Acquisition of resistance after continuous infection with Ascaridia galli in chickens

Tania Ferdushy; T. W. Schou; Liselotte Rothmann Norup; Tina Sørensen Dalgaard; Stig Milan Thamsborg; Peter Nejsum; A. Permin; Helle Risdahl Juul-Madsen; Niels Christian Kyvsgaard

doi:10.1017/s0031182014000742

Acquisition of resistance after continuous infection with Ascaridia galli in chickens

Tania Ferdushy, T. W. Schou, Liselotte Rothmann Norup, Tina Sørensen Dalgaard, Stig Milan Thamsborg, Peter Nejsum, A. Permin, Helle Risdahl Juul-Madsen, Niels Christian Kyvsgaard

6 Citations (Scopus)

Abstract

Acquired resistance against Ascaridia galli infection was studied in seventy-two 18-week-old white Leghorn chickens allocated to six groups (G1-G6). In order to understand the population dynamics following trickle-infection (100 eggs per chicken twice weekly), chickens of subgroups of G1 were necropsied 3 days after 1, 6 or 12 inoculations (G1A, G1B and G1C respectively), while G2-G4 were inoculated for 6 weeks. G2 was necropsied 4 weeks after the last inoculation. The number of established larvae increased initially (between G1A and G1B) but decreased after repeated inoculations (G1C, G2). G3, G4 and G5 were used to measure the efficacy of anthelminthic treatment and to monitor the acquisition of resistance following a challenge infection. At week 7 G3, G4 and G5 were treated with flubendazole for 7 days in the feed. Two weeks after treatment the chickens in G4 and G5 were challenged with 500 eggs. G6 was left as uninfected control. Necropsy at week 10 after first inoculation revealed a lower establishment rate, an impaired development and a more posterior localization of the larvae in G4 (trickle-infected-treated-challenged) compared with G5 (treated-challenged). IgY level in serum reached noticeable level at 14 dpi in G2 and G4 chickens, and in G4 chickens IgY level further increased after challenge infection. The study provides evidence that acquired resistance against A. galli in chickens leads to a significant yet incomplete protection against re-infection.

Original language	English
Journal	Parasitology
Volume	141
Issue number	12
Pages (from-to)	1603-1610
Number of pages	8
ISSN	0031-1820
DOIs	https://doi.org/10.1017/s0031182014000742
Publication status	Published - Oct 2014

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@article{09d22c2269b442f7838180e695fd4e0f,

title = "Acquisition of resistance after continuous infection with Ascaridia galli in chickens",

abstract = "Acquired resistance against Ascaridia galli infection was studied in seventy-two 18-week-old white Leghorn chickens allocated to six groups (G1-G6). In order to understand the population dynamics following trickle-infection (100 eggs per chicken twice weekly), chickens of subgroups of G1 were necropsied 3 days after 1, 6 or 12 inoculations (G1A, G1B and G1C respectively), while G2-G4 were inoculated for 6 weeks. G2 was necropsied 4 weeks after the last inoculation. The number of established larvae increased initially (between G1A and G1B) but decreased after repeated inoculations (G1C, G2). G3, G4 and G5 were used to measure the efficacy of anthelminthic treatment and to monitor the acquisition of resistance following a challenge infection. At week 7 G3, G4 and G5 were treated with flubendazole for 7 days in the feed. Two weeks after treatment the chickens in G4 and G5 were challenged with 500 eggs. G6 was left as uninfected control. Necropsy at week 10 after first inoculation revealed a lower establishment rate, an impaired development and a more posterior localization of the larvae in G4 (trickle-infected-treated-challenged) compared with G5 (treated-challenged). IgY level in serum reached noticeable level at 14 dpi in G2 and G4 chickens, and in G4 chickens IgY level further increased after challenge infection. The study provides evidence that acquired resistance against A. galli in chickens leads to a significant yet incomplete protection against re-infection.",

author = "Tania Ferdushy and Schou, {T. W.} and Norup, {Liselotte Rothmann} and Dalgaard, {Tina S{\o}rensen} and Thamsborg, {Stig Milan} and Peter Nejsum and A. Permin and Juul-Madsen, {Helle Risdahl} and Kyvsgaard, {Niels Christian}",

year = "2014",

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doi = "10.1017/s0031182014000742",

language = "English",

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publisher = "Cambridge University Press",

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T1 - Acquisition of resistance after continuous infection with Ascaridia galli in chickens

AU - Ferdushy, Tania

AU - Schou, T. W.

