TY - JOUR
T1 - Absorption and initial metabolism of 75Se-l-selenomethionine
T2 - a kinetic model based on dynamic scintigraphic data
AU - Grosse Ruse, Mareile
AU - Søndergaard, Lasse R.
AU - Ditlevsen, Susanne
AU - Damgaard, Morten
AU - Fuglsang, Stefan
AU - Ottesen, Johnny T.
AU - Madsen, Jan L.
PY - 2015/10/5
Y1 - 2015/10/5
N2 - Selenomethionine (SeMet) is an important organic nutritional source of
Se, but the uptake and metabolism of SeMet are poorly characterised in
humans. Dynamic gamma camera images of the abdominal region were
acquired from eight healthy young men after the ingestion of radioactive
75Se-l-SeMet (75Se-SeMet). Scanning started simultaneously to the ingestion of 75Se-SeMet
and lasted 120 min. We generated time-activity curves from
two-dimensional regions of interest in the stomach, small intestine and
liver. During scanning, blood samples were collected at 10-min intervals
to generate plasma time-activity curves. A four-compartment model,
augmented with a delay between the liver and plasma, was fitted to
individual participants’ data. The mean rate constant for 75Se-SeMet transport was 2·63 h–1 from the stomach to the small intestine, 13·2 h–1 from the small intestine to the liver, 0·261 h–1 from the liver to the plasma and 0·267 h–1
from the stomach to the plasma. The delay in the liver was 0·714 h.
Gamma camera imaging provides data for use in compartmental modelling of
75Se-SeMet absorption and metabolism in humans. In clinical
settings, the obtained rate constants and the delay in the liver may be
useful variables for quantifying reduced intestinal absorption capacity
or liver function.
AB - Selenomethionine (SeMet) is an important organic nutritional source of
Se, but the uptake and metabolism of SeMet are poorly characterised in
humans. Dynamic gamma camera images of the abdominal region were
acquired from eight healthy young men after the ingestion of radioactive
75Se-l-SeMet (75Se-SeMet). Scanning started simultaneously to the ingestion of 75Se-SeMet
and lasted 120 min. We generated time-activity curves from
two-dimensional regions of interest in the stomach, small intestine and
liver. During scanning, blood samples were collected at 10-min intervals
to generate plasma time-activity curves. A four-compartment model,
augmented with a delay between the liver and plasma, was fitted to
individual participants’ data. The mean rate constant for 75Se-SeMet transport was 2·63 h–1 from the stomach to the small intestine, 13·2 h–1 from the small intestine to the liver, 0·261 h–1 from the liver to the plasma and 0·267 h–1
from the stomach to the plasma. The delay in the liver was 0·714 h.
Gamma camera imaging provides data for use in compartmental modelling of
75Se-SeMet absorption and metabolism in humans. In clinical
settings, the obtained rate constants and the delay in the liver may be
useful variables for quantifying reduced intestinal absorption capacity
or liver function.
U2 - 10.1017/s000711451500344x
DO - 10.1017/s000711451500344x
M3 - Journal article
SN - 0007-1145
VL - 114
SP - 1718
EP - 1723
JO - British Journal of Nutrition
JF - British Journal of Nutrition
IS - 10
ER -