A novel porcine model for future studies of cell-enriched fat grafting

Bo S. Rasmussen; Celine L. Sørensen; Peter V. Vester-Glowinski; Mikkel Herly; Sorel Kurbegovic; Mathias Ørholt; Jesper D. Svalgaard; Stig Frederik T. Kølle; Annemarie T. Kristensen; Maj Lis M. Talman; Krzysztof T. Drzewiecki; Anne Fischer-Nielsen

doi:10.1097/GOX.0000000000001735

A novel porcine model for future studies of cell-enriched fat grafting

Bo S. Rasmussen, Celine L. Sørensen, Peter V. Vester-Glowinski, Mikkel Herly, Sorel Kurbegovic, Mathias Ørholt, Jesper D. Svalgaard, Stig Frederik T. Kølle, Annemarie T. Kristensen, Maj Lis M. Talman, Krzysztof T. Drzewiecki, Anne Fischer-Nielsen

Department of Clinical Medicine

4 Citations (Scopus)

33 Downloads (Pure)

Abstract

Background: Cell-enriched fat grafting has shown promising results for improving graft survival, although many questions remain unanswered. A large animal model is crucial for bridging the gap between rodent studies and human trials. We present a step-by-step approach in using the Göttingen minipig as a model for future studies of cell-enriched large volume fat grafting. Methods: Fat grafting was performed as bolus injections and structural fat grafting. Graft retention was assessed by magnetic resonance imaging after 120 days. The stromal vascular fraction (SVF) was isolated from excised fat and liposuctioned fat from different anatomical sites and analyzed. Porcine adipose-derived stem/stromal cells (ASCs) were cultured in different growth supplements, and population doubling time, maximum cell yield, expression of surface markers, and differentiation potential were investigated. Results: Structural fat grafting in the breast and subcutaneous bolus grafting in the abdomen revealed average graft retention of 53.55% and 15.28%, respectively, which are similar to human reports. Liposuction yielded fewer SVF cells than fat excision, and abdominal fat had the most SVF cells/g fat with SVF yields similar to humans. Additionally, we demonstrated that porcine ASCs can be readily isolated and expanded in culture in allogeneic porcine platelet lysate and fetal bovine serum and that the use of 10% porcine platelet lysate or 20% fetal bovine serum resulted in population doubling time, maximum cell yield, surface marker profile, and trilineage differentiation that were comparable with humans. Conclusions: The Göttingen minipig is a feasible and cost-effective, large animal model for future translational studies of cell-enriched fat grafting.

Original language	English
Article number	e1735
Journal	Plastic and Reconstructive Surgery, Global Open
Volume	6
Issue number	4
ISSN	2169-7574
DOIs	https://doi.org/10.1097/GOX.0000000000001735
Publication status	Published - 2018

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A Novel Porcine Model for Future Studies of Cell-enriched Fat GraftingFinal published version, 667 KBLicence: CC BY-NC-ND

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Rasmussen, B. S., Sørensen, C. L., Vester-Glowinski, P. V., Herly, M., Kurbegovic, S., Ørholt, M., Svalgaard, J. D., Kølle, S. F. T., Kristensen, A. T., Talman, M. L. M., Drzewiecki, K. T., & Fischer-Nielsen, A. (2018). A novel porcine model for future studies of cell-enriched fat grafting. Plastic and Reconstructive Surgery, Global Open, 6(4), Article e1735. https://doi.org/10.1097/GOX.0000000000001735

Rasmussen, BS, Sørensen, CL, Vester-Glowinski, PV, Herly, M, Kurbegovic, S, Ørholt, M, Svalgaard, JD, Kølle, SFT, Kristensen, AT, Talman, MLM, Drzewiecki, KT & Fischer-Nielsen, A 2018, 'A novel porcine model for future studies of cell-enriched fat grafting', Plastic and Reconstructive Surgery, Global Open, vol. 6, no. 4, e1735. https://doi.org/10.1097/GOX.0000000000001735

@article{36953fcf48ae47bfa687a11c8b837101,

title = "A novel porcine model for future studies of cell-enriched fat grafting",

abstract = "Background: Cell-enriched fat grafting has shown promising results for improving graft survival, although many questions remain unanswered. A large animal model is crucial for bridging the gap between rodent studies and human trials. We present a step-by-step approach in using the G{\"o}ttingen minipig as a model for future studies of cell-enriched large volume fat grafting. Methods: Fat grafting was performed as bolus injections and structural fat grafting. Graft retention was assessed by magnetic resonance imaging after 120 days. The stromal vascular fraction (SVF) was isolated from excised fat and liposuctioned fat from different anatomical sites and analyzed. Porcine adipose-derived stem/stromal cells (ASCs) were cultured in different growth supplements, and population doubling time, maximum cell yield, expression of surface markers, and differentiation potential were investigated. Results: Structural fat grafting in the breast and subcutaneous bolus grafting in the abdomen revealed average graft retention of 53.55% and 15.28%, respectively, which are similar to human reports. Liposuction yielded fewer SVF cells than fat excision, and abdominal fat had the most SVF cells/g fat with SVF yields similar to humans. Additionally, we demonstrated that porcine ASCs can be readily isolated and expanded in culture in allogeneic porcine platelet lysate and fetal bovine serum and that the use of 10% porcine platelet lysate or 20% fetal bovine serum resulted in population doubling time, maximum cell yield, surface marker profile, and trilineage differentiation that were comparable with humans. Conclusions: The G{\"o}ttingen minipig is a feasible and cost-effective, large animal model for future translational studies of cell-enriched fat grafting.",

