AAA ATPase p529 of Acidianus two-tailed virus ATV and host receptor recognition

TY - JOUR

T1 - AAA ATPase p529 of Acidianus two-tailed virus ATV and host receptor recognition

AU - Erdmann, Susanne

AU - Scheele, Urte

AU - Garrett, Roger Antony

PY - 2011/12/5

Y1 - 2011/12/5

N2 - The two structural domains of p529, a predicted AAA ATPase of Acidianus two-tailed virus (ATV), were expressed and purified. The N-terminal domain was demonstrated by loss-of-function mutations to carry ATPase activity with a temperature optimum of 60°C. This domain also showed DNA binding activity that was stronger for the whole protein and was weakened in the presence of ATP. The C-terminal domain exhibits Mg 2+-dependent endonuclease activity that was eliminated by site-directed mutagenesis at a conserved catalytic PD. .D/ExK motif. p529 pull-down experiments with cell extracts of Sulfolobus solfataricus demonstrated a specific interaction with Sso1273, corresponding to OppA Ss, an N-linked glycoprotein that specifically binds oligopeptides. The sso1273 gene lies in an operon encoding an oligopeptide/dipeptide ABC transporter system. It is proposed that p529 is involved in ATV-host cell receptor recognition and possibly the endonuclease activity is required for cleavage of the circular viral DNA prior to cell entry.

AB - The two structural domains of p529, a predicted AAA ATPase of Acidianus two-tailed virus (ATV), were expressed and purified. The N-terminal domain was demonstrated by loss-of-function mutations to carry ATPase activity with a temperature optimum of 60°C. This domain also showed DNA binding activity that was stronger for the whole protein and was weakened in the presence of ATP. The C-terminal domain exhibits Mg 2+-dependent endonuclease activity that was eliminated by site-directed mutagenesis at a conserved catalytic PD. .D/ExK motif. p529 pull-down experiments with cell extracts of Sulfolobus solfataricus demonstrated a specific interaction with Sso1273, corresponding to OppA Ss, an N-linked glycoprotein that specifically binds oligopeptides. The sso1273 gene lies in an operon encoding an oligopeptide/dipeptide ABC transporter system. It is proposed that p529 is involved in ATV-host cell receptor recognition and possibly the endonuclease activity is required for cleavage of the circular viral DNA prior to cell entry.

U2 - 10.1016/j.virol.2011.08.029

DO - 10.1016/j.virol.2011.08.029

M3 - Journal article

C2 - 21982819

SN - 0042-6822

VL - 421

SP - 61

EP - 66

JO - Virology

JF - Virology

IS - 1

ER -