A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins

Pil Seok Chae; Søren G F Rasmussen; Rohini R Rana; Kamil Gotfryd; Andrew C Kruse; Aashish Manglik; Kyung Ho Cho; Shailika Nurva; Ulrik Gether; Lan Guan; Claus J Loland; Bernadette Byrne; Brian K Kobilka; Samuel H Gellman

doi:10.1002/chem.201200069

A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins

Pil Seok Chae, Søren G F Rasmussen, Rohini R Rana, Kamil Gotfryd, Andrew C Kruse, Aashish Manglik, Kyung Ho Cho, Shailika Nurva, Ulrik Gether, Lan Guan, Claus J Loland, Bernadette Byrne, Brian K Kobilka, Samuel H Gellman

77 Citations (Scopus)

Abstract

Non-traditional amphiphiles: Conferring aqueous solubility on membrane proteins generally requires the use of a detergent or other amphiphilic agent. A new class of amphiphiles was synthesized, based on steroidal lipophilic groups, and evaluated with several membrane proteins. The results show that the new amphiphile, "glyco-diosgenin" (GDN; see figure), confers enhanced stability to a variety of membrane proteins in solution relative to popular conventional detergents, such as dodecylmaltoside (DDM).

Original language	English
Journal	Chemistry: A European Journal
Volume	18
Issue number	31
Pages (from-to)	9485-90
Number of pages	6
ISSN	0947-6539
DOIs	https://doi.org/10.1002/chem.201200069
Publication status	Published - 27 Jul 2012

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Chae, P. S., Rasmussen, S. G. F., Rana, R. R., Gotfryd, K., Kruse, A. C., Manglik, A., Cho, K. H., Nurva, S., Gether, U., Guan, L., Loland, C. J., Byrne, B., Kobilka, B. K., & Gellman, S. H. (2012). A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins. Chemistry: A European Journal, 18(31), 9485-90. https://doi.org/10.1002/chem.201200069

Chae, PS, Rasmussen, SGF, Rana, RR, Gotfryd, K, Kruse, AC, Manglik, A, Cho, KH, Nurva, S, Gether, U, Guan, L, Loland, CJ, Byrne, B, Kobilka, BK & Gellman, SH 2012, 'A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins', Chemistry: A European Journal, vol. 18, no. 31, pp. 9485-90. https://doi.org/10.1002/chem.201200069

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abstract = "Non-traditional amphiphiles: Conferring aqueous solubility on membrane proteins generally requires the use of a detergent or other amphiphilic agent. A new class of amphiphiles was synthesized, based on steroidal lipophilic groups, and evaluated with several membrane proteins. The results show that the new amphiphile, {"}glyco-diosgenin{"} (GDN; see figure), confers enhanced stability to a variety of membrane proteins in solution relative to popular conventional detergents, such as dodecylmaltoside (DDM).",

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AU - Rana, Rohini R

AU - Gotfryd, Kamil

AU - Kruse, Andrew C

AU - Manglik, Aashish

AU - Cho, Kyung Ho

AU - Nurva, Shailika

AU - Gether, Ulrik

AU - Guan, Lan

AU - Loland, Claus J

AU - Byrne, Bernadette

AU - Kobilka, Brian K

AU - Gellman, Samuel H

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JO - Chemistry: A European Journal

JF - Chemistry: A European Journal

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ER -

A New Class of Amphiphiles Bearing Rigid Hydrophobic Groups for Solubilization and Stabilization of Membrane Proteins

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