Accumulation of succinate controls activation of adipose tissue thermogenesis

Evanna L. Mills; Kerry A. Pierce; Mark P. Jedrychowski; Ryan Garrity; Sally Winther; Sara Vidoni; Takeshi Yoneshiro; Jessica B. Spinelli; Gina Z. Lu; Lawrence Kazak; Alexander S. Banks; Marcia C. Haigis; Shingo Kajimura; Michael P. Murphy; Steven P. Gygi; Clary B. Clish; Edward T. Chouchani

doi:10.1038/s41586-018-0353-2

Accumulation of succinate controls activation of adipose tissue thermogenesis

Evanna L. Mills, Kerry A. Pierce, Mark P. Jedrychowski, Ryan Garrity, Sally Winther, Sara Vidoni, Takeshi Yoneshiro, Jessica B. Spinelli, Gina Z. Lu, Lawrence Kazak, Alexander S. Banks, Marcia C. Haigis, Shingo Kajimura, Michael P. Murphy, Steven P. Gygi, Clary B. Clish, Edward T. Chouchani^*

^*Corresponding author for this work

136 Citations (Scopus)

Abstract

Thermogenesis by brown and beige adipose tissue, which requires activation by external stimuli, can counter metabolic disease¹. Thermogenic respiration is initiated by adipocyte lipolysis through cyclic AMP–protein kinase A signalling; this pathway has been subject to longstanding clinical investigation^2–4. Here we apply a comparative metabolomics approach and identify an independent metabolic pathway that controls acute activation of adipose tissue thermogenesis in vivo. We show that substantial and selective accumulation of the tricarboxylic acid cycle intermediate succinate is a metabolic signature of adipose tissue thermogenesis upon activation by exposure to cold. Succinate accumulation occurs independently of adrenergic signalling, and is sufficient to elevate thermogenic respiration in brown adipocytes. Selective accumulation of succinate may be driven by a capacity of brown adipocytes to sequester elevated circulating succinate. Furthermore, brown adipose tissue thermogenesis can be initiated by systemic administration of succinate in mice. Succinate from the extracellular milieu is rapidly metabolized by brown adipocytes, and its oxidation by succinate dehydrogenase is required for activation of thermogenesis. We identify a mechanism whereby succinate dehydrogenase-mediated oxidation of succinate initiates production of reactive oxygen species, and drives thermogenic respiration, whereas inhibition of succinate dehydrogenase supresses thermogenesis. Finally, we show that pharmacological elevation of circulating succinate drives UCP1-dependent thermogenesis by brown adipose tissue in vivo, which stimulates robust protection against diet-induced obesity and improves glucose tolerance. These findings reveal an unexpected mechanism for control of thermogenesis, using succinate as a systemically-derived thermogenic molecule.

Original language	English
Journal	Nature
Volume	560
Issue number	7716
Pages (from-to)	102-106
Number of pages	5
ISSN	0028-0836
DOIs	https://doi.org/10.1038/s41586-018-0353-2
Publication status	Published - 2018
Externally published	Yes

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Mills, E. L., Pierce, K. A., Jedrychowski, M. P., Garrity, R., Winther, S., Vidoni, S., Yoneshiro, T., Spinelli, J. B., Lu, G. Z., Kazak, L., Banks, A. S., Haigis, M. C., Kajimura, S., Murphy, M. P., Gygi, S. P., Clish, C. B., & Chouchani, E. T. (2018). Accumulation of succinate controls activation of adipose tissue thermogenesis. Nature, 560(7716), 102-106. https://doi.org/10.1038/s41586-018-0353-2

Mills, EL, Pierce, KA, Jedrychowski, MP, Garrity, R, Winther, S, Vidoni, S, Yoneshiro, T, Spinelli, JB, Lu, GZ, Kazak, L, Banks, AS, Haigis, MC, Kajimura, S, Murphy, MP, Gygi, SP, Clish, CB & Chouchani, ET 2018, 'Accumulation of succinate controls activation of adipose tissue thermogenesis', Nature, vol. 560, no. 7716, pp. 102-106. https://doi.org/10.1038/s41586-018-0353-2

@article{1856f2f999254ff799d3ed49024906f8,

title = "Accumulation of succinate controls activation of adipose tissue thermogenesis",

abstract = "Thermogenesis by brown and beige adipose tissue, which requires activation by external stimuli, can counter metabolic disease1. Thermogenic respiration is initiated by adipocyte lipolysis through cyclic AMP–protein kinase A signalling; this pathway has been subject to longstanding clinical investigation2–4. Here we apply a comparative metabolomics approach and identify an independent metabolic pathway that controls acute activation of adipose tissue thermogenesis in vivo. We show that substantial and selective accumulation of the tricarboxylic acid cycle intermediate succinate is a metabolic signature of adipose tissue thermogenesis upon activation by exposure to cold. Succinate accumulation occurs independently of adrenergic signalling, and is sufficient to elevate thermogenic respiration in brown adipocytes. Selective accumulation of succinate may be driven by a capacity of brown adipocytes to sequester elevated circulating succinate. Furthermore, brown adipose tissue thermogenesis can be initiated by systemic administration of succinate in mice. Succinate from the extracellular milieu is rapidly metabolized by brown adipocytes, and its oxidation by succinate dehydrogenase is required for activation of thermogenesis. We identify a mechanism whereby succinate dehydrogenase-mediated oxidation of succinate initiates production of reactive oxygen species, and drives thermogenic respiration, whereas inhibition of succinate dehydrogenase supresses thermogenesis. Finally, we show that pharmacological elevation of circulating succinate drives UCP1-dependent thermogenesis by brown adipose tissue in vivo, which stimulates robust protection against diet-induced obesity and improves glucose tolerance. These findings reveal an unexpected mechanism for control of thermogenesis, using succinate as a systemically-derived thermogenic molecule.",

