A two-week reduction of ambulatory activity attenuates peripheral insulin sensitivity

Rikke Krogh-Madsen; John P Thyfault; Christa Broholm; Ole Hartvig Mortensen; Rasmus Huan Olsen; Remi Mounier; Peter Plomgaard; Gerrit van Hall; Frank W Booth; Bente Klarlund Pedersen

doi:10.1152/japplphysiol.00977.2009

A two-week reduction of ambulatory activity attenuates peripheral insulin sensitivity

Rikke Krogh-Madsen, John P Thyfault, Christa Broholm, Ole Hartvig Mortensen, Rasmus Huan Olsen, Remi Mounier, Peter Plomgaard, Gerrit van Hall, Frank W Booth, Bente Klarlund Pedersen

166 Citations (Scopus)

Abstract

US adults take between ~2,000 to ~12,000 steps per day, a wide range of ambulatory activity, that at the low range could increase risk for developing chronic metabolic diseases. Dramatic reductions in physical activity induce insulin resistance; however it is uncertain if and how low ambulatory activity would influence peripheral insulin sensitivity. We aimed to explore if healthy, non-exercising subjects who went from a normal to a low level of ambulatory activity for two weeks would display metabolic alterations including reduced peripheral insulin sensitivity. -To do this, ten healthy young men decreased their daily activity level from a mean of 10,501 (+/- 808) to 1,344 (+/- 33) steps/day for 2 weeks. Hyperinsulinemic-euglycemic clamps with stable isotopes and muscle biopsies, maximal oxygen consumption (VO2max) tests, and blood samples were performed pre and post intervention.A reduced number of daily steps induced a significant reduction of 17% in the glucose infusion rate (GIR) during the clamp. This reduction was due to a decline in peripheral insulin sensitivity with no effect on hepatic endogenous glucose production. The insulin-stimulated ratio of pAkt(thr308)/total Akt decreased after step reduction, with a post hoc analysis revealing the most pronounced effect after 4 h of insulin infusion. In addition, the two-week period induced a 7% decline in VO2max (ml/min; cardiovascular fitness). Lean mass of legs, but not arms and truck, decreased concurrently. Taken together, one possible biological cause for the public health problem of type 2 diabetes has been identified. Reduced ambulatory activity for two weeks in healthy, non-exercising young men significantly reduced peripheral insulin sensitivity, cardiovascular fitness, and lean leg mass. Key words: Inactivity, Insulin resistance, Clamp, Insulin signalling.

Original language	English
Journal	Journal of Applied Physiology
ISSN	8750-7587
DOIs	https://doi.org/10.1152/japplphysiol.00977.2009
Publication status	Published - 2009

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@article{522e695035aa11df8ed1000ea68e967b,

title = "A two-week reduction of ambulatory activity attenuates peripheral insulin sensitivity",

abstract = "US adults take between ~2,000 to ~12,000 steps per day, a wide range of ambulatory activity, that at the low range could increase risk for developing chronic metabolic diseases. Dramatic reductions in physical activity induce insulin resistance; however it is uncertain if and how low ambulatory activity would influence peripheral insulin sensitivity. We aimed to explore if healthy, non-exercising subjects who went from a normal to a low level of ambulatory activity for two weeks would display metabolic alterations including reduced peripheral insulin sensitivity. -To do this, ten healthy young men decreased their daily activity level from a mean of 10,501 (+/- 808) to 1,344 (+/- 33) steps/day for 2 weeks. Hyperinsulinemic-euglycemic clamps with stable isotopes and muscle biopsies, maximal oxygen consumption (VO2max) tests, and blood samples were performed pre and post intervention.A reduced number of daily steps induced a significant reduction of 17% in the glucose infusion rate (GIR) during the clamp. This reduction was due to a decline in peripheral insulin sensitivity with no effect on hepatic endogenous glucose production. The insulin-stimulated ratio of pAkt(thr308)/total Akt decreased after step reduction, with a post hoc analysis revealing the most pronounced effect after 4 h of insulin infusion. In addition, the two-week period induced a 7% decline in VO2max (ml/min; cardiovascular fitness). Lean mass of legs, but not arms and truck, decreased concurrently. Taken together, one possible biological cause for the public health problem of type 2 diabetes has been identified. Reduced ambulatory activity for two weeks in healthy, non-exercising young men significantly reduced peripheral insulin sensitivity, cardiovascular fitness, and lean leg mass. Key words: Inactivity, Insulin resistance, Clamp, Insulin signalling.",

author = "Rikke Krogh-Madsen and Thyfault, {John P} and Christa Broholm and Mortensen, {Ole Hartvig} and Olsen, {Rasmus Huan} and Remi Mounier and Peter Plomgaard and Hall, {Gerrit van} and Booth, {Frank W} and Pedersen, {Bente Klarlund}",

year = "2009",

doi = "10.1152/japplphysiol.00977.2009",

language = "English",

journal = "Journal of Applied Physiology",

issn = "8750-7587",

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TY - JOUR

T1 - A two-week reduction of ambulatory activity attenuates peripheral insulin sensitivity

