TY - JOUR
T1 - A nitrite microsensor for profiling environmental biofilms
AU - De Beer, Dirk
AU - Schramm, Andreas
AU - Santegoeds, Cecilia M.
AU - Kühl, Michael
PY - 1997/3/1
Y1 - 1997/3/1
N2 - A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyano-cobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 μm. The response is log-linear in freshwater down to 1 μM NO2- and in seawater to 10 μM NO2-. A method is described for preparation of relatively large polyvinyl chloride (PVC)-gelled liquid membrane microsensors with a tip diameter of 5 to 15 μm, having a hydrophilic coating on the tip. The coating and increased tip diameter resulted in more sturdy sensors, with a lower detection limit and a more stable signal than uncoated nitrite sensors with a tip diameter of 1 to 3 μm. The coating protects the sensor membrane from detrimental direct contact with biomass and can be used for all PVC-gelled liquid membrane sensors meant for profiling microbial mats, biofilms, and sediments. Thanks to these improvements, liquid membrane sensors can now be used in complex environmental samples and in situ, e.g., in operating bioreactors. Examples of measurements in denitrifying, nitrifying, and nitrifying/denitrifying biofilms from wastewater treatment plants are shown. In all of these biofilms high nitrite concentrations were found in narrow zones of less than 1 mm.
AB - A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyano-cobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 μm. The response is log-linear in freshwater down to 1 μM NO2- and in seawater to 10 μM NO2-. A method is described for preparation of relatively large polyvinyl chloride (PVC)-gelled liquid membrane microsensors with a tip diameter of 5 to 15 μm, having a hydrophilic coating on the tip. The coating and increased tip diameter resulted in more sturdy sensors, with a lower detection limit and a more stable signal than uncoated nitrite sensors with a tip diameter of 1 to 3 μm. The coating protects the sensor membrane from detrimental direct contact with biomass and can be used for all PVC-gelled liquid membrane sensors meant for profiling microbial mats, biofilms, and sediments. Thanks to these improvements, liquid membrane sensors can now be used in complex environmental samples and in situ, e.g., in operating bioreactors. Examples of measurements in denitrifying, nitrifying, and nitrifying/denitrifying biofilms from wastewater treatment plants are shown. In all of these biofilms high nitrite concentrations were found in narrow zones of less than 1 mm.
UR - http://www.scopus.com/inward/record.url?scp=0031030476&partnerID=8YFLogxK
M3 - Journal article
AN - SCOPUS:0031030476
SN - 0099-2240
VL - 63
SP - 973
EP - 977
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 3
ER -
