A transgenic mouse marking live replicating cells reveals in vivo transcriptional program of proliferation

TY - JOUR

T1 - A transgenic mouse marking live replicating cells reveals in vivo transcriptional program of proliferation

AU - Klochendler, Agnes

AU - Weinberg-Corem, Noa

AU - Moran, Maya

AU - Swisa, Avital

AU - Pochet, Nathalie

AU - Savova, Virginia

AU - Vikeså, Jonas

AU - Van de Peer, Yves

AU - Brandeis, Michael

AU - Regev, Aviv

AU - Nielsen, Finn Cilius

AU - Dor, Yuval

AU - Eden, Amir

N1 - Copyright © 2012 Elsevier Inc. All rights reserved.

PY - 2012/10/16

Y1 - 2012/10/16

N2 - Most adult mammalian tissues are quiescent, with rare cell divisions serving to maintain homeostasis. At present, the isolation and study of replicating cells from their in vivo niche typically involves immunostaining for intracellular markers of proliferation, causing the loss of sensitive biological material. We describe a transgenic mouse strain, expressing a CyclinB1-GFP fusion reporter, that marks replicating cells in the S/G2/M phases of the cell cycle. Using flow cytometry, we isolate live replicating cells from the liver and compare their transcriptome to that of quiescent cells to reveal gene expression programs associated with cell proliferation in vivo. We find that replicating hepatocytes have reduced expression of genes characteristic of liver differentiation. This reporter system provides a powerful platform for gene expression and metabolic and functional studies of replicating cells in their in vivo niche.

AB - Most adult mammalian tissues are quiescent, with rare cell divisions serving to maintain homeostasis. At present, the isolation and study of replicating cells from their in vivo niche typically involves immunostaining for intracellular markers of proliferation, causing the loss of sensitive biological material. We describe a transgenic mouse strain, expressing a CyclinB1-GFP fusion reporter, that marks replicating cells in the S/G2/M phases of the cell cycle. Using flow cytometry, we isolate live replicating cells from the liver and compare their transcriptome to that of quiescent cells to reveal gene expression programs associated with cell proliferation in vivo. We find that replicating hepatocytes have reduced expression of genes characteristic of liver differentiation. This reporter system provides a powerful platform for gene expression and metabolic and functional studies of replicating cells in their in vivo niche.

U2 - 10.1016/j.devcel.2012.08.009

DO - 10.1016/j.devcel.2012.08.009

M3 - Journal article

C2 - 23000141

SN - 1534-5807

VL - 23

SP - 681

EP - 690

JO - Developmental Cell

JF - Developmental Cell

IS - 4

ER -