AU - Krogh-Madsen, Rikke

AU - Thyfault, John P

AU - Broholm, Christa

AU - Mortensen, Ole Hartvig

AU - Olsen, Rasmus Huan

AU - Mounier, Remi

AU - Plomgaard, Peter

AU - Hall, Gerrit van

AU - Booth, Frank W

AU - Pedersen, Bente Klarlund

PY - 2009

Y1 - 2009

N2 - US adults take between ~2,000 to ~12,000 steps per day, a wide range of ambulatory activity, that at the low range could increase risk for developing chronic metabolic diseases. Dramatic reductions in physical activity induce insulin resistance; however it is uncertain if and how low ambulatory activity would influence peripheral insulin sensitivity. We aimed to explore if healthy, non-exercising subjects who went from a normal to a low level of ambulatory activity for two weeks would display metabolic alterations including reduced peripheral insulin sensitivity. -To do this, ten healthy young men decreased their daily activity level from a mean of 10,501 (+/- 808) to 1,344 (+/- 33) steps/day for 2 weeks. Hyperinsulinemic-euglycemic clamps with stable isotopes and muscle biopsies, maximal oxygen consumption (VO2max) tests, and blood samples were performed pre and post intervention.A reduced number of daily steps induced a significant reduction of 17% in the glucose infusion rate (GIR) during the clamp. This reduction was due to a decline in peripheral insulin sensitivity with no effect on hepatic endogenous glucose production. The insulin-stimulated ratio of pAkt(thr308)/total Akt decreased after step reduction, with a post hoc analysis revealing the most pronounced effect after 4 h of insulin infusion. In addition, the two-week period induced a 7% decline in VO2max (ml/min; cardiovascular fitness). Lean mass of legs, but not arms and truck, decreased concurrently. Taken together, one possible biological cause for the public health problem of type 2 diabetes has been identified. Reduced ambulatory activity for two weeks in healthy, non-exercising young men significantly reduced peripheral insulin sensitivity, cardiovascular fitness, and lean leg mass. Key words: Inactivity, Insulin resistance, Clamp, Insulin signalling.

AB - US adults take between ~2,000 to ~12,000 steps per day, a wide range of ambulatory activity, that at the low range could increase risk for developing chronic metabolic diseases. Dramatic reductions in physical activity induce insulin resistance; however it is uncertain if and how low ambulatory activity would influence peripheral insulin sensitivity. We aimed to explore if healthy, non-exercising subjects who went from a normal to a low level of ambulatory activity for two weeks would display metabolic alterations including reduced peripheral insulin sensitivity. -To do this, ten healthy young men decreased their daily activity level from a mean of 10,501 (+/- 808) to 1,344 (+/- 33) steps/day for 2 weeks. Hyperinsulinemic-euglycemic clamps with stable isotopes and muscle biopsies, maximal oxygen consumption (VO2max) tests, and blood samples were performed pre and post intervention.A reduced number of daily steps induced a significant reduction of 17% in the glucose infusion rate (GIR) during the clamp. This reduction was due to a decline in peripheral insulin sensitivity with no effect on hepatic endogenous glucose production. The insulin-stimulated ratio of pAkt(thr308)/total Akt decreased after step reduction, with a post hoc analysis revealing the most pronounced effect after 4 h of insulin infusion. In addition, the two-week period induced a 7% decline in VO2max (ml/min; cardiovascular fitness). Lean mass of legs, but not arms and truck, decreased concurrently. Taken together, one possible biological cause for the public health problem of type 2 diabetes has been identified. Reduced ambulatory activity for two weeks in healthy, non-exercising young men significantly reduced peripheral insulin sensitivity, cardiovascular fitness, and lean leg mass. Key words: Inactivity, Insulin resistance, Clamp, Insulin signalling.

U2 - 10.1152/japplphysiol.00977.2009

DO - 10.1152/japplphysiol.00977.2009

M3 - Journal article

C2 - 20044474

SN - 8750-7587

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

ER -

A two-week reduction of ambulatory activity attenuates peripheral insulin sensitivity

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