author = "Mills, {Evanna L.} and Pierce, {Kerry A.} and Jedrychowski, {Mark P.} and Ryan Garrity and Sally Winther and Sara Vidoni and Takeshi Yoneshiro and Spinelli, {Jessica B.} and Lu, {Gina Z.} and Lawrence Kazak and Banks, {Alexander S.} and Haigis, {Marcia C.} and Shingo Kajimura and Murphy, {Michael P.} and Gygi, {Steven P.} and Clish, {Clary B.} and Chouchani, {Edward T.}",

year = "2018",

doi = "10.1038/s41586-018-0353-2",

language = "English",

volume = "560",

pages = "102--106",

journal = "Nature",

issn = "0028-0836",

publisher = "nature publishing group",

number = "7716",

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T1 - Accumulation of succinate controls activation of adipose tissue thermogenesis

AU - Mills, Evanna L.

AU - Pierce, Kerry A.

AU - Jedrychowski, Mark P.

AU - Garrity, Ryan

AU - Winther, Sally

AU - Vidoni, Sara

AU - Yoneshiro, Takeshi

AU - Spinelli, Jessica B.

AU - Lu, Gina Z.

AU - Kazak, Lawrence

AU - Banks, Alexander S.

AU - Haigis, Marcia C.

AU - Kajimura, Shingo

AU - Murphy, Michael P.

AU - Gygi, Steven P.

AU - Clish, Clary B.

AU - Chouchani, Edward T.

PY - 2018

Y1 - 2018

N2 - Thermogenesis by brown and beige adipose tissue, which requires activation by external stimuli, can counter metabolic disease1. Thermogenic respiration is initiated by adipocyte lipolysis through cyclic AMP–protein kinase A signalling; this pathway has been subject to longstanding clinical investigation2–4. Here we apply a comparative metabolomics approach and identify an independent metabolic pathway that controls acute activation of adipose tissue thermogenesis in vivo. We show that substantial and selective accumulation of the tricarboxylic acid cycle intermediate succinate is a metabolic signature of adipose tissue thermogenesis upon activation by exposure to cold. Succinate accumulation occurs independently of adrenergic signalling, and is sufficient to elevate thermogenic respiration in brown adipocytes. Selective accumulation of succinate may be driven by a capacity of brown adipocytes to sequester elevated circulating succinate. Furthermore, brown adipose tissue thermogenesis can be initiated by systemic administration of succinate in mice. Succinate from the extracellular milieu is rapidly metabolized by brown adipocytes, and its oxidation by succinate dehydrogenase is required for activation of thermogenesis. We identify a mechanism whereby succinate dehydrogenase-mediated oxidation of succinate initiates production of reactive oxygen species, and drives thermogenic respiration, whereas inhibition of succinate dehydrogenase supresses thermogenesis. Finally, we show that pharmacological elevation of circulating succinate drives UCP1-dependent thermogenesis by brown adipose tissue in vivo, which stimulates robust protection against diet-induced obesity and improves glucose tolerance. These findings reveal an unexpected mechanism for control of thermogenesis, using succinate as a systemically-derived thermogenic molecule.

AB - Thermogenesis by brown and beige adipose tissue, which requires activation by external stimuli, can counter metabolic disease1. Thermogenic respiration is initiated by adipocyte lipolysis through cyclic AMP–protein kinase A signalling; this pathway has been subject to longstanding clinical investigation2–4. Here we apply a comparative metabolomics approach and identify an independent metabolic pathway that controls acute activation of adipose tissue thermogenesis in vivo. We show that substantial and selective accumulation of the tricarboxylic acid cycle intermediate succinate is a metabolic signature of adipose tissue thermogenesis upon activation by exposure to cold. Succinate accumulation occurs independently of adrenergic signalling, and is sufficient to elevate thermogenic respiration in brown adipocytes. Selective accumulation of succinate may be driven by a capacity of brown adipocytes to sequester elevated circulating succinate. Furthermore, brown adipose tissue thermogenesis can be initiated by systemic administration of succinate in mice. Succinate from the extracellular milieu is rapidly metabolized by brown adipocytes, and its oxidation by succinate dehydrogenase is required for activation of thermogenesis. We identify a mechanism whereby succinate dehydrogenase-mediated oxidation of succinate initiates production of reactive oxygen species, and drives thermogenic respiration, whereas inhibition of succinate dehydrogenase supresses thermogenesis. Finally, we show that pharmacological elevation of circulating succinate drives UCP1-dependent thermogenesis by brown adipose tissue in vivo, which stimulates robust protection against diet-induced obesity and improves glucose tolerance. These findings reveal an unexpected mechanism for control of thermogenesis, using succinate as a systemically-derived thermogenic molecule.

U2 - 10.1038/s41586-018-0353-2

DO - 10.1038/s41586-018-0353-2

M3 - Letter

C2 - 30022159

AN - SCOPUS:85050992801

SN - 0028-0836

VL - 560

SP - 102

EP - 106

JO - Nature

JF - Nature

IS - 7716

ER -

Accumulation of succinate controls activation of adipose tissue thermogenesis

Abstract

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