A novel approach to identification of somatic retroelements' insertions in human genome

A A Kurnosov; S V Ustiugova; M V Pogorelyĭ; A Iu Komkov; D A Bolotin; K V Khodosevich; I Z Mamedov; Iu B Lebedev

A novel approach to identification of somatic retroelements' insertions in human genome

Translated title of the contribution: A novel approach to identification of somatic retroelements' insertions in human genome

A A Kurnosov, S V Ustiugova, M V Pogorelyĭ, A Iu Komkov, D A Bolotin, K V Khodosevich, I Z Mamedov, Iu B Lebedev

Abstract

The activity of retroelements is one of the factors leading to genetic variability of the modern humans. Insertions of retroelements may result in alteration of gene expression and functional diversity between cells. In recent years an increasing amount of data indicating an elevated level of retroelements' mobilisation in some human and animal tissues has been reported. Therefore, the development of a system for the detection of somatic retroposition events is required. Here we describe a novel approach to the whole-genome identification of somatic retroelemts' insertions in human genome. The developed approach was applied for the comparisons of somatic mosaicism levels in two tissues of the investigated individual. A total of 3410 insertions of retroelements belonging to AluYa5 subfamily were identified.

Translated title of the contribution	A novel approach to identification of somatic retroelements' insertions in human genome
Original language	Russian
Journal	Bioorganicheskaya Khimiya
Volume	39
Issue number	4
Pages (from-to)	466-76
Number of pages	11
ISSN	0132-3423
Publication status	Published - 2013
Externally published	Yes

Cite this

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title = "A novel approach to identification of somatic retroelements' insertions in human genome",

abstract = "The activity of retroelements is one of the factors leading to genetic variability of the modern humans. Insertions of retroelements may result in alteration of gene expression and functional diversity between cells. In recent years an increasing amount of data indicating an elevated level of retroelements' mobilisation in some human and animal tissues has been reported. Therefore, the development of a system for the detection of somatic retroposition events is required. Here we describe a novel approach to the whole-genome identification of somatic retroelemts' insertions in human genome. The developed approach was applied for the comparisons of somatic mosaicism levels in two tissues of the investigated individual. A total of 3410 insertions of retroelements belonging to AluYa5 subfamily were identified.",

keywords = "Alu Elements/genetics, DNA Transposable Elements/genetics, Genome, Human, High-Throughput Nucleotide Sequencing, Humans",

author = "Kurnosov, {A A} and Ustiugova, {S V} and Pogorelyĭ, {M V} and Komkov, {A Iu} and Bolotin, {D A} and Khodosevich, {K V} and Mamedov, {I Z} and Lebedev, {Iu B}",

year = "2013",

language = "Russisk",

volume = "39",

pages = "466--76",

journal = "Bioorganicheskaya Khimiya",

issn = "0132-3423",

publisher = "Izdatel'stvo Nauka",

number = "4",

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TY - JOUR

T1 - A novel approach to identification of somatic retroelements' insertions in human genome

AU - Kurnosov, A A

AU - Ustiugova, S V

AU - Pogorelyĭ, M V

AU - Komkov, A Iu

AU - Bolotin, D A

AU - Khodosevich, K V

AU - Mamedov, I Z

AU - Lebedev, Iu B

PY - 2013

Y1 - 2013

N2 - The activity of retroelements is one of the factors leading to genetic variability of the modern humans. Insertions of retroelements may result in alteration of gene expression and functional diversity between cells. In recent years an increasing amount of data indicating an elevated level of retroelements' mobilisation in some human and animal tissues has been reported. Therefore, the development of a system for the detection of somatic retroposition events is required. Here we describe a novel approach to the whole-genome identification of somatic retroelemts' insertions in human genome. The developed approach was applied for the comparisons of somatic mosaicism levels in two tissues of the investigated individual. A total of 3410 insertions of retroelements belonging to AluYa5 subfamily were identified.

AB - The activity of retroelements is one of the factors leading to genetic variability of the modern humans. Insertions of retroelements may result in alteration of gene expression and functional diversity between cells. In recent years an increasing amount of data indicating an elevated level of retroelements' mobilisation in some human and animal tissues has been reported. Therefore, the development of a system for the detection of somatic retroposition events is required. Here we describe a novel approach to the whole-genome identification of somatic retroelemts' insertions in human genome. The developed approach was applied for the comparisons of somatic mosaicism levels in two tissues of the investigated individual. A total of 3410 insertions of retroelements belonging to AluYa5 subfamily were identified.

KW - Alu Elements/genetics

KW - DNA Transposable Elements/genetics

KW - Genome, Human

KW - High-Throughput Nucleotide Sequencing

KW - Humans

M3 - Tidsskriftartikel

C2 - 24707728

SN - 0132-3423

VL - 39

SP - 466

EP - 476

JO - Bioorganicheskaya Khimiya

JF - Bioorganicheskaya Khimiya

IS - 4

ER -

A novel approach to identification of somatic retroelements' insertions in human genome

Abstract

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