A preliminary analysis of the DNA and diet of the extinct Beothuk: a systematic approach to ancient human DNA.

Melanie Kuch; Darren R Gröcke; Martin C Knyf; M Thomas P Gilbert; Ban Younghusband; Terry Young; Ingeborg Marshall; Eske Willerslev; Mark Stoneking; Hendrik Poinar

doi:10.1002/ajpa.20536

A preliminary analysis of the DNA and diet of the extinct Beothuk: a systematic approach to ancient human DNA.

Melanie Kuch, Darren R Gröcke, Martin C Knyf, M Thomas P Gilbert, Ban Younghusband, Terry Young, Ingeborg Marshall, Eske Willerslev, Mark Stoneking, Hendrik Poinar

Microbiology

27 Citations (Scopus)

Abstract

We have used a systematic protocol for extracting, quantitating, sexing and validating ancient human mitochondrial and nuclear DNA of one male and one female Beothuk, a Native American population from Newfoundland, which became extinct approximately 180 years ago. They carried mtDNA haplotypes, which fall within haplogroups X and C, consistent with Northeastern Native populations today. In addition we have sexed the male using a novel-sexing assay and confirmed the authenticity of his Y chromosome with the presence of the Native American specific Y-QM3 single nucleotide polymorphism (SNP). This is the first ancient nuclear SNP typed from a Native population in the Americas. In addition, using the same teeth we conducted a stable isotopes analysis of collagen and dentine to show that both individuals relied on marine sources (fresh and salt water fish, seals) with no hierarchy seen between them, and that their water sources were pooled or stored water. Both mtDNA sequence data and Y SNP data hint at possible gene flow or a common ancestral population for both the Beothuk and the current day Mikmaq, but more importantly the data do not lend credence to the proposed idea that the Beothuk (specifically, Nonosabasut) were of admixed (European-Native American) descent. We also analyzed patterns of DNA damage in the clones of authentic mtDNA sequences; there is no tendency for DNA damage to occur preferentially at previously defined mutational hotspots, suggesting that such mutational hotspots are not hypervariable because they are more prone to damage.
Udgivelsesdato: 2007-Apr

Original language	English
Journal	American Journal of Physical Anthropology
Volume	132
Issue number	4
Pages (from-to)	594-604
Number of pages	10
ISSN	0002-9483
DOIs	https://doi.org/10.1002/ajpa.20536
Publication status	Published - 2007

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10.1002/ajpa.20536

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@article{04620c00149511ddbee902004c4f4f50,

title = "A preliminary analysis of the DNA and diet of the extinct Beothuk: a systematic approach to ancient human DNA.",

abstract = "We have used a systematic protocol for extracting, quantitating, sexing and validating ancient human mitochondrial and nuclear DNA of one male and one female Beothuk, a Native American population from Newfoundland, which became extinct approximately 180 years ago. They carried mtDNA haplotypes, which fall within haplogroups X and C, consistent with Northeastern Native populations today. In addition we have sexed the male using a novel-sexing assay and confirmed the authenticity of his Y chromosome with the presence of the Native American specific Y-QM3 single nucleotide polymorphism (SNP). This is the first ancient nuclear SNP typed from a Native population in the Americas. In addition, using the same teeth we conducted a stable isotopes analysis of collagen and dentine to show that both individuals relied on marine sources (fresh and salt water fish, seals) with no hierarchy seen between them, and that their water sources were pooled or stored water. Both mtDNA sequence data and Y SNP data hint at possible gene flow or a common ancestral population for both the Beothuk and the current day Mikmaq, but more importantly the data do not lend credence to the proposed idea that the Beothuk (specifically, Nonosabasut) were of admixed (European-Native American) descent. We also analyzed patterns of DNA damage in the clones of authentic mtDNA sequences; there is no tendency for DNA damage to occur preferentially at previously defined mutational hotspots, suggesting that such mutational hotspots are not hypervariable because they are more prone to damage. Udgivelsesdato: 2007-Apr",

author = "Melanie Kuch and Gr{\"o}cke, {Darren R} and Knyf, {Martin C} and Gilbert, {M Thomas P} and Ban Younghusband and Terry Young and Ingeborg Marshall and Eske Willerslev and Mark Stoneking and Hendrik Poinar",

note = "Keywords: Base Sequence; Chromosomes, Human, Y; Collagen; DNA Damage; DNA Primers; DNA, Mitochondrial; Dentin; Diet; Female; Genetics, Population; Haplotypes; Humans; Indians, North American; Isotope Labeling; Male; Molecular Sequence Data; Newfoundland and Labrador; Polymorphism, Single Nucleotide; Sequence Analysis, DNA; Sex Determination (Analysis)",

year = "2007",

doi = "10.1002/ajpa.20536",

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journal = "American Journal of Physical Anthropology",

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T1 - A preliminary analysis of the DNA and diet of the extinct Beothuk: a systematic approach to ancient human DNA.

