Abstract
BACKGROUND/AIM: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.
MATERIALS AND METHODS: Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 10(3) S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH2 and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.
RESULTS/CONCLUSION: Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica(+)) but not ica(-) S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica(+) or ica(-) S. aureus, suggesting a lack of specificity in this antiserum.
| Original language | English |
|---|---|
| Journal | In Vivo |
| Volume | 31 |
| Issue number | 1 |
| Pages (from-to) | 45-50 |
| Number of pages | 6 |
| ISSN | 0258-851X |
| DOIs | |
| Publication status | Published - 2017 |