A single gene target of an ETS-family transcription factor determines neuronal CO2-chemosensitivity

Julia P Brandt; Sonya Aziz-Zaman; Vaida Juozaityte; Luis A Martinez-Velazquez; Jakob Gramstrup Petersen; Roger David John Pocock; Niels Ringstad

doi:10.1371/journal.pone.0034014

A single gene target of an ETS-family transcription factor determines neuronal CO2-chemosensitivity

Julia P Brandt, Sonya Aziz-Zaman, Vaida Juozaityte, Luis A Martinez-Velazquez, Jakob Gramstrup Petersen, Roger David John Pocock, Niels Ringstad

24 Citations (Scopus)

Abstract

Many animals possess neurons specialized for the detection of carbon dioxide (CO(2)), which acts as a cue to elicit behavioral responses and is also an internally generated product of respiration that regulates animal physiology. In many organisms how such neurons detect CO(2) is poorly understood. We report here a mechanism that endows C. elegans neurons with the ability to detect CO(2). The ETS-5 transcription factor is necessary for the specification of CO(2)-sensing BAG neurons. Expression of a single ETS-5 target gene, gcy-9, which encodes a receptor-type guanylate cyclase, is sufficient to bypass a requirement for ets-5 in CO(2)-detection and transforms neurons into CO(2)-sensing neurons. Because ETS-5 and GCY-9 are members of gene families that are conserved between nematodes and vertebrates, a similar mechanism might act in the specification of CO(2)-sensing neurons in other phyla.

Original language	English
Journal	PLOS ONE
Volume	7
Issue number	3
Pages (from-to)	e34014
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0034014
Publication status	Published - 29 Mar 2012

Keywords

Alleles
Animals
Behavior, Animal
Binding Sites
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Carbon Dioxide
Gene Deletion
Gene Expression Regulation
Guanylate Cyclase
Microscopy, Fluorescence
Mutation
Neurons
Plasmids
Proto-Oncogene Proteins c-ets
Receptors, Guanylate Cyclase-Coupled
Sensory Receptor Cells

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title = "A single gene target of an ETS-family transcription factor determines neuronal CO2-chemosensitivity",

abstract = "Many animals possess neurons specialized for the detection of carbon dioxide (CO(2)), which acts as a cue to elicit behavioral responses and is also an internally generated product of respiration that regulates animal physiology. In many organisms how such neurons detect CO(2) is poorly understood. We report here a mechanism that endows C. elegans neurons with the ability to detect CO(2). The ETS-5 transcription factor is necessary for the specification of CO(2)-sensing BAG neurons. Expression of a single ETS-5 target gene, gcy-9, which encodes a receptor-type guanylate cyclase, is sufficient to bypass a requirement for ets-5 in CO(2)-detection and transforms neurons into CO(2)-sensing neurons. Because ETS-5 and GCY-9 are members of gene families that are conserved between nematodes and vertebrates, a similar mechanism might act in the specification of CO(2)-sensing neurons in other phyla.",

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AU - Brandt, Julia P

AU - Aziz-Zaman, Sonya

AU - Juozaityte, Vaida

AU - Martinez-Velazquez, Luis A

AU - Petersen, Jakob Gramstrup

AU - Pocock, Roger David John

AU - Ringstad, Niels

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N2 - Many animals possess neurons specialized for the detection of carbon dioxide (CO(2)), which acts as a cue to elicit behavioral responses and is also an internally generated product of respiration that regulates animal physiology. In many organisms how such neurons detect CO(2) is poorly understood. We report here a mechanism that endows C. elegans neurons with the ability to detect CO(2). The ETS-5 transcription factor is necessary for the specification of CO(2)-sensing BAG neurons. Expression of a single ETS-5 target gene, gcy-9, which encodes a receptor-type guanylate cyclase, is sufficient to bypass a requirement for ets-5 in CO(2)-detection and transforms neurons into CO(2)-sensing neurons. Because ETS-5 and GCY-9 are members of gene families that are conserved between nematodes and vertebrates, a similar mechanism might act in the specification of CO(2)-sensing neurons in other phyla.

AB - Many animals possess neurons specialized for the detection of carbon dioxide (CO(2)), which acts as a cue to elicit behavioral responses and is also an internally generated product of respiration that regulates animal physiology. In many organisms how such neurons detect CO(2) is poorly understood. We report here a mechanism that endows C. elegans neurons with the ability to detect CO(2). The ETS-5 transcription factor is necessary for the specification of CO(2)-sensing BAG neurons. Expression of a single ETS-5 target gene, gcy-9, which encodes a receptor-type guanylate cyclase, is sufficient to bypass a requirement for ets-5 in CO(2)-detection and transforms neurons into CO(2)-sensing neurons. Because ETS-5 and GCY-9 are members of gene families that are conserved between nematodes and vertebrates, a similar mechanism might act in the specification of CO(2)-sensing neurons in other phyla.

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KW - Binding Sites

KW - Caenorhabditis elegans

KW - Caenorhabditis elegans Proteins

KW - Carbon Dioxide

KW - Gene Deletion

KW - Gene Expression Regulation

KW - Guanylate Cyclase

KW - Microscopy, Fluorescence

KW - Mutation

KW - Neurons

KW - Plasmids

KW - Proto-Oncogene Proteins c-ets

KW - Receptors, Guanylate Cyclase-Coupled

KW - Sensory Receptor Cells

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DO - 10.1371/journal.pone.0034014

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JO - PLoS Computational Biology

JF - PLoS Computational Biology

IS - 3

ER -

A single gene target of an ETS-family transcription factor determines neuronal CO2-chemosensitivity

Abstract

Keywords

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