Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli

T Kruse; B Blagoev; A Lobner-Olesen; M Wachi; K Sasaki; N Iwai; M Mann; K Gerdes

doi:10.1101/gad.366606

Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli

T Kruse, B Blagoev, A Lobner-Olesen, M Wachi, K Sasaki, N Iwai, M Mann, K Gerdes

Functional Genomics

110 Citations (Scopus)

Abstract

The actin-like MreB cytoskeletal protein and RNA polymerase (RNAP) have both been suggested to provide the force for chromosome segregation. Here, we identify MreB and RNAP as in vivo interaction partners. The interaction was confirmed using in vitro purified components. We also present convincing evidence that MreB and RNAP are both required for chromosome segregation in Escherichia coli. MreB is required for origin and bulk DNA segregation, whereas RNAP is required for bulk DNA, terminus, and possibly also for origin segregation. Furthermore, flow cytometric analyses show that MreB depletion and inactivation of RNAP confer virtually identical and highly unusual chromosome segregation defects. Thus, our results raise the possibility that the MreB-RNAP interaction is functionally important for chromosome segregation.

Original language	English
Journal	Genes & Development
Volume	20
Issue number	1
Pages (from-to)	113-124
Number of pages	12
ISSN	0890-9369
DOIs	https://doi.org/10.1101/gad.366606
Publication status	Published - 1 Jan 2006

Keywords

MreB
actin
RNA polymerase (RNAP)
chromosome segregation
oriC
terC

Access to Document

10.1101/gad.366606

Cite this

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title = "Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli",

abstract = "The actin-like MreB cytoskeletal protein and RNA polymerase (RNAP) have both been suggested to provide the force for chromosome segregation. Here, we identify MreB and RNAP as in vivo interaction partners. The interaction was confirmed using in vitro purified components. We also present convincing evidence that MreB and RNAP are both required for chromosome segregation in Escherichia coli. MreB is required for origin and bulk DNA segregation, whereas RNAP is required for bulk DNA, terminus, and possibly also for origin segregation. Furthermore, flow cytometric analyses show that MreB depletion and inactivation of RNAP confer virtually identical and highly unusual chromosome segregation defects. Thus, our results raise the possibility that the MreB-RNAP interaction is functionally important for chromosome segregation.",

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T1 - Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli

AU - Kruse, T

AU - Blagoev, B

AU - Lobner-Olesen, A

AU - Wachi, M

AU - Sasaki, K

AU - Iwai, N

AU - Mann, M

AU - Gerdes, K

PY - 2006/1/1

Y1 - 2006/1/1

N2 - The actin-like MreB cytoskeletal protein and RNA polymerase (RNAP) have both been suggested to provide the force for chromosome segregation. Here, we identify MreB and RNAP as in vivo interaction partners. The interaction was confirmed using in vitro purified components. We also present convincing evidence that MreB and RNAP are both required for chromosome segregation in Escherichia coli. MreB is required for origin and bulk DNA segregation, whereas RNAP is required for bulk DNA, terminus, and possibly also for origin segregation. Furthermore, flow cytometric analyses show that MreB depletion and inactivation of RNAP confer virtually identical and highly unusual chromosome segregation defects. Thus, our results raise the possibility that the MreB-RNAP interaction is functionally important for chromosome segregation.

AB - The actin-like MreB cytoskeletal protein and RNA polymerase (RNAP) have both been suggested to provide the force for chromosome segregation. Here, we identify MreB and RNAP as in vivo interaction partners. The interaction was confirmed using in vitro purified components. We also present convincing evidence that MreB and RNAP are both required for chromosome segregation in Escherichia coli. MreB is required for origin and bulk DNA segregation, whereas RNAP is required for bulk DNA, terminus, and possibly also for origin segregation. Furthermore, flow cytometric analyses show that MreB depletion and inactivation of RNAP confer virtually identical and highly unusual chromosome segregation defects. Thus, our results raise the possibility that the MreB-RNAP interaction is functionally important for chromosome segregation.

KW - MreB

KW - actin

KW - RNA polymerase (RNAP)

KW - chromosome segregation

KW - oriC

KW - terC

U2 - 10.1101/gad.366606

DO - 10.1101/gad.366606

M3 - Journal article

C2 - 16391237

SN - 0890-9369

VL - 20

SP - 113

EP - 124

JO - Genes & Development

JF - Genes & Development

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