A single rainbow trout cobalamin-binding protein stands in for three human binders

TY - JOUR

T1 - A single rainbow trout cobalamin-binding protein stands in for three human binders

AU - Greibe, Eva

AU - Fedosov, Sergey

AU - Sorensen, Boe S

AU - Højrup, Peter

AU - Poulsen, Steen Seier

AU - Nexo, Ebba

PY - 2012/9/28

Y1 - 2012/9/28

N2 - Cobalamin uptake and transport in mammals are mediated by three cobalamin-binding proteins: haptocorrin, intrinsic factor, and transcobalamin. The nature of cobalamin-binding proteins in lower vertebrates remains to be elucidated. The aim of this study was to characterize the cobalamin-binding proteins of the rainbow trout (Oncorhynchus mykiss) and to compare their properties with those of the three human cobalamin-binding proteins. High cobalamin-binding capacity was found in trout stomach (210 pmol/g), roe (400 pmol/g), roe fluid (390 nmol/liter), and plasma (2500 nmol/liter). In all cases, it appeared to be the same protein based on analysis of partial sequences and immunological responses. The trout cobalamin-binding protein was purified from roe fluid, sequenced, and further characterized. Like haptocorrin, the trout cobalamin-binding protein was stable at low pH and had a high binding affinity for the cobalamin analog cobinamide. Like haptocorrin and transcobalamin, the trout cobalamin-binding protein was present in plasma and recognized ligands with altered nucleotide moiety. Like intrinsic factors, the trout cobalamin-binding protein was present in the stomach and resisted degradation by trypsin and chymotrypsin. It also resembled intrinsic factor in the composition of conserved residues in the primary cobalamin-binding site in the C terminus. The trout cobalamin-binding protein was glycosylated and displayed spectral properties comparable with those of haptocorrin and intrinsic factor. In conclusion, only one soluble cobalamin-binding protein was identified in the rainbow trout, a protein that structurally behaves like an intermediate between the three human cobalamin-binding proteins.

AB - Cobalamin uptake and transport in mammals are mediated by three cobalamin-binding proteins: haptocorrin, intrinsic factor, and transcobalamin. The nature of cobalamin-binding proteins in lower vertebrates remains to be elucidated. The aim of this study was to characterize the cobalamin-binding proteins of the rainbow trout (Oncorhynchus mykiss) and to compare their properties with those of the three human cobalamin-binding proteins. High cobalamin-binding capacity was found in trout stomach (210 pmol/g), roe (400 pmol/g), roe fluid (390 nmol/liter), and plasma (2500 nmol/liter). In all cases, it appeared to be the same protein based on analysis of partial sequences and immunological responses. The trout cobalamin-binding protein was purified from roe fluid, sequenced, and further characterized. Like haptocorrin, the trout cobalamin-binding protein was stable at low pH and had a high binding affinity for the cobalamin analog cobinamide. Like haptocorrin and transcobalamin, the trout cobalamin-binding protein was present in plasma and recognized ligands with altered nucleotide moiety. Like intrinsic factors, the trout cobalamin-binding protein was present in the stomach and resisted degradation by trypsin and chymotrypsin. It also resembled intrinsic factor in the composition of conserved residues in the primary cobalamin-binding site in the C terminus. The trout cobalamin-binding protein was glycosylated and displayed spectral properties comparable with those of haptocorrin and intrinsic factor. In conclusion, only one soluble cobalamin-binding protein was identified in the rainbow trout, a protein that structurally behaves like an intermediate between the three human cobalamin-binding proteins.

KW - Amino Acid Sequence

KW - Animals

KW - Concanavalin A

KW - Gene Expression Regulation

KW - Glycosylation

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Intrinsic Factor

KW - Models, Animal

KW - Molecular Sequence Data

KW - Oncorhynchus mykiss

KW - Phylogeny

KW - Protein Binding

KW - Protein Structure, Tertiary

KW - Sequence Homology, Amino Acid

KW - Species Specificity

KW - Transcobalamins

KW - Vitamin B Complex

U2 - 10.1074/jbc.M112.398016

DO - 10.1074/jbc.M112.398016

M3 - Journal article

C2 - 22872637

SN - 0021-9258

VL - 287

SP - 33917

EP - 33925

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 40

ER -