author = "Rasmussen, {Bo S.} and S{\o}rensen, {Celine L.} and Vester-Glowinski, {Peter V.} and Mikkel Herly and Sorel Kurbegovic and Mathias {\O}rholt and Svalgaard, {Jesper D.} and K{\o}lle, {Stig Frederik T.} and Kristensen, {Annemarie T.} and Talman, {Maj Lis M.} and Drzewiecki, {Krzysztof T.} and Anne Fischer-Nielsen",

year = "2018",

doi = "10.1097/GOX.0000000000001735",

language = "English",

volume = "6",

journal = "Plastic and Reconstructive Surgery, Global Open",

issn = "2169-7574",

publisher = "Lippincott Williams & Wilkins",

number = "4",

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TY - JOUR

T1 - A novel porcine model for future studies of cell-enriched fat grafting

AU - Rasmussen, Bo S.

AU - Sørensen, Celine L.

AU - Vester-Glowinski, Peter V.

AU - Herly, Mikkel

AU - Kurbegovic, Sorel

AU - Ørholt, Mathias

AU - Svalgaard, Jesper D.

AU - Kølle, Stig Frederik T.

AU - Kristensen, Annemarie T.

AU - Talman, Maj Lis M.

AU - Drzewiecki, Krzysztof T.

AU - Fischer-Nielsen, Anne

PY - 2018

Y1 - 2018

N2 - Background: Cell-enriched fat grafting has shown promising results for improving graft survival, although many questions remain unanswered. A large animal model is crucial for bridging the gap between rodent studies and human trials. We present a step-by-step approach in using the Göttingen minipig as a model for future studies of cell-enriched large volume fat grafting. Methods: Fat grafting was performed as bolus injections and structural fat grafting. Graft retention was assessed by magnetic resonance imaging after 120 days. The stromal vascular fraction (SVF) was isolated from excised fat and liposuctioned fat from different anatomical sites and analyzed. Porcine adipose-derived stem/stromal cells (ASCs) were cultured in different growth supplements, and population doubling time, maximum cell yield, expression of surface markers, and differentiation potential were investigated. Results: Structural fat grafting in the breast and subcutaneous bolus grafting in the abdomen revealed average graft retention of 53.55% and 15.28%, respectively, which are similar to human reports. Liposuction yielded fewer SVF cells than fat excision, and abdominal fat had the most SVF cells/g fat with SVF yields similar to humans. Additionally, we demonstrated that porcine ASCs can be readily isolated and expanded in culture in allogeneic porcine platelet lysate and fetal bovine serum and that the use of 10% porcine platelet lysate or 20% fetal bovine serum resulted in population doubling time, maximum cell yield, surface marker profile, and trilineage differentiation that were comparable with humans. Conclusions: The Göttingen minipig is a feasible and cost-effective, large animal model for future translational studies of cell-enriched fat grafting.

AB - Background: Cell-enriched fat grafting has shown promising results for improving graft survival, although many questions remain unanswered. A large animal model is crucial for bridging the gap between rodent studies and human trials. We present a step-by-step approach in using the Göttingen minipig as a model for future studies of cell-enriched large volume fat grafting. Methods: Fat grafting was performed as bolus injections and structural fat grafting. Graft retention was assessed by magnetic resonance imaging after 120 days. The stromal vascular fraction (SVF) was isolated from excised fat and liposuctioned fat from different anatomical sites and analyzed. Porcine adipose-derived stem/stromal cells (ASCs) were cultured in different growth supplements, and population doubling time, maximum cell yield, expression of surface markers, and differentiation potential were investigated. Results: Structural fat grafting in the breast and subcutaneous bolus grafting in the abdomen revealed average graft retention of 53.55% and 15.28%, respectively, which are similar to human reports. Liposuction yielded fewer SVF cells than fat excision, and abdominal fat had the most SVF cells/g fat with SVF yields similar to humans. Additionally, we demonstrated that porcine ASCs can be readily isolated and expanded in culture in allogeneic porcine platelet lysate and fetal bovine serum and that the use of 10% porcine platelet lysate or 20% fetal bovine serum resulted in population doubling time, maximum cell yield, surface marker profile, and trilineage differentiation that were comparable with humans. Conclusions: The Göttingen minipig is a feasible and cost-effective, large animal model for future translational studies of cell-enriched fat grafting.

U2 - 10.1097/GOX.0000000000001735

DO - 10.1097/GOX.0000000000001735

M3 - Journal article

C2 - 29876178

AN - SCOPUS:85053867572

SN - 2169-7574

VL - 6

JO - Plastic and Reconstructive Surgery, Global Open

JF - Plastic and Reconstructive Surgery, Global Open

IS - 4

M1 - e1735

ER -

A novel porcine model for future studies of cell-enriched fat grafting

Abstract

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