A novel baiting microcosm approach used to identify the bacterial community associated with Penicillium bilaii hyphae in soil

Behnoushsadat Ghodsalavi; Nanna Bygvraa Svenningsen; Xiuli Hao; Stefan Olsson; Mette Haubjerg Nicolaisen; Waleed Abu Al-Soud; Søren Johannes Sørensen; Ole Nybroe

doi:10.1371/journal.pone.0187116

A novel baiting microcosm approach used to identify the bacterial community associated with Penicillium bilaii hyphae in soil

Behnoushsadat Ghodsalavi, Nanna Bygvraa Svenningsen, Xiuli Hao, Stefan Olsson, Mette Haubjerg Nicolaisen, Waleed Abu Al-Soud, Søren Johannes Sørensen, Ole Nybroe^*

^*Corresponding author for this work

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Abstract

It is important to identify and recover bacteria associating with fungi under natural soil conditions to enable eco-physiological studies, and to facilitate the use of bacterial-fungal consortia in environmental biotechnology. We have developed a novel type of baiting microcosm, where fungal hyphae interact with bacteria under close-to-natural soil conditions; an advantage compared to model systems that determine fungal influences on bacterial communities in laboratory media. In the current approach, the hyphae are placed on a solid support, which enables the recovery of hyphae with associated bacteria in contrast to model systems that compare bulk soil and mycosphere soil. We used the baiting microcosm approach to determine, for the first time, the composition of the bacterial community associating in the soil with hyphae of the phosphate-solubilizer, Penicillium bilaii. By applying a cultivation-independent 16S rRNA gene-targeted amplicon sequencing approach, we found a hypha-associated bacterial community with low diversity compared to the bulk soil community and exhibiting massive dominance of Burkholderia OTUs. Burkholderia is known be abundant in soil environments affected by fungi, but the discovery of this massive dominance among bacteria firmly associating with hyphae in soil is novel and made possible by the current bait approach.

Original language	English
Article number	e0187116
Journal	PLOS ONE
Volume	12
Issue number	10
Number of pages	14
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0187116
Publication status	Published - 27 Oct 2017

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Ghodsalavi_2017_A_novel_baitingFinal published version, 6.06 MBLicence: CC BY

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title = "A novel baiting microcosm approach used to identify the bacterial community associated with Penicillium bilaii hyphae in soil",

abstract = "It is important to identify and recover bacteria associating with fungi under natural soil conditions to enable eco-physiological studies, and to facilitate the use of bacterial-fungal consortia in environmental biotechnology. We have developed a novel type of baiting microcosm, where fungal hyphae interact with bacteria under close-to-natural soil conditions; an advantage compared to model systems that determine fungal influences on bacterial communities in laboratory media. In the current approach, the hyphae are placed on a solid support, which enables the recovery of hyphae with associated bacteria in contrast to model systems that compare bulk soil and mycosphere soil. We used the baiting microcosm approach to determine, for the first time, the composition of the bacterial community associating in the soil with hyphae of the phosphate-solubilizer, Penicillium bilaii. By applying a cultivation-independent 16S rRNA gene-targeted amplicon sequencing approach, we found a hypha-associated bacterial community with low diversity compared to the bulk soil community and exhibiting massive dominance of Burkholderia OTUs. Burkholderia is known be abundant in soil environments affected by fungi, but the discovery of this massive dominance among bacteria firmly associating with hyphae in soil is novel and made possible by the current bait approach.",

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AU - Olsson, Stefan

AU - Nicolaisen, Mette Haubjerg

AU - Al-Soud, Waleed Abu

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A novel baiting microcosm approach used to identify the bacterial community associated with Penicillium bilaii hyphae in soil

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