ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis

Rozenn Le Hir; Clement Sorin; Dipankar Chakraborti; Thomas Moritz; Hubert Schaller; Frederique Tellier; Stephanie Robert; Halima Morin; Laszlo Bako; Catherine Bellini

ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis

Rozenn Le Hir, Clement Sorin, Dipankar Chakraborti, Thomas Moritz, Hubert Schaller, Frederique Tellier, Stephanie Robert, Halima Morin, Laszlo Bako, Catherine Bellini

40 Citations (Scopus)

Abstract

In order to obtain insights into the regulatory pathways controlling phloem development, we characterized three genes encoding membrane proteins from the G sub-family of ABC transporters (ABCG9, ABCG11 and ABCG14), whose expression in the phloem has been confirmed. Mutations in the genes encoding these dimerizing 'half transporters' are semi-dominant and result in vascular patterning defects in cotyledons and the floral stem. Co-immunoprecipitation and bimolecular fluorescence complementation experiments demonstrated that these proteins dimerize, either by flexible pairing (ABCG11 and ABCG9) or by forming strict heterodimers (ABCG14). In addition, metabolome analyses and measurement of sterol ester contents in the mutants suggested that ABCG9, ABCG11 and ABCG14 are involved in lipid/sterol homeostasis regulation. Our results show that these three ABCG genes are required for proper vascular development in Arabidopsis thaliana.

Original language	English
Journal	Plant Journal
Volume	76
Issue number	5
Pages (from-to)	811-824
Number of pages	14
ISSN	0960-7412
Publication status	Published - Dec 2013
Externally published	Yes

Cite this

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title = "ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis",

abstract = "In order to obtain insights into the regulatory pathways controlling phloem development, we characterized three genes encoding membrane proteins from the G sub-family of ABC transporters (ABCG9, ABCG11 and ABCG14), whose expression in the phloem has been confirmed. Mutations in the genes encoding these dimerizing 'half transporters' are semi-dominant and result in vascular patterning defects in cotyledons and the floral stem. Co-immunoprecipitation and bimolecular fluorescence complementation experiments demonstrated that these proteins dimerize, either by flexible pairing (ABCG11 and ABCG9) or by forming strict heterodimers (ABCG14). In addition, metabolome analyses and measurement of sterol ester contents in the mutants suggested that ABCG9, ABCG11 and ABCG14 are involved in lipid/sterol homeostasis regulation. Our results show that these three ABCG genes are required for proper vascular development in Arabidopsis thaliana.",

author = "{Le Hir}, Rozenn and Clement Sorin and Dipankar Chakraborti and Thomas Moritz and Hubert Schaller and Frederique Tellier and Stephanie Robert and Halima Morin and Laszlo Bako and Catherine Bellini",

year = "2013",

month = dec,

language = "English",

volume = "76",

pages = "811--824",

journal = "Plant Journal",

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TY - JOUR

T1 - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis

AU - Le Hir, Rozenn

AU - Sorin, Clement

AU - Chakraborti, Dipankar

AU - Moritz, Thomas

AU - Schaller, Hubert

AU - Tellier, Frederique

AU - Robert, Stephanie

AU - Morin, Halima

AU - Bako, Laszlo

AU - Bellini, Catherine

PY - 2013/12

Y1 - 2013/12

N2 - In order to obtain insights into the regulatory pathways controlling phloem development, we characterized three genes encoding membrane proteins from the G sub-family of ABC transporters (ABCG9, ABCG11 and ABCG14), whose expression in the phloem has been confirmed. Mutations in the genes encoding these dimerizing 'half transporters' are semi-dominant and result in vascular patterning defects in cotyledons and the floral stem. Co-immunoprecipitation and bimolecular fluorescence complementation experiments demonstrated that these proteins dimerize, either by flexible pairing (ABCG11 and ABCG9) or by forming strict heterodimers (ABCG14). In addition, metabolome analyses and measurement of sterol ester contents in the mutants suggested that ABCG9, ABCG11 and ABCG14 are involved in lipid/sterol homeostasis regulation. Our results show that these three ABCG genes are required for proper vascular development in Arabidopsis thaliana.

AB - In order to obtain insights into the regulatory pathways controlling phloem development, we characterized three genes encoding membrane proteins from the G sub-family of ABC transporters (ABCG9, ABCG11 and ABCG14), whose expression in the phloem has been confirmed. Mutations in the genes encoding these dimerizing 'half transporters' are semi-dominant and result in vascular patterning defects in cotyledons and the floral stem. Co-immunoprecipitation and bimolecular fluorescence complementation experiments demonstrated that these proteins dimerize, either by flexible pairing (ABCG11 and ABCG9) or by forming strict heterodimers (ABCG14). In addition, metabolome analyses and measurement of sterol ester contents in the mutants suggested that ABCG9, ABCG11 and ABCG14 are involved in lipid/sterol homeostasis regulation. Our results show that these three ABCG genes are required for proper vascular development in Arabidopsis thaliana.

M3 - Journal article

SN - 0960-7412

VL - 76

SP - 811

EP - 824

JO - Plant Journal

JF - Plant Journal

IS - 5

ER -

ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis

Abstract

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