A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples

Gerhard Holst; Oliver Kohls; Ingo Klimant; Bettina König; Michael Kühl; Thomas Richter

doi:10.1016/S0925-4005(98)00232-9

A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples

Gerhard Holst^*, Oliver Kohls, Ingo Klimant, Bettina König, Michael Kühl, Thomas Richter

^*Corresponding author for this work

Marine Biology

121 Citations (Scopus)

Abstract

We developed a new modular luminescence lifetime imaging system (MOLLI), that enables the imaging of luminescence lifetimes in the range of 1 μs to 1 s. The system can easily be adapted to different experimental applications. The central parts of the system are a recently released CCD-camera with a fast electronic shutter and gated LED (light emitting diode) or Xe excitation light sources. A personal computer controls the gating and image acquisition via a pulse delay generator. Here we present the new imaging system and give examples of its performance when used for measuring two-dimensional oxygen distributions with planar optodes. Furthermore, future applications of the system in biology are discussed.

Original language	English
Journal	Sensors and Actuators, B: Chemical
Volume	51
Issue number	1-3
Pages (from-to)	163-170
Number of pages	8
ISSN	0925-4005
DOIs	https://doi.org/10.1016/S0925-4005(98)00232-9
Publication status	Published - 31 Aug 1998

Keywords

Fluorescence lifetime imaging
Luminescence lifetime imaging
Oxygen optode
Planar optode

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10.1016/S0925-4005(98)00232-9

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abstract = "We developed a new modular luminescence lifetime imaging system (MOLLI), that enables the imaging of luminescence lifetimes in the range of 1 μs to 1 s. The system can easily be adapted to different experimental applications. The central parts of the system are a recently released CCD-camera with a fast electronic shutter and gated LED (light emitting diode) or Xe excitation light sources. A personal computer controls the gating and image acquisition via a pulse delay generator. Here we present the new imaging system and give examples of its performance when used for measuring two-dimensional oxygen distributions with planar optodes. Furthermore, future applications of the system in biology are discussed.",

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AU - Holst, Gerhard

AU - Kohls, Oliver

AU - Klimant, Ingo

AU - König, Bettina

AU - Kühl, Michael

AU - Richter, Thomas

PY - 1998/8/31

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AB - We developed a new modular luminescence lifetime imaging system (MOLLI), that enables the imaging of luminescence lifetimes in the range of 1 μs to 1 s. The system can easily be adapted to different experimental applications. The central parts of the system are a recently released CCD-camera with a fast electronic shutter and gated LED (light emitting diode) or Xe excitation light sources. A personal computer controls the gating and image acquisition via a pulse delay generator. Here we present the new imaging system and give examples of its performance when used for measuring two-dimensional oxygen distributions with planar optodes. Furthermore, future applications of the system in biology are discussed.

KW - Fluorescence lifetime imaging

KW - Luminescence lifetime imaging

KW - Oxygen optode

KW - Planar optode

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JO - Sensors and Actuators B: Chemical

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A modular luminescence lifetime imaging system for mapping oxygen distribution in biological samples

Abstract

Keywords

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