Abstract
The enormous complexity of the central nervous system has impeded its systemic exploration for decades but powerful “omic” technologies are now pushing forward the frontiers of neuroscience research at an increasing pace. This Primer reviews the most recent progress in mass spectrometry (MS)-based proteomics, focusing on the analysis of whole proteomes, protein-based interactions, and post-translational modifications. We also discuss how advanced workflows help to unravel spatial, regulatory, and temporal aspects of neuronal systems. These tools and approaches have already led to detailed and quantitative proteomic maps of the brain and its signaling architecture, generating new insights into health and disease. We predict that these new approaches will also accelerate biomarker discovery and contribute to novel therapeutics for neurodegenerative and other brain-related diseases. Hosp and Mann describe the value of proteomics for neurobiology by reviewing recent advances in whole proteome analyses, protein-based interactions, and post-translational modifications, offering thoughtful perspectives on how to unravel spatial, regulatory, and temporal aspects of neuronal systems by proteomics.
| Original language | English |
|---|---|
| Journal | Neuron |
| Volume | 96 |
| Issue number | 3 |
| Pages (from-to) | 558-571 |
| Number of pages | 14 |
| ISSN | 0896-6273 |
| DOIs | |
| Publication status | Published - 1 Nov 2017 |
Keywords
- Animals
- Brain
- Chromatography, Liquid
- Humans
- Mass Spectrometry
- Neurons
- Neurosciences
- Proteomics
- Journal Article
- Review