A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum

TY - JOUR

T1 - A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum

AU - Walther-Larsen, Haidee

AU - Brandt, Jakob

AU - Collinge, David B.

AU - Thordal-Christensen, Hans

PY - 1993/3/1

Y1 - 1993/3/1

N2 - The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identified as a member of the 90 kDa heat shock protein (HSP90) family. The encoded protein most resembles the endoplasmic reticulum (ER) resident HSP90 protein, the 94 kDa glucose-regulated protein (GRP94) of vertebrates, as it possesses both the characteristic N-terminal domain including a signal peptide sequence and the C-terminal ER retention signal (Lys-Asp-Glu-Leu). A transcript cross-hybridizing at high stringency accumulated rapidly in leaves upon heat shock treatment. Genomic DNA blot analysis indicated the presence of a family of related genes in the barley genome.

AB - The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identified as a member of the 90 kDa heat shock protein (HSP90) family. The encoded protein most resembles the endoplasmic reticulum (ER) resident HSP90 protein, the 94 kDa glucose-regulated protein (GRP94) of vertebrates, as it possesses both the characteristic N-terminal domain including a signal peptide sequence and the C-terminal ER retention signal (Lys-Asp-Glu-Leu). A transcript cross-hybridizing at high stringency accumulated rapidly in leaves upon heat shock treatment. Genomic DNA blot analysis indicated the presence of a family of related genes in the barley genome.

KW - cDNA clone

KW - defence response

KW - Erysiphe graminis

KW - GRP94

KW - heat shock

KW - Hordeum vulgare

UR - http://www.scopus.com/inward/record.url?scp=0027571389&partnerID=8YFLogxK

U2 - 10.1007/BF00023606

DO - 10.1007/BF00023606

M3 - Journal article

C2 - 8490130

AN - SCOPUS:0027571389

SN - 0167-4412

VL - 21

SP - 1097

EP - 1108

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 6

ER -