AU - Kuch, Melanie

AU - Gröcke, Darren R

AU - Knyf, Martin C

AU - Gilbert, M Thomas P

AU - Younghusband, Ban

AU - Young, Terry

AU - Marshall, Ingeborg

AU - Willerslev, Eske

AU - Stoneking, Mark

AU - Poinar, Hendrik

N1 - Keywords: Base Sequence; Chromosomes, Human, Y; Collagen; DNA Damage; DNA Primers; DNA, Mitochondrial; Dentin; Diet; Female; Genetics, Population; Haplotypes; Humans; Indians, North American; Isotope Labeling; Male; Molecular Sequence Data; Newfoundland and Labrador; Polymorphism, Single Nucleotide; Sequence Analysis, DNA; Sex Determination (Analysis)

PY - 2007

Y1 - 2007

N2 - We have used a systematic protocol for extracting, quantitating, sexing and validating ancient human mitochondrial and nuclear DNA of one male and one female Beothuk, a Native American population from Newfoundland, which became extinct approximately 180 years ago. They carried mtDNA haplotypes, which fall within haplogroups X and C, consistent with Northeastern Native populations today. In addition we have sexed the male using a novel-sexing assay and confirmed the authenticity of his Y chromosome with the presence of the Native American specific Y-QM3 single nucleotide polymorphism (SNP). This is the first ancient nuclear SNP typed from a Native population in the Americas. In addition, using the same teeth we conducted a stable isotopes analysis of collagen and dentine to show that both individuals relied on marine sources (fresh and salt water fish, seals) with no hierarchy seen between them, and that their water sources were pooled or stored water. Both mtDNA sequence data and Y SNP data hint at possible gene flow or a common ancestral population for both the Beothuk and the current day Mikmaq, but more importantly the data do not lend credence to the proposed idea that the Beothuk (specifically, Nonosabasut) were of admixed (European-Native American) descent. We also analyzed patterns of DNA damage in the clones of authentic mtDNA sequences; there is no tendency for DNA damage to occur preferentially at previously defined mutational hotspots, suggesting that such mutational hotspots are not hypervariable because they are more prone to damage. Udgivelsesdato: 2007-Apr

AB - We have used a systematic protocol for extracting, quantitating, sexing and validating ancient human mitochondrial and nuclear DNA of one male and one female Beothuk, a Native American population from Newfoundland, which became extinct approximately 180 years ago. They carried mtDNA haplotypes, which fall within haplogroups X and C, consistent with Northeastern Native populations today. In addition we have sexed the male using a novel-sexing assay and confirmed the authenticity of his Y chromosome with the presence of the Native American specific Y-QM3 single nucleotide polymorphism (SNP). This is the first ancient nuclear SNP typed from a Native population in the Americas. In addition, using the same teeth we conducted a stable isotopes analysis of collagen and dentine to show that both individuals relied on marine sources (fresh and salt water fish, seals) with no hierarchy seen between them, and that their water sources were pooled or stored water. Both mtDNA sequence data and Y SNP data hint at possible gene flow or a common ancestral population for both the Beothuk and the current day Mikmaq, but more importantly the data do not lend credence to the proposed idea that the Beothuk (specifically, Nonosabasut) were of admixed (European-Native American) descent. We also analyzed patterns of DNA damage in the clones of authentic mtDNA sequences; there is no tendency for DNA damage to occur preferentially at previously defined mutational hotspots, suggesting that such mutational hotspots are not hypervariable because they are more prone to damage. Udgivelsesdato: 2007-Apr

U2 - 10.1002/ajpa.20536

DO - 10.1002/ajpa.20536

M3 - Journal article

C2 - 17205549

SN - 0002-9483

VL - 132

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EP - 604

JO - American Journal of Physical Anthropology

JF - American Journal of Physical Anthropology

IS - 4

ER -