@inbook{f291726adb39455b92f282bfb6bcc8bc,
title = "A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation",
abstract = "Site-specific arrest of DNA replication is a useful tool for analyzing cellular responses to DNA replication perturbation. The E. coli Tus-Ter replication barrier can be reconstituted in eukaryotic cells as a system to engineer an unscheduled collision between a replication fork and an {"}alien{"} impediment to DNA replication. To further develop this system as a versatile tool, we describe a set of reagents and a detailed protocol that can be used to engineer Tus-Ter barriers into any locus in the budding yeast genome. Because the Tus-Ter complex is a bipartite system with intrinsic DNA replication-blocking activity, the reagents and protocols developed and validated in yeast could also be optimized to engineer site-specific replication fork barriers into other eukaryotic cell types.",
author = "Larsen, {Nicolai B} and Hickson, {Ian D} and Mankouri, {Hocine W}",
year = "2018",
doi = "10.1007/978-1-4939-7306-4_20",
language = "English",
isbn = "978-1-4939-7305-7",
volume = "1672",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer",
pages = "295--309",
booktitle = "Genome Instability",
}