AU - Norup, Liselotte Rothmann

AU - Dalgaard, Tina Sørensen

AU - Thamsborg, Stig Milan

AU - Nejsum, Peter

AU - Permin, A.

AU - Juul-Madsen, Helle Risdahl

AU - Kyvsgaard, Niels Christian

PY - 2014/10

Y1 - 2014/10

N2 - Acquired resistance against Ascaridia galli infection was studied in seventy-two 18-week-old white Leghorn chickens allocated to six groups (G1-G6). In order to understand the population dynamics following trickle-infection (100 eggs per chicken twice weekly), chickens of subgroups of G1 were necropsied 3 days after 1, 6 or 12 inoculations (G1A, G1B and G1C respectively), while G2-G4 were inoculated for 6 weeks. G2 was necropsied 4 weeks after the last inoculation. The number of established larvae increased initially (between G1A and G1B) but decreased after repeated inoculations (G1C, G2). G3, G4 and G5 were used to measure the efficacy of anthelminthic treatment and to monitor the acquisition of resistance following a challenge infection. At week 7 G3, G4 and G5 were treated with flubendazole for 7 days in the feed. Two weeks after treatment the chickens in G4 and G5 were challenged with 500 eggs. G6 was left as uninfected control. Necropsy at week 10 after first inoculation revealed a lower establishment rate, an impaired development and a more posterior localization of the larvae in G4 (trickle-infected-treated-challenged) compared with G5 (treated-challenged). IgY level in serum reached noticeable level at 14 dpi in G2 and G4 chickens, and in G4 chickens IgY level further increased after challenge infection. The study provides evidence that acquired resistance against A. galli in chickens leads to a significant yet incomplete protection against re-infection.

AB - Acquired resistance against Ascaridia galli infection was studied in seventy-two 18-week-old white Leghorn chickens allocated to six groups (G1-G6). In order to understand the population dynamics following trickle-infection (100 eggs per chicken twice weekly), chickens of subgroups of G1 were necropsied 3 days after 1, 6 or 12 inoculations (G1A, G1B and G1C respectively), while G2-G4 were inoculated for 6 weeks. G2 was necropsied 4 weeks after the last inoculation. The number of established larvae increased initially (between G1A and G1B) but decreased after repeated inoculations (G1C, G2). G3, G4 and G5 were used to measure the efficacy of anthelminthic treatment and to monitor the acquisition of resistance following a challenge infection. At week 7 G3, G4 and G5 were treated with flubendazole for 7 days in the feed. Two weeks after treatment the chickens in G4 and G5 were challenged with 500 eggs. G6 was left as uninfected control. Necropsy at week 10 after first inoculation revealed a lower establishment rate, an impaired development and a more posterior localization of the larvae in G4 (trickle-infected-treated-challenged) compared with G5 (treated-challenged). IgY level in serum reached noticeable level at 14 dpi in G2 and G4 chickens, and in G4 chickens IgY level further increased after challenge infection. The study provides evidence that acquired resistance against A. galli in chickens leads to a significant yet incomplete protection against re-infection.

U2 - 10.1017/s0031182014000742

DO - 10.1017/s0031182014000742

M3 - Journal article

C2 - 25003836

SN - 0031-1820

VL - 141

SP - 1603

EP - 1610

JO - Parasitology

JF - Parasitology

IS - 12

ER -

Acquisition of resistance after continuous infection with Ascaridia galli in chickens

Abstract

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