A peptide motif from the second fibronectin module of the neural cell adhesion molecule, NCAM, NLIKQDDGGSPIRHY, is a binding site for the FGF receptor

TY - JOUR

T1 - A peptide motif from the second fibronectin module of the neural cell adhesion molecule, NCAM, NLIKQDDGGSPIRHY, is a binding site for the FGF receptor

AU - Jacobsen, Jacob Hedemand

AU - Kiselyov, Vladislav

AU - Bock, Elisabeth

AU - Berezin, Vladimir

N1 - Keywords: Amino Acid Motifs; Amino Acid Sequence; Binding Sites; Cell Line; Humans; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; Neural Cell Adhesion Molecules; Oligopeptides; Phosphorylation; Receptors, Fibroblast Growth Factor; Surface Plasmon Resonance

PY - 2008

Y1 - 2008

N2 - The mechanism of fibroblast growth factor receptor (FGFR) activation by the neural cell adhesion molecule (NCAM) is not well understood. A motif in the second NCAM fibronectin type III (FN3) module, termed FGL, has by means of nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analyses been demonstrated to be involved in NCAM-FGFR interactions. An FGFR activation motif (FRM) in the first NCAM FN3 module also has been suggested to take part in NCAM interactions with FGFR. Here, we show for the first time that a peptide motif in the second NCAM FN3 module, different from the previously described FGL motif (NLIKQDDGGSPIRHY; termed BCL) binds and activates FGFR and induces FGFR-dependent neurite outgrowth in cultures of cerebellar granule neurons. Our results provide evidence that the BCL motif is one of the multiple FGFR binding sites in NCAM.

AB - The mechanism of fibroblast growth factor receptor (FGFR) activation by the neural cell adhesion molecule (NCAM) is not well understood. A motif in the second NCAM fibronectin type III (FN3) module, termed FGL, has by means of nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analyses been demonstrated to be involved in NCAM-FGFR interactions. An FGFR activation motif (FRM) in the first NCAM FN3 module also has been suggested to take part in NCAM interactions with FGFR. Here, we show for the first time that a peptide motif in the second NCAM FN3 module, different from the previously described FGL motif (NLIKQDDGGSPIRHY; termed BCL) binds and activates FGFR and induces FGFR-dependent neurite outgrowth in cultures of cerebellar granule neurons. Our results provide evidence that the BCL motif is one of the multiple FGFR binding sites in NCAM.

U2 - 10.1007/s11064-008-9680-2

DO - 10.1007/s11064-008-9680-2

M3 - Journal article

C2 - 18368482

SN - 0364-3190

VL - 33

SP - 2532

EP - 2539

JO - Neurochemical Research

JF - Neurochemical Research

IS - 12

ER -