocystis activity of 24 plant parts from 21 medicinal plants from Ghana.MATERIALS AND METHODS: The medicinal plants were collected in the Greater Accra region of Ghana. Every plant part was tested in three different extracts; an ethanolic, a warm, and a cold water extract, at a final concentration of 1mg/mL for the initial screening, and in a range from 0.0156 to 1mg/mL for determination of inhibitory concentrations. The obligate anaerobic parasitic gut protist Blastocystis (subtype 4) was used as a 48h old subcultivated isolate in the final concentration of 10(6) cells/mL. Plant extracts inoculated with Blastocystis were incubated at 37°C for 24h and 48h. Both MIC minimum inhibitory concentration (MIC90) assays and minimal lethal concentration (MLC) assays were performed after 24h and 48h. The half maximal inhibitory concentration (IC50) was derived after 24h and 48h. Antimicrobial activity was tested against two Gram-positive and two Gram-negative bacteria for all 24 plant parts at a final concentration of 1mg/mL.RESULTS: Screening of the 24 different plant parts showed significant anti-Blastocystis activity of six of the ethanolic extracts: Mallotus oppositifolius, IC50, 24h 27.8µg/mL; Vemonia colorata, IC50, 24h 117.9µg/mL; Zanthoxylum zanthoxyloides, cortex IC50, 24h 255.6µg/mL; Clausena anisata, IC50, 24h 314.0µg/mL; Z. zanthoxyloides, radix IC50, 24h 335.7µg/mL and Eythrina senegalensis, IC50, 24h 527.6µg/mL. The reference anti-protozoal agent metronidazole (MTZ) had an IC50, 24h of 7.6µg/mL. Only C. anisata showed antimicrobial activity at a concentration of 800µg/mL.CONCLUSION: Six ethanolic plant extracts showed significant anti-parasitic activity against Blastocystis. M. oppositifolius showed nearly as good activity as the reference anti-protozoal drug MTZ. Historically, the active plants found in this study have been used against dysentery, diarrhoea or other stomach disorders. Nowadays they are not used specifically for dysentery, but they are being used as medicinal plants against various stomach disorders.

AB - ETHNOPHARMACOLOGICAL RELEVANCE: The plants tested in this study were examples of plants historically used to treat or alleviate several types of stomach disorders manifested by e.g. stomachache, diarrhoea or dysentery. These plants have been consumed typically as a decoction, sometimes mixed with other flavourings. The aim of this study was to evaluate the anti-Blastocystis activity of 24 plant parts from 21 medicinal plants from Ghana.MATERIALS AND METHODS: The medicinal plants were collected in the Greater Accra region of Ghana. Every plant part was tested in three different extracts; an ethanolic, a warm, and a cold water extract, at a final concentration of 1mg/mL for the initial screening, and in a range from 0.0156 to 1mg/mL for determination of inhibitory concentrations. The obligate anaerobic parasitic gut protist Blastocystis (subtype 4) was used as a 48h old subcultivated isolate in the final concentration of 10(6) cells/mL. Plant extracts inoculated with Blastocystis were incubated at 37°C for 24h and 48h. Both MIC minimum inhibitory concentration (MIC90) assays and minimal lethal concentration (MLC) assays were performed after 24h and 48h. The half maximal inhibitory concentration (IC50) was derived after 24h and 48h. Antimicrobial activity was tested against two Gram-positive and two Gram-negative bacteria for all 24 plant parts at a final concentration of 1mg/mL.RESULTS: Screening of the 24 different plant parts showed significant anti-Blastocystis activity of six of the ethanolic extracts: Mallotus oppositifolius, IC50, 24h 27.8µg/mL; Vemonia colorata, IC50, 24h 117.9µg/mL; Zanthoxylum zanthoxyloides, cortex IC50, 24h 255.6µg/mL; Clausena anisata, IC50, 24h 314.0µg/mL; Z. zanthoxyloides, radix IC50, 24h 335.7µg/mL and Eythrina senegalensis, IC50, 24h 527.6µg/mL. The reference anti-protozoal agent metronidazole (MTZ) had an IC50, 24h of 7.6µg/mL. Only C. anisata showed antimicrobial activity at a concentration of 800µg/mL.CONCLUSION: Six ethanolic plant extracts showed significant anti-parasitic activity against Blastocystis. M. oppositifolius showed nearly as good activity as the reference anti-protozoal drug MTZ. Historically, the active plants found in this study have been used against dysentery, diarrhoea or other stomach disorders. Nowadays they are not used specifically for dysentery, but they are being used as medicinal plants against various stomach disorders.

U2 - 10.1016/j.jep.2015.03.006

DO - 10.1016/j.jep.2015.03.006

M3 - Journal article

C2 - 25773490

SN - 0378-8741

VL - 174

SP - 569

EP - 575

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

IS - 4

ER -

Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis

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