A volumetric screening procedure for the Göttingen minipig brain.

J Jelsing; Aage K. Olsen; P Cumming; A Gjedde; A K Hansen; S Arnfred; R Hemmingsen; Bente Pakkenberg

doi:10.1007/s00221-004-2026-7

A volumetric screening procedure for the Göttingen minipig brain.

J Jelsing, Aage K. Olsen, P Cumming, A Gjedde, A K Hansen, S Arnfred, R Hemmingsen, Bente Pakkenberg

7 Citations (Scopus)

Abstract

A screening procedure was developed to provide quantitative estimates of structural parameters, regional volumes and neuron number, in a neurotoxicologic study of the Göttingen minipig brain. The study material consisted of normal controls and brains collected from young minipigs which had been exposed in utero to the mitotic inhibitor methylazoxymethanol acetate (MAM). Based on stereological principles and systematic sampling techniques, volumetric data from pre-selected regions of the pig brain was obtained using Cavalieri's principles and point-counting. Secondarily, estimates of total hemispheric neocortical cell numbers were obtained from pre-selected groups to test the potential effect of MAM on neuron number. No significant differences were observed in volume of the pre-selected regions of MAM intoxicated pigs nor in estimates of total neocortical neuron number.

Original language	English
Journal	Experimental Brain Research
Volume	162
Issue number	4
Pages (from-to)	428-35
Number of pages	7
ISSN	0014-4819
DOIs	https://doi.org/10.1007/s00221-004-2026-7
Publication status	Published - 2005
Externally published	Yes

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title = "A volumetric screening procedure for the G{\"o}ttingen minipig brain.",

abstract = "A screening procedure was developed to provide quantitative estimates of structural parameters, regional volumes and neuron number, in a neurotoxicologic study of the G{\"o}ttingen minipig brain. The study material consisted of normal controls and brains collected from young minipigs which had been exposed in utero to the mitotic inhibitor methylazoxymethanol acetate (MAM). Based on stereological principles and systematic sampling techniques, volumetric data from pre-selected regions of the pig brain was obtained using Cavalieri's principles and point-counting. Secondarily, estimates of total hemispheric neocortical cell numbers were obtained from pre-selected groups to test the potential effect of MAM on neuron number. No significant differences were observed in volume of the pre-selected regions of MAM intoxicated pigs nor in estimates of total neocortical neuron number.",

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year = "2005",

doi = "10.1007/s00221-004-2026-7",

language = "English",

volume = "162",

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journal = "Experimental Brain Research",

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T1 - A volumetric screening procedure for the Göttingen minipig brain.

AU - Jelsing, J

AU - Olsen, Aage K.

AU - Cumming, P

AU - Gjedde, A

AU - Hansen, A K

AU - Arnfred, S

AU - Hemmingsen, R

AU - Pakkenberg, Bente

PY - 2005

Y1 - 2005

N2 - A screening procedure was developed to provide quantitative estimates of structural parameters, regional volumes and neuron number, in a neurotoxicologic study of the Göttingen minipig brain. The study material consisted of normal controls and brains collected from young minipigs which had been exposed in utero to the mitotic inhibitor methylazoxymethanol acetate (MAM). Based on stereological principles and systematic sampling techniques, volumetric data from pre-selected regions of the pig brain was obtained using Cavalieri's principles and point-counting. Secondarily, estimates of total hemispheric neocortical cell numbers were obtained from pre-selected groups to test the potential effect of MAM on neuron number. No significant differences were observed in volume of the pre-selected regions of MAM intoxicated pigs nor in estimates of total neocortical neuron number.

AB - A screening procedure was developed to provide quantitative estimates of structural parameters, regional volumes and neuron number, in a neurotoxicologic study of the Göttingen minipig brain. The study material consisted of normal controls and brains collected from young minipigs which had been exposed in utero to the mitotic inhibitor methylazoxymethanol acetate (MAM). Based on stereological principles and systematic sampling techniques, volumetric data from pre-selected regions of the pig brain was obtained using Cavalieri's principles and point-counting. Secondarily, estimates of total hemispheric neocortical cell numbers were obtained from pre-selected groups to test the potential effect of MAM on neuron number. No significant differences were observed in volume of the pre-selected regions of MAM intoxicated pigs nor in estimates of total neocortical neuron number.

U2 - 10.1007/s00221-004-2026-7

DO - 10.1007/s00221-004-2026-7

M3 - Journal article

C2 - 15668795

SN - 0014-4819

VL - 162

SP - 428

EP - 435

JO - Experimental Brain Research

JF - Experimental Brain Research

IS - 4

ER -

A volumetric screening procedure for the Göttingen minipig brain.

Abstract

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