Activity of L-alpha-amino acids at the promiscuous goldfish odorant receptor 5.24

Bolette Christiansen; Petrine Wellendorph; Hans Bräuner-Osborne

doi:10.1016/j.ejphar.2006.02.015

Activity of L-alpha-amino acids at the promiscuous goldfish odorant receptor 5.24

Bolette Christiansen, Petrine Wellendorph, Hans Bräuner-Osborne

9 Citations (Scopus)

Abstract

The goldfish odorant receptor 5.24 is a member of family C of G protein-coupled receptors and is closely related to the human receptor GPRC6A. Receptor 5.24 has previously been shown to have binding affinity for L-alpha-amino acids, especially the basic amino acids arginine and lysine. Here we report the agonist activities of the 20 proteinogenic L-alpha-amino acids, and L-ornithine and L-citrulline, measured in an intracellular calcium release assay in mammalian tsA cells. The results show that receptor 5.24 is broadly activated by 19 of the tested L-alpha-amino acids and displays a preference for basic amino acids.

Original language	English
Journal	European Journal of Pharmacology
Volume	536
Issue number	1-2
Pages (from-to)	98-101
ISSN	0014-2999
DOIs	https://doi.org/10.1016/j.ejphar.2006.02.015
Publication status	Published - 24 Apr 2006

Keywords

Amino Acids
Aniline Compounds
Animals
Binding, Competitive
Calcium
Cell Line
Dose-Response Relationship, Drug
Fluorescent Dyes
Gene Expression
Goldfish
Humans
Intracellular Fluid
Receptors, Odorant
Transfection
Xanthenes

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10.1016/j.ejphar.2006.02.015

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title = "Activity of L-alpha-amino acids at the promiscuous goldfish odorant receptor 5.24",

abstract = "The goldfish odorant receptor 5.24 is a member of family C of G protein-coupled receptors and is closely related to the human receptor GPRC6A. Receptor 5.24 has previously been shown to have binding affinity for L-alpha-amino acids, especially the basic amino acids arginine and lysine. Here we report the agonist activities of the 20 proteinogenic L-alpha-amino acids, and L-ornithine and L-citrulline, measured in an intracellular calcium release assay in mammalian tsA cells. The results show that receptor 5.24 is broadly activated by 19 of the tested L-alpha-amino acids and displays a preference for basic amino acids.",

keywords = "Amino Acids, Aniline Compounds, Animals, Binding, Competitive, Calcium, Cell Line, Dose-Response Relationship, Drug, Fluorescent Dyes, Gene Expression, Goldfish, Humans, Intracellular Fluid, Receptors, Odorant, Transfection, Xanthenes",

author = "Bolette Christiansen and Petrine Wellendorph and Hans Br{\"a}uner-Osborne",

year = "2006",

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day = "24",

doi = "10.1016/j.ejphar.2006.02.015",

language = "English",

volume = "536",

pages = "98--101",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Activity of L-alpha-amino acids at the promiscuous goldfish odorant receptor 5.24

AU - Christiansen, Bolette

AU - Wellendorph, Petrine

AU - Bräuner-Osborne, Hans

PY - 2006/4/24

Y1 - 2006/4/24

N2 - The goldfish odorant receptor 5.24 is a member of family C of G protein-coupled receptors and is closely related to the human receptor GPRC6A. Receptor 5.24 has previously been shown to have binding affinity for L-alpha-amino acids, especially the basic amino acids arginine and lysine. Here we report the agonist activities of the 20 proteinogenic L-alpha-amino acids, and L-ornithine and L-citrulline, measured in an intracellular calcium release assay in mammalian tsA cells. The results show that receptor 5.24 is broadly activated by 19 of the tested L-alpha-amino acids and displays a preference for basic amino acids.

AB - The goldfish odorant receptor 5.24 is a member of family C of G protein-coupled receptors and is closely related to the human receptor GPRC6A. Receptor 5.24 has previously been shown to have binding affinity for L-alpha-amino acids, especially the basic amino acids arginine and lysine. Here we report the agonist activities of the 20 proteinogenic L-alpha-amino acids, and L-ornithine and L-citrulline, measured in an intracellular calcium release assay in mammalian tsA cells. The results show that receptor 5.24 is broadly activated by 19 of the tested L-alpha-amino acids and displays a preference for basic amino acids.

KW - Amino Acids

KW - Aniline Compounds

KW - Animals

KW - Binding, Competitive

KW - Calcium

KW - Cell Line

KW - Dose-Response Relationship, Drug

KW - Fluorescent Dyes

KW - Gene Expression

KW - Goldfish

KW - Humans

KW - Intracellular Fluid

KW - Receptors, Odorant

KW - Transfection

KW - Xanthenes

U2 - 10.1016/j.ejphar.2006.02.015

DO - 10.1016/j.ejphar.2006.02.015

M3 - Journal article

C2 - 16546162

SN - 0014-2999

VL - 536

SP - 98

EP - 101

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 1-2

ER -

Activity of L-alpha-amino acids at the promiscuous goldfish odorant receptor 5.24

Abstract

Keywords

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